Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   NNG64_RS05685 Genome accession   NZ_CP101610
Coordinates   1077428..1078498 (+) Length   356 a.a.
NCBI ID   WP_045926720.1    Uniprot ID   A0AAI8HN13
Organism   Bacillus siamensis strain WYJ-E14     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1072428..1083498
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NNG64_RS05655 (NNG64_05645) - 1072899..1073144 (-) 246 WP_003153078.1 DUF2626 domain-containing protein -
  NNG64_RS05660 (NNG64_05650) - 1073381..1073761 (+) 381 WP_060964760.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  NNG64_RS05670 (NNG64_05660) - 1073970..1074800 (+) 831 WP_060964761.1 STAS domain-containing protein -
  NNG64_RS05675 (NNG64_05665) - 1074838..1076139 (-) 1302 WP_060964762.1 hemolysin family protein -
  NNG64_RS05680 (NNG64_05670) - 1076285..1077235 (+) 951 WP_060964763.1 magnesium transporter CorA family protein -
  NNG64_RS05685 (NNG64_05675) comGA 1077428..1078498 (+) 1071 WP_045926720.1 competence type IV pilus ATPase ComGA Machinery gene
  NNG64_RS05690 (NNG64_05680) comGB 1078485..1079522 (+) 1038 WP_060964764.1 competence type IV pilus assembly protein ComGB Machinery gene
  NNG64_RS05695 (NNG64_05685) comGC 1079527..1079835 (+) 309 WP_016938966.1 competence type IV pilus major pilin ComGC Machinery gene
  NNG64_RS05700 (NNG64_05690) comGD 1079825..1080262 (+) 438 WP_060964765.1 competence type IV pilus minor pilin ComGD Machinery gene
  NNG64_RS05705 (NNG64_05695) comGE 1080246..1080560 (+) 315 WP_060964766.1 competence type IV pilus minor pilin ComGE Machinery gene
  NNG64_RS05710 (NNG64_05700) comGF 1080472..1080969 (+) 498 WP_235588382.1 competence type IV pilus minor pilin ComGF -
  NNG64_RS05715 (NNG64_05705) comGG 1080970..1081347 (+) 378 WP_060964768.1 competence type IV pilus minor pilin ComGG Machinery gene
  NNG64_RS05720 (NNG64_05710) - 1081404..1081583 (+) 180 WP_022552966.1 YqzE family protein -
  NNG64_RS05725 (NNG64_05715) - 1081624..1081953 (-) 330 WP_016938972.1 DUF3889 domain-containing protein -
  NNG64_RS05730 (NNG64_05720) tapA 1082212..1082883 (+) 672 WP_060964769.1 amyloid fiber anchoring/assembly protein TapA -
  NNG64_RS05735 (NNG64_05725) sipW 1082855..1083439 (+) 585 WP_060964770.1 signal peptidase I SipW -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40409.64 Da        Isoelectric Point: 9.2036

>NTDB_id=710843 NNG64_RS05685 WP_045926720.1 1077428..1078498(+) (comGA) [Bacillus siamensis strain WYJ-E14]
MENIETYSRNIINEAYTARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPSETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFPGAGAALLSFLKHSHGLILFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKAENCSVYCRLYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=710843 NNG64_RS05685 WP_045926720.1 1077428..1078498(+) (comGA) [Bacillus siamensis strain WYJ-E14]
ATGGAGAATATTGAAACATACAGCAGGAACATTATAAACGAGGCCTATACGGCAAGAGCTTCAGATATACACATTGTGCC
GCGTGAAAGGGATGCTTTCATTCATTTTCGCATTGGTCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTTAAGTTTTTATCGGCGATGGATATAGGAGAAAGACGAAAGCCGCAAAACGGATCATTGTCT
CTGCCGCTTCCATCCGAAACCGTTCATTTAAGGATGTCAACCTTACCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCCGGGGGCAGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCCCACGGCCTGATTCTTTTTACCGGTCCGACCGGATCTGGAAAAACGACCACTCTCTATTCGCTTGTCAATTAT
GCAAAACGTCATTTCAACCGGCGGATTGTCACGCTTGAAGATCCCGTAGAAACGAGAGATGAAGATGTTTTACAAGTTCA
AGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGACCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATTGCAGTCCGCGCGGCTATGACGGGGCACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCTATATACAGGCTGCTGGAGTTTGGGGTGAACATGACGGAAATTGAACAAACCGTTATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACGTGTCCGTTTTGCAAAGCGGAAAACTGCTCCGTGTACTGCCGGCTTTACA
GGCAGACGCGCCGTGCGGGAATCTATGAACTGCTTTACGGGAAAAATCTCACCCAATGCTTTCAGGAAGCAAAAGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCCGAAAAGGAATCGCGCTCGGCTATGTCACGACTGACAACTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.303

100

0.823
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379