Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   POF63_RS06345 Genome accession   NZ_CP116708
Coordinates   1219808..1222006 (-) Length   732 a.a.
NCBI ID   WP_000622237.1    Uniprot ID   Q8DZA1
Organism   Streptococcus agalactiae strain CNCTC 10/84 sCas9     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1214808..1227006
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  POF63_RS06325 (POF63_06325) - 1214866..1215549 (-) 684 WP_000365679.1 tRNA (adenine(22)-N(1))-methyltransferase TrmK -
  POF63_RS06330 (POF63_06330) - 1215653..1216333 (-) 681 WP_000221657.1 DnaD domain-containing protein -
  POF63_RS06335 (POF63_06335) - 1216451..1216969 (-) 519 WP_000365343.1 adenine phosphoribosyltransferase -
  POF63_RS06340 (POF63_06340) - 1217092..1219656 (-) 2565 WP_001247520.1 GBS Bsp-like repeat-containing protein -
  POF63_RS06345 (POF63_06345) recJ 1219808..1222006 (-) 2199 WP_000622237.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  POF63_RS06350 (POF63_06350) - 1222003..1222764 (-) 762 WP_000124583.1 SDR family oxidoreductase -
  POF63_RS06355 (POF63_06355) rnz 1222766..1223695 (-) 930 WP_000405386.1 ribonuclease Z -
  POF63_RS06360 (POF63_06360) - 1223737..1224384 (-) 648 WP_000568323.1 cystathionine beta-lyase -
  POF63_RS06365 (POF63_06365) hflX 1224377..1225615 (-) 1239 WP_000573348.1 GTPase HflX -
  POF63_RS06370 (POF63_06370) miaA 1225706..1226596 (-) 891 WP_001226482.1 tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA -
  POF63_RS06375 (POF63_06375) - 1226707..1226883 (+) 177 WP_000239229.1 DUF3042 family protein -

Sequence

Protein


Download         Length: 732 a.a.        Molecular weight: 82278.94 Da        Isoelectric Point: 4.5538

>NTDB_id=711267 POF63_RS06345 WP_000622237.1 1219808..1222006(-) (recJ) [Streptococcus agalactiae strain CNCTC 10/84 sCas9]
MISAKYSWVLNNQKPDAGFFEASKKEKISEAVASLIYSRGIKTSAELHHFLQTNLENLHDPYLLNDMDKAVNRIRRAIEN
NETILVYGDYDADGMTSASIMKEALDMMGAEVQVYLPNRFTDGYGPNQSVYKYFIEQQDVSLIITVDNGVAGHEAITYAQ
NQGVDVVVTDHHSMPADLPCAYAIIHPEHPDANYPFPYLAGCGVAFKVACALLETIPTEMLDLVAIGTIADMVSLTDENR
IMVKAGLEVMKDSERIGLQELISLSNIDLKTLNEETIGFKIAPQLNALGRLDDPNPAIELLTGFDDEESQAIAQMIDQKN
EERKEIVQTIFDQAMQMLDQTKPVQVLAKENWHPGVLGIVAGRILERTGQPVIVLNIEDGIAKGSARSVEALDIFQAFDQ
HRELFIAFGGHSGAAGMTLEESKVGDLSQVLCDYISKKQLDMSQKKTLTIDSELRFDELSLDTVRDFEKLAPFGMDNKKP
VFLLKDFKVSQARVMGQNGAHLKLKLEQDGQALDLVAFNMGSQLQEFQQAQHLELAVTLSVNQWNGATTLQLMLEDARVD
GIQLFDIRSKASSLPHGVPILSQEEQSKEVILLTVPDHPQELKQMTQGKQFDAIYFKNEIPKNYFISGYGTRDQFASLYK
TIYQFPEFDVRYKLKELSSYLHIPDILLIKMIQIFEELHFVTITEGIMTVNKEAEKRDISESQIYQELKETVKFQELMAL
GTPKEIYDFMMS

Nucleotide


Download         Length: 2199 bp        

>NTDB_id=711267 POF63_RS06345 WP_000622237.1 1219808..1222006(-) (recJ) [Streptococcus agalactiae strain CNCTC 10/84 sCas9]
ATGATATCTGCTAAGTATAGTTGGGTCCTCAATAACCAAAAGCCAGACGCTGGCTTTTTTGAGGCTTCAAAAAAAGAAAA
GATAAGTGAAGCGGTGGCTTCCTTAATTTATTCGCGAGGTATTAAGACATCAGCGGAATTACATCATTTTTTACAAACTA
ATTTGGAAAATCTCCATGATCCTTATTTGTTAAATGATATGGATAAAGCTGTTAATCGTATTAGACGAGCTATTGAAAAT
AATGAAACTATTTTAGTTTATGGAGACTATGATGCTGATGGAATGACATCAGCATCAATCATGAAAGAAGCATTAGATAT
GATGGGAGCTGAAGTCCAGGTTTATCTCCCAAATCGCTTTACAGATGGTTACGGTCCCAATCAAAGTGTTTATAAATATT
TTATTGAACAACAAGATGTTTCATTAATCATTACGGTTGATAATGGTGTGGCAGGGCATGAAGCAATTACATATGCTCAA
AATCAAGGTGTGGACGTGGTAGTTACTGATCATCACAGTATGCCAGCAGATTTACCTTGTGCTTATGCTATTATTCACCC
AGAACATCCTGATGCTAATTATCCATTTCCTTATTTAGCAGGGTGTGGTGTTGCTTTCAAAGTAGCTTGTGCTCTCTTAG
AAACAATCCCGACAGAAATGTTGGATTTGGTTGCTATTGGGACGATTGCAGATATGGTTAGCCTAACTGATGAAAATCGT
ATCATGGTAAAAGCTGGACTTGAAGTTATGAAAGATAGTGAAAGAATAGGCTTACAGGAGCTAATATCTTTATCAAATAT
TGATTTAAAAACACTAAATGAAGAAACTATTGGATTTAAAATTGCTCCACAATTAAACGCTTTAGGAAGACTTGATGACC
CTAATCCAGCTATTGAATTACTAACAGGGTTTGATGATGAAGAATCTCAAGCAATAGCCCAAATGATTGATCAAAAAAAT
GAAGAGCGCAAAGAAATCGTTCAAACTATCTTTGACCAAGCTATGCAAATGCTTGATCAAACAAAGCCTGTACAAGTTTT
AGCAAAAGAAAATTGGCATCCAGGGGTTTTAGGTATTGTGGCGGGTCGTATTTTAGAGAGAACTGGACAACCTGTTATTG
TTTTGAATATTGAAGATGGTATTGCTAAAGGAAGTGCCAGAAGTGTAGAAGCACTTGATATTTTTCAAGCTTTTGACCAG
CATCGAGAATTATTCATTGCTTTTGGAGGTCACAGCGGAGCAGCAGGCATGACTTTAGAGGAAAGTAAAGTAGGTGATCT
TTCTCAAGTTTTATGTGACTATATCTCTAAAAAACAATTGGACATGAGTCAGAAAAAAACTCTGACAATTGATAGTGAGC
TACGTTTTGACGAACTTAGTTTAGACACTGTTAGAGATTTTGAAAAGTTAGCGCCATTTGGAATGGATAATAAAAAACCA
GTCTTTTTGTTAAAAGATTTTAAAGTGAGTCAAGCCAGAGTCATGGGGCAAAACGGTGCTCATCTGAAATTAAAACTAGA
ACAAGATGGACAAGCACTTGATTTGGTAGCATTTAATATGGGGAGTCAACTACAAGAGTTCCAACAAGCACAACATTTGG
AGTTAGCTGTCACTTTATCTGTTAATCAATGGAATGGTGCCACAACCCTACAATTAATGTTGGAGGATGCGCGTGTAGAC
GGTATTCAATTATTTGATATTAGAAGCAAAGCAAGTAGTTTACCTCATGGAGTGCCAATCCTGTCTCAAGAAGAGCAGAG
CAAAGAAGTTATTCTTTTGACTGTTCCCGATCATCCACAAGAATTAAAACAAATGACTCAAGGTAAACAGTTTGATGCCA
TCTATTTTAAAAATGAGATACCAAAAAATTATTTTATATCTGGTTATGGTACACGTGATCAGTTTGCTAGTTTATATAAA
ACAATATATCAATTCCCAGAATTTGACGTTCGTTATAAGTTAAAAGAGCTCAGTTCCTACCTTCATATCCCTGATATTTT
ATTAATTAAAATGATTCAAATTTTTGAAGAACTACATTTTGTTACGATTACAGAAGGGATTATGACAGTCAATAAAGAAG
CAGAAAAGCGAGATATCTCTGAGAGTCAAATTTATCAAGAATTAAAAGAGACAGTTAAGTTTCAAGAATTAATGGCTTTA
GGGACACCTAAAGAAATATATGATTTTATGATGAGTTGA

Domains

Predicted by InterProScan.

RecJ OB domain (RecJ_OB)

(450-556)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(583-726)

DHH family (DHH)

(83-228)

DHHA1 domain (DHHA1)

(317-433)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8DZA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Streptococcus pneumoniae strain D39V

64.858

100

0.653
  recJ Bacillus subtilis subsp. subtilis str. 168

35.881

100

0.378