Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   NPA28_RS13025 Genome accession   NZ_CP101718
Coordinates   2574007..2574774 (-) Length   255 a.a.
NCBI ID   WP_215510330.1    Uniprot ID   -
Organism   Bacillus halotolerans strain S-5     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2569007..2579774
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NPA28_RS13005 (NPA28_13005) - 2569178..2569990 (-) 813 WP_044154519.1 pyruvate, water dikinase regulatory protein -
  NPA28_RS13010 (NPA28_13010) ccpN 2570016..2570654 (-) 639 WP_024122084.1 transcriptional regulator CcpN -
  NPA28_RS13015 (NPA28_13015) glyS 2570788..2572827 (-) 2040 WP_217827791.1 glycine--tRNA ligase subunit beta -
  NPA28_RS13020 (NPA28_13020) glyQ 2572820..2573707 (-) 888 WP_010334976.1 glycine--tRNA ligase subunit alpha -
  NPA28_RS13025 (NPA28_13025) recO 2574007..2574774 (-) 768 WP_215510330.1 DNA repair protein RecO Machinery gene
  NPA28_RS13030 (NPA28_13030) - 2574811..2574954 (-) 144 WP_024122087.1 YqzL family protein -
  NPA28_RS13035 (NPA28_13035) era 2575098..2576003 (-) 906 WP_215510329.1 GTPase Era -
  NPA28_RS13040 (NPA28_13040) cdd 2575984..2576394 (-) 411 WP_024122088.1 cytidine deaminase -
  NPA28_RS13045 (NPA28_13045) - 2576508..2576885 (-) 378 WP_059335436.1 diacylglycerol kinase family protein -
  NPA28_RS13050 (NPA28_13050) ybeY 2576866..2577339 (-) 474 WP_010334982.1 rRNA maturation RNase YbeY -
  NPA28_RS13055 (NPA28_13055) pgpH 2577340..2579475 (-) 2136 WP_081638339.1 cyclic-di-AMP phosphodiesterase PgpH -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29275.00 Da        Isoelectric Point: 8.5592

>NTDB_id=711769 NPA28_RS13025 WP_215510330.1 2574007..2574774(-) (recO) [Bacillus halotolerans strain S-5]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGSFLMQKTTGLGTLQQGEMILSMRG
IREDLFLTAYAAFIAELVDKGTEEKKPNPYLFEFILESFKQLDEGTDPDVITFIVQMKMLAVMGLYPELNHCVHCKSQGG
TFHFSVRDNGFICHRCFDKDPYKVPIKPQTARLLRLFYYFDLSRIGNVSLKQETKNELKQVIDLYYEEYSGVYLKSKRFL
DQMESMKHLMGENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=711769 NPA28_RS13025 WP_215510330.1 2574007..2574774(-) (recO) [Bacillus halotolerans strain S-5]
ATGCTGACAAAATGTGAAGGAATCGTTCTTCGTACCAATGATTACGGAGAGACGAATAAAATTGTTACTTTACTGACAAG
AGAACACGGAAAAATAGGTGTTATGGCCAGAGGAGCCAAAAAACCGAACAGCCGTTTATCAGCAGTCAGCCAGCCCTTTT
TGTATGGTTCATTCCTAATGCAGAAAACAACAGGCCTCGGAACCCTTCAGCAGGGTGAAATGATCCTCAGCATGAGAGGC
ATCAGAGAGGATTTGTTTTTAACTGCATATGCAGCTTTTATTGCTGAGCTCGTTGACAAAGGCACAGAAGAAAAAAAACC
AAATCCCTATTTGTTTGAATTTATTTTAGAATCCTTTAAACAGCTTGACGAAGGAACAGACCCGGATGTTATTACCTTCA
TTGTTCAAATGAAGATGCTTGCTGTGATGGGACTGTATCCTGAGCTTAATCACTGTGTGCATTGTAAAAGCCAGGGTGGG
ACATTCCATTTTTCTGTTCGCGATAACGGTTTTATTTGCCATCGCTGCTTTGACAAAGACCCTTACAAGGTGCCGATTAA
GCCGCAAACGGCAAGGCTTTTGAGACTGTTTTATTACTTCGACCTTTCAAGGATTGGCAATGTGTCTTTAAAGCAGGAAA
CGAAAAATGAATTAAAGCAAGTTATTGACTTGTACTACGAAGAATATTCAGGGGTTTATCTGAAATCGAAGCGTTTTTTA
GATCAAATGGAGAGCATGAAACATCTTATGGGTGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-75)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

94.118

100

0.941