Detailed information    

insolico Bioinformatically predicted

Overview

Name   recN   Type   Machinery gene
Locus tag   POF61_RS03055 Genome accession   NZ_CP116709
Coordinates   546299..547957 (+) Length   552 a.a.
NCBI ID   WP_000923615.1    Uniprot ID   -
Organism   Streptococcus agalactiae strain CNCTC 10/84 sCas9revertant     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 541299..552957
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  POF61_RS03025 (POF61_03025) - 541640..542476 (+) 837 WP_000634979.1 NAD(P)H-hydrate dehydratase -
  POF61_RS03030 (POF61_03030) xseA 542602..543942 (+) 1341 WP_001286948.1 exodeoxyribonuclease VII large subunit -
  POF61_RS03035 (POF61_03035) - 543920..544135 (+) 216 WP_001280898.1 exodeoxyribonuclease VII small subunit -
  POF61_RS03040 (POF61_03040) - 544135..545007 (+) 873 WP_000256279.1 polyprenyl synthetase family protein -
  POF61_RS03045 (POF61_03045) - 545000..545827 (+) 828 WP_001041037.1 TlyA family rRNA (cytidine-2'-O)-methyltransferase -
  POF61_RS03050 (POF61_03050) - 545814..546287 (+) 474 WP_000747940.1 ArgR family transcriptional regulator -
  POF61_RS03055 (POF61_03055) recN 546299..547957 (+) 1659 WP_000923615.1 DNA repair protein RecN Machinery gene
  POF61_RS03060 (POF61_03060) - 548070..548906 (+) 837 WP_000034835.1 DegV family protein -
  POF61_RS03065 (POF61_03065) - 548899..549738 (+) 840 WP_001035227.1 SGNH/GDSL hydrolase family protein -
  POF61_RS03070 (POF61_03070) - 549713..550315 (+) 603 WP_000806954.1 YpmS family protein -
  POF61_RS03075 (POF61_03075) - 550418..550693 (+) 276 WP_001284634.1 HU family DNA-binding protein -
  POF61_RS03080 (POF61_03080) - 550781..551923 (-) 1143 WP_000269474.1 site-specific integrase -
  POF61_RS03085 (POF61_03085) - 552038..552589 (-) 552 WP_000353929.1 hypothetical protein -

Sequence

Protein


Download         Length: 552 a.a.        Molecular weight: 62599.88 Da        Isoelectric Point: 4.5181

>NTDB_id=711342 POF61_RS03055 WP_000923615.1 546299..547957(+) (recN) [Streptococcus agalactiae strain CNCTC 10/84 sCas9revertant]
MLLEISIKNFAIIEEISLNFETGMTVLTGETGAGKSIIIDAMNMMLGARASVEVIRHGVNKAEIEGFFSVEKNQSLVQLL
EENGIELADELIIRREIFQNGRSVSRINGQMVNLSTLKAVGHYLVDIHGQHDQEELMKPNMHILMLDEFGNTEFNVIKER
YQSLFDAYRQLRKRVLDKQKNEQENKSRIEMLEFQIAEIESVALKSDEDQTLLKQRDKLMNHKNIADTLTNAYLMLDNEE
FSSLSNVRSAMNDLMALEEFDREYKDLSTNLSEAYYVIEEVTKRLGDVIDDLDFDAGLLQEIENRLDVINTITRKYGGDV
NDVLDYFDNITKEYSLLTGSEESSDALEKELKILEHDLIESANQLSLERHKLAKQLENEIKQELTELYMEKADFQVQFTK
GKFNKEGNEIVEFYISTNPGEGFKPLVKVASGGELSRLMLAIKSAFSRKEDKTSIVFDEVDTGVSGRVAQAIAQKIHKIG
SHGQVLAISHLAQVIAIADYQYFIEKISSDSSTVSTVRLLSYEERVEEIAKMLAGNNVTETARTQAKELLDS

Nucleotide


Download         Length: 1659 bp        

>NTDB_id=711342 POF61_RS03055 WP_000923615.1 546299..547957(+) (recN) [Streptococcus agalactiae strain CNCTC 10/84 sCas9revertant]
ATGCTTTTAGAAATTTCTATTAAGAATTTCGCTATTATCGAAGAAATTTCACTTAATTTTGAAACAGGAATGACTGTTTT
AACTGGTGAGACTGGTGCAGGGAAATCTATCATTATTGATGCTATGAATATGATGTTAGGAGCCCGTGCTAGTGTTGAAG
TGATTCGCCATGGTGTTAACAAAGCAGAAATTGAAGGATTTTTCTCTGTTGAAAAAAATCAATCATTAGTCCAATTATTG
GAAGAAAATGGCATTGAATTAGCAGATGAATTAATTATTCGCCGAGAAATCTTTCAAAATGGGCGCAGCGTTAGTCGAAT
TAATGGGCAAATGGTGAATTTGTCAACACTAAAAGCTGTGGGGCATTATTTGGTAGACATTCATGGTCAGCATGACCAAG
AGGAACTAATGAAACCTAACATGCACATTCTGATGTTAGATGAATTTGGTAATACAGAATTTAATGTCATAAAAGAACGT
TATCAAAGTCTTTTTGATGCTTATCGTCAGCTTCGTAAACGCGTATTGGATAAGCAAAAAAATGAACAAGAGAATAAATC
ACGTATTGAAATGCTAGAATTTCAAATAGCAGAAATTGAGTCTGTAGCCCTTAAATCAGATGAAGACCAAACGCTACTCA
AGCAACGTGATAAATTAATGAACCATAAGAATATTGCAGATACTTTGACAAATGCATATCTTATGTTAGATAACGAAGAG
TTTTCAAGTTTATCGAATGTTCGTTCTGCAATGAATGACCTTATGGCTTTAGAAGAATTTGATCGAGAATATAAAGATCT
TTCCACCAATCTTTCAGAAGCTTACTACGTTATTGAAGAAGTTACTAAACGTTTAGGTGACGTTATCGATGATTTAGATT
TTGACGCTGGTTTACTACAAGAAATTGAAAATAGACTAGATGTTATTAACACAATAACGCGAAAATATGGCGGTGATGTG
AATGATGTTTTAGACTATTTTGATAACATTACTAAAGAATATAGTCTATTGACAGGTAGTGAAGAGTCCTCAGATGCTTT
AGAAAAAGAATTAAAGATTTTAGAACACGATTTGATTGAATCAGCAAATCAATTAAGTTTAGAGCGTCACAAGCTAGCTA
AACAATTGGAAAATGAAATCAAACAAGAATTAACAGAGCTTTATATGGAGAAAGCTGATTTCCAAGTTCAATTTACAAAA
GGAAAATTTAATAAAGAAGGGAACGAAATCGTTGAATTTTATATTTCAACGAATCCCGGAGAGGGCTTTAAGCCATTGGT
AAAAGTGGCTTCAGGAGGAGAGTTATCTCGATTGATGTTAGCTATTAAATCTGCTTTTTCACGTAAGGAAGATAAAACTT
CCATTGTATTTGATGAGGTGGATACAGGAGTTTCTGGTCGCGTGGCCCAGGCTATCGCTCAAAAAATTCACAAGATTGGT
AGTCATGGACAAGTTTTAGCAATTTCTCATTTAGCTCAGGTTATTGCAATAGCGGATTACCAATATTTTATTGAAAAAAT
TTCCAGTGACTCTTCAACAGTTTCAACGGTAAGGCTTTTAAGCTATGAAGAACGTGTAGAAGAAATCGCTAAAATGTTGG
CAGGAAACAATGTAACTGAGACTGCACGTACTCAAGCTAAAGAATTACTTGATAGTTAG

Domains

Predicted by InterProScan.

RecF/RecN/SMC N terminal domain (SMC_N)

(2-506)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recN Bacillus subtilis subsp. subtilis str. 168

43.386

100

0.446