Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   NO469_RS05855 Genome accession   NZ_CP101685
Coordinates   1227530..1228903 (-) Length   457 a.a.
NCBI ID   WP_250200494.1    Uniprot ID   -
Organism   Ligilactobacillus salivarius strain L.S.05     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1222530..1233903
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NO469_RS05835 (NO469_05835) - 1222808..1223215 (-) 408 WP_250200492.1 Mini-ribonuclease 3 -
  NO469_RS05840 (NO469_05840) cysS 1223208..1224620 (-) 1413 WP_250200493.1 cysteine--tRNA ligase -
  NO469_RS05845 (NO469_05845) gltX 1224774..1226261 (-) 1488 WP_003700687.1 glutamate--tRNA ligase -
  NO469_RS05850 (NO469_05850) - 1226392..1227504 (-) 1113 WP_003700688.1 PIN/TRAM domain-containing protein -
  NO469_RS05855 (NO469_05855) radA 1227530..1228903 (-) 1374 WP_250200494.1 DNA repair protein RadA Machinery gene
  NO469_RS05860 (NO469_05860) - 1228921..1229457 (-) 537 WP_250200495.1 dUTP diphosphatase -
  NO469_RS05865 (NO469_05865) - 1229613..1229891 (+) 279 WP_003702618.1 GNAT family N-acetyltransferase -
  NO469_RS05870 (NO469_05870) - 1229980..1231314 (+) 1335 WP_003706508.1 aminopeptidase C -
  NO469_RS05875 (NO469_05875) - 1231527..1231880 (+) 354 WP_223687770.1 bacteriocin immunity protein -
  NO469_RS05880 (NO469_05880) - 1232050..1232724 (-) 675 WP_003706506.1 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase -
  NO469_RS05885 (NO469_05885) - 1232746..1233690 (-) 945 WP_160944262.1 AEC family transporter -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 49732.41 Da        Isoelectric Point: 8.0254

>NTDB_id=711517 NO469_RS05855 WP_250200494.1 1227530..1228903(-) (radA) [Ligilactobacillus salivarius strain L.S.05]
MAKTKTRYVCQNCEYISPRYLGRCPNCGSWNSLVEEIEKKESPTKAQPRVSISGTTVKPKLIDDVSTVETPRFKTNLEEL
NRVLGGGIVPGSLVLIGGDPGIGKSTLLLQVSGQLSTLGKKVLYVSGEESATQIKLRADRLGVHGTELYLYPETDMDSIR
KNIELLKPDFVVIDSVQTMQEPEMTAAVGSVSQIREVTGDLMQIAKTNGITVFVVGHVTKGGAIAGPKILEHMVDTVLYF
EGDQHRTFRILRAVKNRFGSTNEIGIFEMKNGGLSEVANPSEIFLEERLKGATGSAVVASLEGTRPILVELQALVTPTAF
GNAKRTTTGLDHNRVALLMAVLEKRAGLLLQNQDAYLKAAGGVKLDEPAIDLAIAVSLVSSYRDAETRPTDCFIGEVGLT
GEIRRVNRIEQRVVEAAKLGFKRVFLPKNNLDGWMPPKEIEVVGVSTLNQALKLIFS

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=711517 NO469_RS05855 WP_250200494.1 1227530..1228903(-) (radA) [Ligilactobacillus salivarius strain L.S.05]
TTGGCGAAAACTAAGACAAGATATGTTTGTCAAAATTGTGAATATATCTCTCCACGTTATTTAGGACGTTGCCCTAATTG
TGGTTCTTGGAATAGCTTGGTAGAGGAAATAGAGAAAAAGGAAAGTCCAACTAAAGCACAACCTAGAGTTAGTATCTCTG
GTACAACAGTGAAACCTAAATTAATTGATGATGTTTCTACTGTAGAAACTCCAAGATTTAAAACTAATTTAGAAGAATTG
AATCGTGTTTTAGGTGGCGGAATTGTTCCCGGATCTTTAGTGTTAATTGGTGGAGATCCAGGTATTGGCAAGTCTACATT
ATTACTACAAGTTTCTGGACAATTATCTACTCTCGGGAAAAAGGTTCTTTATGTTTCTGGGGAAGAAAGTGCAACTCAAA
TTAAATTACGTGCGGATCGTTTAGGAGTTCATGGAACTGAGCTATATTTATATCCTGAAACAGATATGGATAGTATTAGA
AAAAATATTGAACTTTTAAAGCCTGATTTTGTTGTAATCGATTCGGTGCAGACAATGCAGGAGCCAGAAATGACAGCCGC
AGTTGGAAGTGTTTCTCAAATTAGAGAAGTAACTGGAGATTTGATGCAAATTGCGAAAACTAATGGGATAACTGTCTTTG
TTGTAGGACATGTTACTAAAGGTGGAGCAATTGCAGGACCTAAAATTTTAGAACATATGGTAGATACTGTTCTTTATTTT
GAGGGAGATCAACATAGAACATTTAGAATTTTACGAGCGGTTAAAAATAGATTTGGTTCAACTAATGAAATTGGAATCTT
TGAAATGAAAAATGGAGGATTAAGCGAAGTTGCTAATCCATCAGAGATTTTTCTAGAAGAAAGATTAAAAGGGGCTACGG
GATCAGCTGTCGTTGCTTCTCTTGAAGGAACTAGACCCATATTGGTAGAATTACAAGCACTAGTAACACCGACTGCATTT
GGAAATGCTAAGCGAACAACAACTGGTTTAGATCACAATCGAGTTGCGTTACTGATGGCAGTACTTGAAAAAAGAGCTGG
TTTGTTATTGCAAAATCAGGATGCTTATTTGAAGGCTGCTGGTGGAGTGAAGTTAGATGAGCCAGCAATAGATTTAGCCA
TTGCTGTTAGTTTAGTTTCTAGTTATCGCGATGCAGAAACAAGACCAACTGACTGTTTTATAGGGGAAGTGGGATTAACT
GGTGAAATAAGACGAGTTAATCGTATTGAACAGCGTGTTGTTGAGGCTGCTAAGCTAGGATTTAAGCGAGTATTTTTACC
AAAGAACAACTTAGATGGTTGGATGCCTCCAAAAGAGATTGAAGTAGTAGGCGTAAGTACACTAAATCAAGCATTAAAAC
TAATTTTTAGTTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(364-430)

AAA domain (AAA_25)

(67-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

68.49

100

0.685
  radA Streptococcus mitis SK321

68.271

100

0.683
  radA Streptococcus pneumoniae Rx1

68.271

100

0.683
  radA Streptococcus pneumoniae D39

68.271

100

0.683
  radA Streptococcus pneumoniae R6

68.271

100

0.683
  radA Streptococcus pneumoniae TIGR4

68.271

100

0.683
  radA Bacillus subtilis subsp. subtilis str. 168

66.3

99.344

0.659