Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   NNG64_RS05430 Genome accession   NZ_CP101610
Coordinates   1032801..1033568 (+) Length   255 a.a.
NCBI ID   WP_060964730.1    Uniprot ID   A0AAI8HN04
Organism   Bacillus siamensis strain WYJ-E14     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 1027801..1038568
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NNG64_RS05400 (NNG64_05390) - 1028086..1030218 (+) 2133 WP_060964728.1 HD family phosphohydrolase -
  NNG64_RS05405 (NNG64_05395) ybeY 1030219..1030692 (+) 474 WP_003152973.1 rRNA maturation RNase YbeY -
  NNG64_RS05410 (NNG64_05400) - 1030673..1031047 (+) 375 WP_060964729.1 diacylglycerol kinase family protein -
  NNG64_RS05415 (NNG64_05405) - 1031169..1031579 (+) 411 WP_016938918.1 cytidine deaminase -
  NNG64_RS05420 (NNG64_05410) era 1031560..1032465 (+) 906 WP_016938919.1 GTPase Era -
  NNG64_RS05425 (NNG64_05415) - 1032621..1032764 (+) 144 WP_007408286.1 YqzL family protein -
  NNG64_RS05430 (NNG64_05420) recO 1032801..1033568 (+) 768 WP_060964730.1 DNA repair protein RecO Machinery gene
  NNG64_RS05435 (NNG64_05425) glyQ 1033870..1034757 (+) 888 WP_060964731.1 glycine--tRNA ligase subunit alpha -
  NNG64_RS05440 (NNG64_05430) glyS 1034750..1036789 (+) 2040 WP_060964732.1 glycine--tRNA ligase subunit beta -
  NNG64_RS05445 (NNG64_05435) ccpN 1036891..1037529 (+) 639 WP_003152989.1 transcriptional regulator CcpN -
  NNG64_RS05450 (NNG64_05440) - 1037555..1038367 (+) 813 WP_060964733.1 pyruvate, water dikinase regulatory protein -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29344.99 Da        Isoelectric Point: 8.5632

>NTDB_id=710842 NNG64_RS05430 WP_060964730.1 1032801..1033568(+) (recO) [Bacillus siamensis strain WYJ-E14]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGARKSASRLSAVSQPFLYGSFLMQRTSGLGTLQQGEMILSMRT
IREDLFLTAYAAFIAELTDKGTEEKKPNPYLFELILESFKRLNEGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSRDG
TFHFSIRDNGFICHRCFEKDPYKVPISPQTARLLRLFYYFDIGRLGSVSLKHETKNEIKQVIDLYYEEYSGLYLKSKRFL
DQMESMKNLLDENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=710842 NNG64_RS05430 WP_060964730.1 1032801..1033568(+) (recO) [Bacillus siamensis strain WYJ-E14]
ATGCTGACAAAATGTGAAGGAATCGTGCTCCGTACGAATGACTACGGAGAGACGAATAAAATCGTGACATTGCTGACGAG
GGAACACGGCAAAATCGGCGTCATGGCAAGGGGTGCCAGGAAGTCAGCCAGCCGGCTGTCAGCAGTCAGCCAGCCTTTTT
TGTACGGGTCATTTTTAATGCAAAGAACATCCGGACTCGGCACGCTCCAGCAGGGTGAAATGATTCTCAGCATGAGAACG
ATCAGAGAAGACTTGTTTTTGACGGCATATGCCGCTTTTATCGCTGAACTCACTGACAAAGGAACGGAAGAGAAGAAACC
GAACCCCTATTTATTCGAATTGATTCTCGAATCCTTTAAGAGGCTGAATGAGGGGACTGACCCGGACGTGATCACCTTCA
TCGTTCAGATGAAAATGCTTGGCGTCATGGGGCTGTATCCGGAGCTGAATCATTGTGTCCACTGTAAAAGCCGGGACGGG
ACGTTTCATTTTTCGATAAGAGATAACGGTTTTATCTGCCACCGCTGCTTTGAAAAAGATCCGTACAAAGTTCCGATTTC
GCCGCAAACGGCAAGGCTGCTAAGGCTTTTTTATTACTTTGATATCGGAAGGCTCGGCAGCGTCTCGTTAAAGCACGAAA
CCAAAAATGAAATAAAACAGGTCATTGATCTGTATTACGAGGAATATTCAGGGCTTTATTTAAAGTCAAAGCGGTTTTTA
GATCAGATGGAAAGCATGAAGAACCTTTTGGATGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-237)

Recombination protein O N terminal (RecO_N)

(1-75)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

89.804

100

0.898