Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   NNL98_RS01680 Genome accession   NZ_CP101537
Coordinates   326857..328233 (+) Length   458 a.a.
NCBI ID   WP_003549080.1    Uniprot ID   Q5FM36
Organism   Lactobacillus acidophilus strain HN017     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 325878..348663 326857..328233 within 0


Gene organization within MGE regions


Location: 325878..348663
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NNL98_RS01670 (NNL98_01670) - 325878..326174 (-) 297 WP_003549078.1 hypothetical protein -
  NNL98_RS01675 (NNL98_01675) - 326306..326857 (+) 552 WP_011254121.1 dUTP diphosphatase -
  NNL98_RS01680 (NNL98_01680) radA 326857..328233 (+) 1377 WP_003549080.1 DNA repair protein RadA Machinery gene
  NNL98_RS01685 (NNL98_01685) gltX 328311..329810 (+) 1500 WP_003549081.1 glutamate--tRNA ligase -
  NNL98_RS01690 (NNL98_01690) cysS 329908..331341 (+) 1434 WP_003549082.1 cysteine--tRNA ligase -
  NNL98_RS01695 (NNL98_01695) - 331334..331777 (+) 444 WP_003549083.1 Mini-ribonuclease 3 -
  NNL98_RS01700 (NNL98_01700) rlmB 331764..332516 (+) 753 WP_003549084.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  NNL98_RS01705 (NNL98_01705) - 332656..333192 (+) 537 WP_011254124.1 DNA-directed RNA polymerase subunit sigma -
  NNL98_RS01710 (NNL98_01710) - 333291..333509 (+) 219 WP_003549087.1 hypothetical protein -
  NNL98_RS01715 (NNL98_01715) - 333648..334256 (+) 609 WP_003549088.1 hypothetical protein -
  NNL98_RS01720 (NNL98_01720) - 334258..335742 (+) 1485 WP_021721659.1 gluconokinase -
  NNL98_RS01725 (NNL98_01725) - 335739..336620 (-) 882 WP_255788724.1 AraC family transcriptional regulator -
  NNL98_RS01730 (NNL98_01730) - 336743..338485 (-) 1743 WP_003549091.1 1-deoxy-D-xylulose-5-phosphate synthase -
  NNL98_RS01735 (NNL98_01735) rpmG 338673..338822 (+) 150 WP_003549092.1 50S ribosomal protein L33 -
  NNL98_RS01740 (NNL98_01740) secE 338831..338998 (+) 168 WP_003549093.1 preprotein translocase subunit SecE -
  NNL98_RS01745 (NNL98_01745) nusG 339112..339669 (+) 558 WP_003549094.1 transcription termination/antitermination protein NusG -
  NNL98_RS01750 (NNL98_01750) rplK 339796..340221 (+) 426 WP_003549095.1 50S ribosomal protein L11 -
  NNL98_RS01755 (NNL98_01755) rplA 340301..340993 (+) 693 WP_003549096.1 50S ribosomal protein L1 -
  NNL98_RS01760 (NNL98_01760) - 341097..341963 (+) 867 WP_011254125.1 phosphate ABC transporter substrate-binding protein -
  NNL98_RS01765 (NNL98_01765) pstC 341967..342962 (+) 996 WP_003549098.1 phosphate ABC transporter permease subunit PstC -
  NNL98_RS01770 (NNL98_01770) pstA 342965..343852 (+) 888 WP_003549099.1 phosphate ABC transporter permease PstA -
  NNL98_RS01775 (NNL98_01775) pstB 343861..344655 (+) 795 WP_015613297.1 phosphate ABC transporter ATP-binding protein PstB -
  NNL98_RS01780 (NNL98_01780) pstB 344657..345412 (+) 756 WP_003549101.1 phosphate ABC transporter ATP-binding protein PstB -
  NNL98_RS01785 (NNL98_01785) phoU 345429..346106 (+) 678 WP_003549102.1 phosphate signaling complex protein PhoU -
  NNL98_RS01790 (NNL98_01790) - 346289..347470 (+) 1182 WP_021874153.1 AEC family transporter -
  NNL98_RS01795 (NNL98_01795) - 347526..347897 (-) 372 WP_021874154.1 hypothetical protein -
  NNL98_RS01800 (NNL98_01800) rplJ 348151..348663 (+) 513 WP_003549105.1 50S ribosomal protein L10 -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50253.83 Da        Isoelectric Point: 6.9044

>NTDB_id=710481 NNL98_RS01680 WP_003549080.1 326857..328233(+) (radA) [Lactobacillus acidophilus strain HN017]
MARVKTQYKCRSCGYISASYLGRCPNCGAWNQFEKETEEVQKRSTKATASRLIQKTGVNEPVKLDKIKAEKEERISTKSE
ELNRVLGGGIVPGSLVLIGGDPGIGKSTLMLQIMSDLAEKHKVLYVSGEESANQIKLRADRLGIGQSNMLLYPESDMHDI
REQINDVKPDFVVIDSIQTMNEPSLDSMTGSASQVREVTSELMKIAKMDAITVFVIGHVTKEGAIAGPKIMEHMVDTVLY
FEGDEHHTYRILHSVKNRFGAANEIGMFEMVNEGLKEVTNPSAIFLDQRLPNSTGSAVVVSLEGTRPLLAEIQALVTPTA
FGYAKRTTSGIDYNRAALLLAVLEKRGNLMLQNQDVYLTATGGIKLNEPAIDLAIAMSVASSYTDKEISPTDCFVGEVGL
TGEIRRVDKIEARVKEAAKVGFKRIFIPKHNMYQGLTNNGIEVIPVSSIPQALKLVFN

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=710481 NNL98_RS01680 WP_003549080.1 326857..328233(+) (radA) [Lactobacillus acidophilus strain HN017]
ATGGCAAGGGTAAAAACTCAATATAAATGTCGCTCCTGTGGTTACATTTCTGCTAGTTATTTAGGTAGATGTCCTAACTG
TGGAGCATGGAATCAATTTGAAAAAGAAACTGAAGAAGTACAAAAACGTTCAACAAAAGCTACAGCTAGCCGTTTAATCC
AAAAAACTGGCGTAAATGAACCAGTAAAGCTAGATAAAATTAAAGCGGAAAAAGAAGAGAGAATTTCTACTAAATCAGAA
GAATTAAATCGTGTTTTAGGTGGCGGAATTGTACCTGGATCGCTTGTATTAATCGGTGGAGATCCTGGAATTGGTAAATC
AACTTTAATGTTGCAAATTATGAGTGATTTGGCAGAAAAGCATAAAGTATTATACGTTTCCGGAGAAGAATCAGCTAATC
AAATTAAGCTAAGAGCTGATCGGTTAGGAATCGGACAGAGTAACATGCTTTTATATCCTGAAAGTGATATGCATGATATT
CGTGAACAAATTAATGATGTTAAACCTGATTTTGTAGTTATTGATTCAATTCAAACAATGAATGAACCTAGCTTAGATTC
TATGACAGGTTCTGCCTCACAGGTTCGTGAAGTAACTAGTGAATTGATGAAAATCGCAAAAATGGATGCAATTACTGTTT
TTGTAATTGGACATGTAACTAAAGAGGGTGCAATTGCGGGACCTAAAATTATGGAACATATGGTAGATACTGTTCTATAT
TTTGAAGGGGATGAGCACCATACTTATAGAATTTTACATTCTGTGAAAAATCGTTTTGGTGCTGCTAATGAAATTGGCAT
GTTTGAAATGGTGAATGAAGGGTTAAAAGAAGTAACTAATCCGTCAGCTATTTTTCTTGATCAAAGATTACCAAATTCGA
CTGGTTCAGCAGTAGTTGTTTCTTTGGAAGGAACACGCCCGCTTTTGGCAGAAATTCAGGCTTTGGTTACGCCAACGGCA
TTTGGTTATGCAAAAAGAACAACGTCAGGGATTGATTATAATAGGGCTGCTCTTTTACTGGCAGTACTTGAAAAGCGTGG
TAATTTGATGTTGCAAAACCAAGATGTATATTTGACTGCTACTGGTGGGATTAAGTTGAATGAGCCTGCGATTGATTTGG
CGATTGCAATGTCTGTTGCATCTAGTTATACAGACAAAGAAATTTCACCAACTGATTGTTTTGTAGGTGAAGTAGGTTTA
ACGGGAGAAATCCGTAGAGTAGATAAAATTGAAGCTAGAGTAAAAGAAGCTGCTAAAGTTGGTTTTAAGCGAATTTTTAT
TCCTAAACACAATATGTATCAAGGATTAACTAATAACGGAATAGAAGTTATTCCAGTTTCAAGCATTCCGCAAGCGTTAA
AATTAGTTTTTAATTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-431)

AAA domain (AAA_25)

(66-223)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5FM36

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

57.549

99.782

0.574
  radA Streptococcus pneumoniae D39

57.549

99.782

0.574
  radA Streptococcus pneumoniae R6

57.549

99.782

0.574
  radA Streptococcus pneumoniae TIGR4

57.549

99.782

0.574
  radA Streptococcus mitis NCTC 12261

57.549

99.782

0.574
  radA Streptococcus mitis SK321

57.549

99.782

0.574
  radA Bacillus subtilis subsp. subtilis str. 168

56.264

99.345

0.559