Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   NPA28_RS01980 Genome accession   NZ_CP101718
Coordinates   369717..370154 (-) Length   145 a.a.
NCBI ID   WP_069487487.1    Uniprot ID   -
Organism   Bacillus halotolerans strain S-5     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 364717..375154
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NPA28_RS01945 (NPA28_01945) - 365276..365700 (+) 425 Protein_339 RDD family protein -
  NPA28_RS01950 (NPA28_01950) - 365812..366117 (+) 306 WP_370542280.1 YckD family protein -
  NPA28_RS01955 (NPA28_01955) - 366257..366676 (+) 420 WP_024120173.1 MarR family winged helix-turn-helix transcriptional regulator -
  NPA28_RS01960 (NPA28_01960) - 366667..367005 (+) 339 WP_059334471.1 DUF3147 family protein -
  NPA28_RS01965 (NPA28_01965) - 367022..367750 (+) 729 WP_256766629.1 AAA family ATPase -
  NPA28_RS01970 (NPA28_01970) - 367823..369256 (+) 1434 WP_044161931.1 glycoside hydrolase family 1 protein -
  NPA28_RS01975 (NPA28_01975) nin/comJ 369292..369690 (-) 399 WP_024120177.1 competence protein ComJ Regulator
  NPA28_RS01980 (NPA28_01980) nucA/comI 369717..370154 (-) 438 WP_069487487.1 DNA-entry nuclease Machinery gene
  NPA28_RS01985 (NPA28_01985) tlpC 370340..372064 (-) 1725 WP_256766630.1 methyl-accepting chemotaxis protein TlpC -
  NPA28_RS01990 (NPA28_01990) hxlB 372173..372730 (-) 558 WP_256766631.1 6-phospho-3-hexuloisomerase -
  NPA28_RS01995 (NPA28_01995) hxlA 372736..373368 (-) 633 WP_003224929.1 3-hexulose-6-phosphate synthase -
  NPA28_RS02000 (NPA28_02000) hxlR 373600..373962 (+) 363 WP_044161922.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16042.79 Da        Isoelectric Point: 4.2623

>NTDB_id=711726 NPA28_RS01980 WP_069487487.1 369717..370154(-) (nucA/comI) [Bacillus halotolerans strain S-5]
MDIIKTLLLVIVVIAAAAVGLIKGDFFSDDQKTSQTEEYDETLAFPSERYPETANHIKDAIREGHSDVCTIDRDGAEERR
EQSLKDVPSKTGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 438 bp        

>NTDB_id=711726 NPA28_RS01980 WP_069487487.1 369717..370154(-) (nucA/comI) [Bacillus halotolerans strain S-5]
ATGGACATTATAAAAACTTTACTTCTCGTAATAGTCGTCATAGCAGCTGCAGCTGTTGGCCTGATTAAGGGAGACTTTTT
CTCAGACGATCAAAAAACGTCTCAAACAGAAGAATATGACGAAACACTGGCCTTCCCTTCTGAGCGTTATCCTGAGACAG
CAAATCATATCAAGGACGCAATACGTGAGGGGCATTCAGATGTGTGCACCATAGACAGAGACGGAGCTGAGGAACGCCGC
GAACAATCATTAAAAGACGTACCTTCCAAAACAGGGTATGACCGAGATGAATGGCCAATGGCCATGTGCAAAGAAGGCGG
TGAAGGGGCTTCTGTAGAATATATTTCTCCCGCTGACAATCGCGGCGCAGGCTCCTGGGTCGGGCATCAGCTGACCGATT
ACCCTGACGGCACAAAGGTTTTATTTACGATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

92.361

99.31

0.917