Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   NO220_RS17655 Genome accession   NZ_CP101661
Coordinates   3569519..3570664 (+) Length   381 a.a.
NCBI ID   WP_088037228.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain B408     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 3564519..3575664
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NO220_RS17635 (NO220_17635) - 3564993..3565709 (-) 717 WP_003151075.1 Crp/Fnr family transcriptional regulator -
  NO220_RS17640 (NO220_17640) narK 3565807..3566997 (-) 1191 WP_043867514.1 nitrate transporter NarK -
  NO220_RS17645 (NO220_17645) argS 3567132..3568802 (-) 1671 WP_003151071.1 arginine--tRNA ligase -
  NO220_RS17650 (NO220_17650) - 3568799..3569227 (-) 429 WP_207579577.1 DUF1934 domain-containing protein -
  NO220_RS17655 (NO220_17655) rapF 3569519..3570664 (+) 1146 WP_088037228.1 tetratricopeptide repeat protein Regulator
  NO220_RS17660 (NO220_17660) - 3570648..3570767 (+) 120 WP_003151066.1 PhrC/PhrF family phosphatase-inhibitory pheromone -
  NO220_RS17665 (NO220_17665) - 3570966..3571169 (+) 204 WP_003151064.1 helix-turn-helix transcriptional regulator -
  NO220_RS17670 (NO220_17670) - 3571183..3571386 (+) 204 WP_003151062.1 hypothetical protein -
  NO220_RS17675 (NO220_17675) - 3571421..3571606 (+) 186 Protein_3424 DUF4177 domain-containing protein -
  NO220_RS17680 (NO220_17680) - 3571794..3572075 (+) 282 WP_014306021.1 hypothetical protein -
  NO220_RS17685 (NO220_17685) - 3572119..3572631 (+) 513 WP_003151059.1 stage II sporulation protein M -
  NO220_RS17690 (NO220_17690) - 3573222..3573440 (-) 219 WP_003151056.1 LuxR C-terminal-related transcriptional regulator -
  NO220_RS17695 (NO220_17695) speB 3573610..3574482 (-) 873 WP_003151054.1 agmatinase -
  NO220_RS17700 (NO220_17700) speE 3574542..3575372 (-) 831 WP_003151052.1 spermidine synthase -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45325.46 Da        Isoelectric Point: 4.9264

>NTDB_id=711468 NO220_RS17655 WP_088037228.1 3569519..3570664(+) (rapF) [Bacillus amyloliquefaciens strain B408]
MASVLSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEDDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLADIDKKQARLTGRLDYYFNFFRGMYELDQREYLSAIKFFKKAETKLSFIKDTIDKAEYFFKMSEVYYYLKQTYVS
TDYARQAYEIYKAQSSYDIRLIQCQSLFGANFLDLKLYDRAITHFQKAFSLAETEKQPQLMGRTLYNIALCKQSLNDYEA
AITYVEKAIDVFESAKMTPSLPQAYFLMTQIHYKLERYDKALEYHKKGVIYSEESADSVYLHEFEFLKSLYVNGPDEDAM
KRSFDFLESKMMYADIEDFALDAAKYYHKLEDYKKASTYFLKVEEARQHIQGGVTFYEAEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=711468 NO220_RS17655 WP_088037228.1 3569519..3570664(+) (rapF) [Bacillus amyloliquefaciens strain B408]
GTGGCGAGTGTTTTATCGTCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAACTTGCAGGCCTGGAAGATGACCAGGATCTTCATCTGTACTATT
CTCTCATGGAATTCCGCCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGATCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGGCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGATCAGCGGGAATATTTGTCGGCTATAAAATTTTTTAAAAAGGCTGAGACGAAACTCTCATTTA
TTAAGGATACTATTGATAAAGCCGAGTACTTTTTCAAGATGTCTGAAGTCTACTACTATTTGAAACAAACGTATGTATCT
ACCGATTACGCAAGGCAGGCATATGAAATATATAAAGCTCAGTCGTCATACGATATCAGGCTCATACAATGCCAATCCTT
ATTCGGAGCAAATTTCCTGGATTTAAAACTATATGATCGGGCAATTACTCACTTTCAAAAGGCTTTTTCTTTGGCAGAAA
CAGAAAAACAGCCTCAATTAATGGGCAGGACGCTGTATAACATCGCACTTTGCAAACAAAGCCTGAACGACTATGAAGCA
GCTATAACTTATGTTGAAAAAGCCATTGATGTTTTTGAAAGTGCTAAAATGACCCCTTCTTTGCCCCAAGCATATTTTTT
AATGACACAGATTCATTATAAATTAGAAAGATACGACAAAGCGCTCGAGTATCATAAAAAAGGAGTTATCTATTCCGAAG
AGTCAGCAGATTCAGTTTATTTACATGAATTCGAATTTTTAAAATCTTTGTATGTGAACGGACCTGATGAGGATGCAATG
AAAAGGAGTTTTGACTTTTTGGAAAGTAAGATGATGTATGCAGATATAGAAGATTTTGCTCTTGATGCAGCTAAATATTA
TCACAAACTTGAAGATTATAAAAAAGCTTCAACATACTTTTTAAAAGTTGAAGAAGCAAGACAGCATATTCAAGGGGGGG
TAACTTTTTATGAAGCTGAAGTATAA

Domains

Predicted by InterproScan.

response regulator aspartate phosphatase H, N terminal (RapH_N)

(5-65)

Tetratricopeptide repeat (TPR_12)

(223-287)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

74.541

100

0.745
  rapC Bacillus subtilis subsp. subtilis str. 168

53.158

99.738

0.53