Detailed information    

insolico Bioinformatically predicted

Overview

Name   recN   Type   Machinery gene
Locus tag   POF65_RS03020 Genome accession   NZ_CP116706
Coordinates   544482..546140 (+) Length   552 a.a.
NCBI ID   WP_000923619.1    Uniprot ID   -
Organism   Streptococcus agalactiae strain A909 sCas9     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 539482..551140
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  POF65_RS02990 (POF65_02990) - 539823..540659 (+) 837 WP_000634981.1 NAD(P)H-hydrate dehydratase -
  POF65_RS02995 (POF65_02995) xseA 540785..542125 (+) 1341 WP_001286939.1 exodeoxyribonuclease VII large subunit -
  POF65_RS03000 (POF65_03000) - 542103..542318 (+) 216 WP_001280900.1 exodeoxyribonuclease VII small subunit -
  POF65_RS03005 (POF65_03005) - 542318..543190 (+) 873 WP_000256279.1 polyprenyl synthetase family protein -
  POF65_RS03010 (POF65_03010) - 543183..544010 (+) 828 WP_001041040.1 TlyA family rRNA (cytidine-2'-O)-methyltransferase -
  POF65_RS03015 (POF65_03015) - 543997..544470 (+) 474 WP_000747943.1 ArgR family transcriptional regulator -
  POF65_RS03020 (POF65_03020) recN 544482..546140 (+) 1659 WP_000923619.1 DNA repair protein RecN Machinery gene
  POF65_RS03025 (POF65_03025) - 546253..547089 (+) 837 WP_000034838.1 DegV family protein -
  POF65_RS03030 (POF65_03030) - 547082..547921 (+) 840 WP_001035227.1 SGNH/GDSL hydrolase family protein -
  POF65_RS03035 (POF65_03035) - 547896..548498 (+) 603 WP_000806954.1 YpmS family protein -
  POF65_RS03040 (POF65_03040) - 548601..548876 (+) 276 WP_001284634.1 HU family DNA-binding protein -
  POF65_RS03045 (POF65_03045) - 548964..550106 (-) 1143 WP_000269476.1 site-specific integrase -
  POF65_RS03050 (POF65_03050) - 550234..550443 (-) 210 WP_000442169.1 TM2 domain-containing protein -
  POF65_RS03055 (POF65_03055) - 550495..550875 (-) 381 WP_000700104.1 ImmA/IrrE family metallo-endopeptidase -

Sequence

Protein


Download         Length: 552 a.a.        Molecular weight: 62515.76 Da        Isoelectric Point: 4.5358

>NTDB_id=711017 POF65_RS03020 WP_000923619.1 544482..546140(+) (recN) [Streptococcus agalactiae strain A909 sCas9]
MLLEISIKNFAIIEEISLNFETGMTVLTGETGAGKSIIIDAMNMMLGSRASVEVIRHGANKAEIEGFFSVEKNQSLVQLL
EENGIELADELIIRREIFQNGRSVSRINGQMVNLSTLKAVGHYLVDIHGQHDQEELMKPNMHILMLDEFGNTEFNVIKER
YQSLFDAYRQLRKRVLDKQKNEQENKSRIEMLEFQIAEIESVALKSDEDQTLLKQRDKLMNHKNIADTLTNAYLMLDNEE
FSSLSNVRSAMNDLMALEEFDREYKDLSTNLSEAYYVIEEVTKRLGDVIDDLDFDAGLLQEIENRLDVINTITRKYGGDV
NDVLDYFDNITKEYSLLTGSEESSDALEKELKILEHDLIESANQLSLERHKLAKQLENEIKQELTELYMEKADFQVQFTK
GKFNKEGNEIVEFYISTNPGEGFKPLVKVASGGELSRLMLAIKSAFSRKEDKTSIVFDEVDTGVSGRVAQAIAQKIHKIG
SHGQVLAISHLAQVIAIADYQYFIEKISSDSSTVSTVRLLSYEERVEEIAKMLAGNNVTDTARTQAKELLGS

Nucleotide


Download         Length: 1659 bp        

>NTDB_id=711017 POF65_RS03020 WP_000923619.1 544482..546140(+) (recN) [Streptococcus agalactiae strain A909 sCas9]
ATGCTTTTAGAAATTTCTATTAAGAATTTCGCTATTATCGAAGAAATTTCACTTAATTTTGAAACAGGAATGACTGTTTT
AACTGGTGAGACTGGTGCAGGGAAATCTATCATTATTGATGCTATGAATATGATGTTAGGATCCCGTGCTAGCGTTGAAG
TGATTCGCCATGGTGCTAACAAAGCAGAAATTGAAGGATTTTTCTCTGTTGAAAAAAATCAATCATTAGTCCAATTATTG
GAAGAAAATGGCATTGAATTAGCAGATGAATTAATTATTCGCCGAGAAATCTTTCAAAATGGGCGCAGCGTTAGTCGAAT
TAATGGGCAAATGGTGAATTTGTCAACACTAAAAGCTGTGGGGCATTATTTGGTAGACATTCATGGTCAGCATGACCAAG
AGGAACTAATGAAACCTAACATGCACATTCTGATGTTAGATGAATTTGGTAATACAGAATTTAATGTCATAAAAGAACGT
TATCAAAGTCTTTTTGATGCTTATCGTCAGCTTCGTAAACGCGTATTGGATAAGCAAAAAAATGAACAAGAGAATAAATC
ACGTATTGAAATGCTAGAATTTCAAATAGCAGAAATTGAGTCTGTAGCCCTTAAATCAGATGAAGACCAAACGCTACTCA
AGCAACGTGATAAATTAATGAATCATAAGAATATTGCAGATACTTTGACAAATGCATATCTTATGTTAGATAACGAAGAG
TTTTCAAGTTTATCGAATGTTCGTTCTGCAATGAATGACCTTATGGCTTTAGAAGAATTTGATCGAGAATATAAAGATCT
TTCCACCAATCTTTCAGAAGCTTACTACGTTATTGAAGAAGTTACTAAACGTTTAGGTGACGTTATCGATGATTTAGATT
TTGACGCTGGTTTACTACAAGAAATTGAAAATAGACTAGATGTTATTAACACAATAACGCGAAAATATGGCGGTGATGTG
AATGATGTTTTAGACTATTTTGATAACATTACTAAAGAATATAGTCTATTGACAGGTAGTGAAGAGTCCTCAGATGCTTT
AGAAAAAGAATTAAAGATTTTAGAACACGATTTGATTGAATCAGCAAATCAATTAAGTTTAGAGCGCCATAAGCTAGCTA
AACAATTGGAAAATGAAATCAAACAAGAATTAACAGAGCTTTATATGGAGAAAGCTGATTTCCAAGTTCAATTTACAAAA
GGAAAATTTAATAAAGAAGGGAACGAAATCGTTGAATTTTATATTTCAACGAATCCCGGAGAGGGCTTTAAGCCATTGGT
AAAAGTGGCTTCAGGAGGAGAGTTATCTCGATTGATGTTAGCTATTAAATCTGCTTTTTCACGTAAGGAAGATAAAACTT
CCATTGTATTTGATGAGGTGGATACAGGAGTTTCTGGTCGCGTGGCCCAGGCTATCGCTCAAAAAATTCACAAGATTGGT
AGTCATGGACAAGTTTTAGCAATTTCTCATTTAGCTCAGGTTATTGCAATAGCGGATTACCAATATTTTATTGAAAAAAT
TTCCAGTGACTCTTCAACAGTTTCAACGGTAAGGCTTTTAAGCTATGAAGAACGTGTAGAAGAAATCGCTAAAATGTTGG
CAGGAAACAATGTAACAGACACTGCACGTACTCAAGCTAAAGAATTACTTGGTAGTTAA

Domains

Predicted by InterProScan.

RecF/RecN/SMC N terminal domain (SMC_N)

(2-506)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recN Bacillus subtilis subsp. subtilis str. 168

43.563

100

0.447