Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   POF65_RS06580 Genome accession   NZ_CP116706
Coordinates   1268652..1270850 (-) Length   732 a.a.
NCBI ID   WP_000622234.1    Uniprot ID   -
Organism   Streptococcus agalactiae strain A909 sCas9     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1263652..1275850
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  POF65_RS06560 (POF65_06560) - 1263710..1264393 (-) 684 WP_000365678.1 tRNA (adenine(22)-N(1))-methyltransferase TrmK -
  POF65_RS06565 (POF65_06565) - 1264497..1265177 (-) 681 WP_000221659.1 DnaD domain-containing protein -
  POF65_RS06570 (POF65_06570) - 1265295..1265813 (-) 519 WP_000365344.1 adenine phosphoribosyltransferase -
  POF65_RS06575 (POF65_06575) - 1265936..1268500 (-) 2565 WP_001247516.1 GBS Bsp-like repeat-containing protein -
  POF65_RS06580 (POF65_06580) recJ 1268652..1270850 (-) 2199 WP_000622234.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  POF65_RS06585 (POF65_06585) - 1270847..1271608 (-) 762 WP_000124582.1 SDR family oxidoreductase -
  POF65_RS06590 (POF65_06590) rnz 1271610..1272539 (-) 930 WP_000405388.1 ribonuclease Z -
  POF65_RS06595 (POF65_06595) - 1272581..1273228 (-) 648 WP_000568316.1 cystathionine beta-lyase -
  POF65_RS06600 (POF65_06600) hflX 1273221..1274459 (-) 1239 WP_000573349.1 GTPase HflX -
  POF65_RS06605 (POF65_06605) miaA 1274550..1275440 (-) 891 WP_001226485.1 tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA -
  POF65_RS06610 (POF65_06610) - 1275551..1275727 (+) 177 WP_000239229.1 DUF3042 family protein -

Sequence

Protein


Download         Length: 732 a.a.        Molecular weight: 82161.84 Da        Isoelectric Point: 4.5556

>NTDB_id=711049 POF65_RS06580 WP_000622234.1 1268652..1270850(-) (recJ) [Streptococcus agalactiae strain A909 sCas9]
MISAKYSWVLNNQKPDAGFFEASKKEKISEAVASLIYSRGIKTSAELHHFLQTNLENLHDPYLLNDMDKAVNRIRRAIEN
NETILVYGDYDADGMTSASIMKEALDMMGAEVQVYLPNRFTDGYGPNESVYKYFIEQQGVSLIITVDNGVAGHEAIAYAQ
NQGVDVVVTDHHSMPADLPCAYAIIHPEHPDANYPFPYLAGCGVAFKVACALLETIPTEMLDLVAIGTIADMVSLTDENR
IMVKAGLEVMKDSERIGLQELISLSNIDLKTLNEETIGFKIAPQLNALGRLDDPNPAIELLTGFDDEESQAIAQMIDQKN
EERKEIVQTIFDQAMQMLDQTKPVQVLAKENWHPGVLGIVAGRILERTGQPVIVLNIEDGIAKGSARSVEALDIFQAFDQ
HRELFIAFGGHSGAAGMTLEESKVGDLSQVLCDYISKKQLDMGQKKTLTIDSELRFDELSLDTVRDFEKLAPFGMDNKKP
VFLLKDFKVSQARVMGQNGAHLKLKLEQDGQALDLVAFNMGSQLQEFQQAQHLELAVTLSVNQWNGATTLQLMLEDARVD
GIQLFDIRSKASSLPHGVPILSQEEQSKEVILLTVPDHPQELKQMTQGKQFDAIYFKNEIPKNYFISGYGTRDQFASLYK
TIYQFPEFDVRYKLKELSSYLHIPDILLIKMIQIFEELHFVTITEGIMTVNKEAEKRDISESQIYQELKETVKFQELMAL
GTPKEIYDFMMS

Nucleotide


Download         Length: 2199 bp        

>NTDB_id=711049 POF65_RS06580 WP_000622234.1 1268652..1270850(-) (recJ) [Streptococcus agalactiae strain A909 sCas9]
ATGATATCTGCTAAGTATAGTTGGGTCCTCAATAACCAAAAGCCAGACGCTGGCTTTTTTGAGGCTTCAAAAAAAGAAAA
GATAAGTGAAGCGGTGGCTTCCTTAATTTATTCGCGAGGTATTAAGACATCAGCGGAATTACATCATTTTTTACAAACTA
ATTTGGAAAATCTCCATGATCCTTATTTGTTAAATGATATGGATAAAGCTGTTAATCGTATTAGACGAGCTATTGAAAAT
AATGAAACTATTTTAGTTTATGGAGACTATGATGCTGATGGAATGACATCAGCATCAATCATGAAAGAAGCTTTAGATAT
GATGGGAGCTGAAGTCCAGGTTTATCTCCCAAATCGCTTTACAGATGGTTACGGTCCCAATGAAAGTGTTTATAAATATT
TTATTGAACAACAAGGTGTTTCATTAATCATTACGGTTGATAATGGTGTGGCGGGGCATGAAGCAATTGCATATGCTCAA
AATCAAGGTGTGGACGTGGTAGTTACTGATCATCACAGTATGCCAGCAGATTTACCTTGTGCTTATGCTATTATTCACCC
AGAACATCCTGATGCTAATTATCCATTTCCTTATTTAGCAGGGTGTGGTGTTGCTTTTAAAGTAGCTTGTGCTCTCTTAG
AAACAATCCCGACAGAAATGTTGGATTTGGTTGCTATTGGGACGATTGCAGATATGGTTAGCCTAACTGATGAAAATCGT
ATCATGGTAAAAGCTGGACTTGAAGTTATGAAAGATAGTGAAAGAATAGGCTTACAGGAGCTAATATCTTTATCAAATAT
TGATTTAAAAACACTAAATGAAGAAACTATTGGATTTAAAATTGCTCCACAATTAAACGCTTTAGGAAGACTTGATGACC
CTAATCCAGCTATTGAATTACTGACAGGGTTTGATGATGAAGAATCTCAAGCAATAGCCCAAATGATTGATCAAAAAAAT
GAAGAGCGCAAAGAAATCGTTCAAACTATCTTTGACCAAGCTATGCAAATGCTTGATCAAACAAAGCCTGTACAAGTTTT
AGCAAAAGAAAATTGGCATCCAGGGGTTTTAGGTATTGTGGCGGGTCGTATTTTAGAGAGAACTGGACAACCTGTTATTG
TTTTGAATATTGAAGATGGTATTGCTAAAGGAAGTGCCAGAAGTGTAGAAGCACTTGATATTTTTCAAGCTTTTGACCAG
CATCGAGAATTATTCATTGCTTTTGGAGGTCACAGCGGAGCAGCAGGCATGACTTTAGAGGAAAGTAAGGTGGGGGATCT
TTCTCAAGTTTTATGTGACTATATCTCTAAAAAACAATTGGACATGGGTCAGAAAAAAACTCTGACAATTGATAGTGAGC
TACGTTTTGACGAACTTAGTTTAGACACTGTTAGAGATTTTGAAAAGTTAGCGCCATTTGGAATGGATAATAAAAAGCCA
GTCTTTTTGTTAAAAGATTTTAAAGTGAGTCAAGCTAGAGTCATGGGGCAAAACGGTGCTCATCTGAAATTAAAACTAGA
ACAAGATGGACAAGCACTTGATTTGGTAGCATTTAATATGGGGAGTCAACTACAAGAGTTCCAACAAGCACAACATTTGG
AGTTAGCTGTCACTTTATCTGTTAATCAATGGAATGGTGCCACAACCCTACAATTAATGTTGGAGGATGCGCGTGTAGAT
GGTATTCAATTATTTGATATTAGAAGCAAAGCAAGTAGCTTACCTCATGGAGTGCCAATCCTATCTCAAGAAGAGCAGAG
CAAAGAAGTTATTCTTTTGACTGTTCCCGATCATCCACAAGAATTAAAACAAATGACTCAAGGTAAACAGTTTGATGCCA
TCTATTTTAAAAATGAGATACCAAAAAATTATTTTATATCTGGTTATGGTACACGTGATCAGTTTGCTAGTTTATATAAA
ACAATATATCAATTCCCAGAATTTGACGTTCGTTATAAGTTAAAAGAGCTCAGTTCCTACCTTCATATTCCTGATATTTT
ATTAATTAAAATGATTCAAATTTTTGAAGAACTACATTTTGTTACGATTACAGAAGGGATTATGACAGTCAATAAAGAAG
CAGAAAAGCGAGATATCTCTGAGAGTCAAATTTATCAAGAATTAAAAGAGACAGTTAAGTTTCAAGAATTAATGGCTTTA
GGGACACCTAAAGAAATATATGATTTTATGATGAGTTGA

Domains

Predicted by InterProScan.

RecJ OB domain (RecJ_OB)

(450-556)

DHHA1 domain (DHHA1)

(317-433)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(583-726)

DHH family (DHH)

(83-228)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Streptococcus pneumoniae strain D39V

65.265

100

0.657
  recJ Bacillus subtilis subsp. subtilis str. 168

36.14

100

0.381