Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   NO220_RS12175 Genome accession   NZ_CP101661
Coordinates   2507780..2508547 (-) Length   255 a.a.
NCBI ID   WP_003152982.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain B408     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2502780..2513547
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NO220_RS12155 (NO220_12155) - 2502982..2503794 (-) 813 WP_003152991.1 pyruvate, water dikinase regulatory protein -
  NO220_RS12160 (NO220_12160) ccpN 2503820..2504458 (-) 639 WP_003152989.1 transcriptional regulator CcpN -
  NO220_RS12165 (NO220_12165) glyS 2504559..2506598 (-) 2040 WP_256078188.1 glycine--tRNA ligase subunit beta -
  NO220_RS12170 (NO220_12170) glyQ 2506591..2507478 (-) 888 WP_003152984.1 glycine--tRNA ligase subunit alpha -
  NO220_RS12175 (NO220_12175) recO 2507780..2508547 (-) 768 WP_003152982.1 DNA repair protein RecO Machinery gene
  NO220_RS12180 (NO220_12180) - 2508584..2508727 (-) 144 WP_007408286.1 YqzL family protein -
  NO220_RS12185 (NO220_12185) era 2508883..2509788 (-) 906 WP_003152978.1 GTPase Era -
  NO220_RS12190 (NO220_12190) - 2509769..2510179 (-) 411 WP_003152976.1 cytidine deaminase -
  NO220_RS12195 (NO220_12195) - 2510303..2510677 (-) 375 WP_003152974.1 diacylglycerol kinase family protein -
  NO220_RS12200 (NO220_12200) ybeY 2510658..2511131 (-) 474 WP_003152973.1 rRNA maturation RNase YbeY -
  NO220_RS12205 (NO220_12205) - 2511132..2513264 (-) 2133 WP_003152972.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29264.86 Da        Isoelectric Point: 8.3099

>NTDB_id=711443 NO220_RS12175 WP_003152982.1 2507780..2508547(-) (recO) [Bacillus amyloliquefaciens strain B408]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGARKSASRLSAVSQPFLYGSFLMQRTSGLGTLQQGEMILSMRT
IREDLFLTAYAAFIAELTDKGTEEKKPNPYLFELILESFKRLNDGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSGEG
TFHFSIRDNGFICHRCFEKDPYRVPISPQTARLLRLFYYFDIGRLGSVSLKQETKNEIKQVIDLYYEEYSGLYLKSKRFL
DQMESMKNLLDENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=711443 NO220_RS12175 WP_003152982.1 2507780..2508547(-) (recO) [Bacillus amyloliquefaciens strain B408]
ATGCTGACTAAATGTGAAGGAATCGTGCTCCGTACGAATGACTACGGGGAGACGAATAAAATCGTGACATTGCTGACGAG
GGAACACGGCAAAATCGGCGTCATGGCAAGGGGCGCCAGGAAGTCAGCCAGCCGGCTGTCAGCAGTCAGCCAGCCGTTTT
TGTACGGGTCATTTTTAATGCAAAGAACATCCGGACTCGGCACGCTCCAGCAGGGTGAAATGATTCTCAGCATGAGAACG
ATCAGAGAAGATTTATTTTTGACGGCGTATGCCGCTTTTATCGCTGAACTCACTGACAAAGGAACGGAAGAGAAGAAACC
GAACCCCTATTTATTCGAATTGATTCTCGAATCCTTTAAGAGGCTGAATGACGGGACTGACCCGGATGTGATCACATTCA
TCGTGCAGATGAAAATGCTCGGCGTCATGGGGCTGTATCCGGAGCTGAATCATTGTGTCCACTGCAAAAGCGGGGAAGGG
ACGTTTCATTTTTCGATAAGAGATAACGGTTTTATCTGCCACCGCTGCTTTGAAAAGGATCCGTACAGAGTTCCGATTTC
GCCGCAAACGGCAAGGCTGCTAAGGCTTTTTTATTACTTTGATATCGGAAGGCTCGGCAGCGTCTCGTTAAAACAGGAAA
CCAAAAACGAAATTAAACAGGTCATTGATCTGTATTACGAGGAATATTCAGGACTTTATTTAAAGTCAAAACGGTTTTTA
GACCAGATGGAAAGCATGAAGAACCTTTTGGATGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-237)

Recombination protein O N terminal (RecO_N)

(1-75)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

89.412

100

0.894