Detailed information    

insolico Bioinformatically predicted

Overview

Name   recU   Type   Machinery gene
Locus tag   POF65_RS01855 Genome accession   NZ_CP116706
Coordinates   320085..320684 (-) Length   199 a.a.
NCBI ID   WP_000248792.1    Uniprot ID   Q3K380
Organism   Streptococcus agalactiae strain A909 sCas9     
Function   plasmid transformation; homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 315085..325684
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  POF65_RS01840 (POF65_01840) nadE 315538..316359 (+) 822 WP_000174854.1 ammonia-dependent NAD(+) synthetase -
  POF65_RS01845 (POF65_01845) pepC 316472..317806 (+) 1335 WP_000041114.1 aminopeptidase C -
  POF65_RS01850 (POF65_01850) pbp1a 317852..320098 (-) 2247 WP_000628369.1 penicillin-binding protein PBP1A -
  POF65_RS01855 (POF65_01855) recU 320085..320684 (-) 600 WP_000248792.1 Holliday junction resolvase RecU Machinery gene
  POF65_RS01860 (POF65_01860) - 320759..321277 (+) 519 WP_000843096.1 DUF1273 domain-containing protein -
  POF65_RS01865 (POF65_01865) gpsB 321398..321730 (+) 333 WP_000146544.1 cell division regulator GpsB -
  POF65_RS01875 (POF65_01875) - 322200..323354 (+) 1155 WP_000664851.1 class I SAM-dependent RNA methyltransferase -
  POF65_RS01880 (POF65_01880) - 323367..324830 (+) 1464 WP_001287870.1 cell division site-positioning protein MapZ family protein -
  POF65_RS01885 (POF65_01885) - 325055..325537 (-) 483 WP_000159885.1 S-ribosylhomocysteine lyase -

Sequence

Protein


Download         Length: 199 a.a.        Molecular weight: 23101.69 Da        Isoelectric Point: 9.8314

>NTDB_id=711004 POF65_RS01855 WP_000248792.1 320085..320684(-) (recU) [Streptococcus agalactiae strain A909 sCas9]
MVNYPHQLIRKTTVTKSKKKKIDFANRGMSFEAAINATNDYYLSHELAVIHKKPTPVQIVKVDYPKRSRAKIVEAYFRQA
STTDYSGVYKGYYIDFEAKETRQKTAMPMKNFHAHQIEHMANVLQQKGICFVLLHFSTLKETYLLPANELISFYQIDKGN
KSMPIDYIRKNGFFVKESAFPQVPYLDIIEEKLLGGDYN

Nucleotide


Download         Length: 600 bp        

>NTDB_id=711004 POF65_RS01855 WP_000248792.1 320085..320684(-) (recU) [Streptococcus agalactiae strain A909 sCas9]
ATGGTTAACTATCCACATCAGCTTATTCGGAAAACAACAGTTACAAAATCAAAGAAAAAGAAAATCGATTTTGCCAACCG
TGGTATGTCATTTGAAGCTGCTATCAATGCAACCAATGACTACTATTTGTCTCACGAATTAGCTGTCATTCATAAGAAAC
CAACGCCAGTACAAATCGTCAAGGTAGATTATCCTAAACGTAGTCGTGCTAAGATTGTAGAAGCTTATTTTAGACAAGCT
TCTACTACTGATTATTCTGGTGTTTACAAAGGTTACTATATTGACTTTGAAGCCAAAGAAACCCGGCAGAAAACTGCTAT
GCCTATGAAAAATTTTCATGCTCACCAAATAGAGCACATGGCAAATGTATTACAGCAAAAAGGGATTTGCTTTGTCTTGC
TTCATTTTTCCACACTTAAGGAAACCTATCTACTCCCTGCTAATGAGTTAATTTCATTTTATCAGATTGATAAAGGCAAT
AAATCAATGCCTATTGATTATATCAGAAAAAATGGATTTTTCGTAAAGGAGAGTGCCTTTCCTCAAGTCCCTTACTTAGA
TATTATTGAAGAAAAATTATTAGGCGGTGATTACAATTAA

Domains

Predicted by InterProScan.

Recombination protein U (RecU)

(27-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3K380

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recU Bacillus subtilis subsp. subtilis str. 168

48.223

98.995

0.477