Detailed information NTG0711751

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: POF64_RS01160
• Genome accession: NZ_CP116713
• Coordinates: 200056..200517 (+)
• Length: 153 a.a.
• NCBI ID: WP_001874812.1
• Uniprot ID: -
• Organism: Streptococcus agalactiae strain A909 Cas9 AEKOrevertant
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 195056..205517

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
POF64_RS01130 (POF64_01130)	-	196671..197036 (+)	366	WP_000285373.1	DUF1033 family protein	-
POF64_RS01135 (POF64_01135)	comGA	197209..198180 (+)	972	WP_000250805.1	competence type IV pilus ATPase ComGA	Machinery gene
POF64_RS01140 (POF64_01140)	comGB	198074..199117 (+)	1044	WP_223295889.1	competence type IV pilus assembly protein ComGB	Machinery gene
POF64_RS01145 (POF64_01145)	comGC/cglC	199114..199443 (+)	330	WP_000793380.1	competence type IV pilus major pilin ComGC	Machinery gene
POF64_RS01150 (POF64_01150)	comGD	199418..199831 (+)	414	WP_000244261.1	competence type IV pilus minor pilin ComGD	Machinery gene
POF64_RS01155 (POF64_01155)	comGE	199803..200102 (+)	300	WP_000234804.1	competence type IV pilus minor pilin ComGE	Machinery gene
POF64_RS01160 (POF64_01160)	comGF	200056..200517 (+)	462	WP_001874812.1	competence type IV pilus minor pilin ComGF	Machinery gene
POF64_RS01165 (POF64_01165)	comGG	200495..200866 (+)	372	WP_000601104.1	competence type IV pilus minor pilin ComGG	Machinery gene
POF64_RS01170 (POF64_01170)	comGH	200981..201955 (+)	975	WP_001008574.1	N-6 DNA methylase	Machinery gene
POF64_RS01175 (POF64_01175)	-	201987..203180 (+)	1194	WP_000047534.1	acetate kinase	-
POF64_RS01180 (POF64_01180)	-	203332..203538 (+)	207	WP_000798241.1	helix-turn-helix transcriptional regulator	-
POF64_RS01185 (POF64_01185)	-	203597..203734 (+)	138	WP_001865900.1	hypothetical protein	-
POF64_RS01190 (POF64_01190)	-	203775..204230 (+)	456	WP_000905673.1	hypothetical protein	-
POF64_RS01195 (POF64_01195)	-	204299..204964 (+)	666	WP_000008113.1	type II CAAX endopeptidase family protein	-

Sequence

Protein

• Length: 153 a.a.
• Molecular weight: 17875.89 Da
• Isoelectric Point: 9.8392

>NTDB_id=711751 POF64_RS01160 WP_001874812.1 200056..200517(+) (comGF) [Streptococcus agalactiae strain A909 Cas9 AEKOrevertant]
MKIIRKFCTYQKLRVKAFTLLECLVALVTITGALLVYQGLTKLLAQQIVVMSSSSQSEWVLLTQQLNAEFEGAHLEYLRQ
NKLYLRKQDKIVTFGKSNKDDFRKTGYDGRGYQPMVYGLDNCQMSQTKSMVKLVFYFKDGLKRTFYYDFKEET

Nucleotide

• Length: 462 bp

>NTDB_id=711751 POF64_RS01160 WP_001874812.1 200056..200517(+) (comGF) [Streptococcus agalactiae strain A909 Cas9 AEKOrevertant]
TTGAAGATAATAAGGAAATTTTGCACGTATCAAAAGTTAAGGGTTAAAGCATTTACGCTTTTAGAATGTTTGGTAGCATT
GGTTACAATCACAGGAGCTTTACTAGTTTATCAAGGACTGACAAAATTGTTGGCTCAACAGATAGTAGTGATGTCTTCTT
CCAGTCAGTCTGAATGGGTGTTATTAACTCAGCAACTAAATGCAGAATTTGAAGGCGCTCATCTGGAATATTTAAGACAG
AACAAACTTTATTTACGTAAGCAAGATAAGATTGTAACCTTTGGCAAATCTAATAAAGATGATTTCCGTAAGACAGGTTA
TGATGGTCGAGGTTATCAACCAATGGTTTATGGGTTAGACAATTGTCAAATGAGTCAGACCAAAAGTATGGTAAAACTTG
TTTTTTATTTTAAGGACGGGTTAAAAAGGACATTTTACTATGATTTTAAAGAAGAAACTTAA

Domains

Predicted by InterProScan.

• Putative Competence protein ComGF (ComGF) (49-142)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Streptococcus mutans UA159	47.887	92.81	0.444
comGF	Streptococcus mutans UA140	47.887	92.81	0.444
comGF/cglF	Streptococcus pneumoniae Rx1	43.165	90.85	0.392
comGF/cglF	Streptococcus pneumoniae D39	43.165	90.85	0.392
comGF/cglF	Streptococcus pneumoniae R6	43.165	90.85	0.392
comGF/cglF	Streptococcus pneumoniae TIGR4	43.165	90.85	0.392
comGF/cglF	Streptococcus mitis SK321	44.03	87.582	0.386
comGF/cglF	Streptococcus mitis NCTC 12261	43.284	87.582	0.379
comGF	Lactococcus lactis subsp. cremoris KW2	40.714	91.503	0.373