Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   NLX30_RS16180 Genome accession   NZ_CP101524
Coordinates   3353805..3355310 (+) Length   501 a.a.
NCBI ID   WP_059233171.1    Uniprot ID   A0A9Q9SP11
Organism   Burkholderia arboris strain MEC_B345     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3348805..3360310
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NLX30_RS16155 (NLX30_16130) kdsD 3350049..3351032 (+) 984 WP_059233175.1 arabinose 5-phosphate isomerase KdsD -
  NLX30_RS16160 (NLX30_16135) - 3351032..3351568 (+) 537 WP_174994104.1 KdsC family phosphatase -
  NLX30_RS16165 (NLX30_16140) lptC 3351572..3352186 (+) 615 WP_059233174.1 LPS export ABC transporter periplasmic protein LptC -
  NLX30_RS16170 (NLX30_16145) lptA 3352207..3352872 (+) 666 WP_059233173.1 lipopolysaccharide transport periplasmic protein LptA -
  NLX30_RS16175 (NLX30_16150) lptB 3352869..3353645 (+) 777 WP_059233172.1 LPS export ABC transporter ATP-binding protein -
  NLX30_RS16180 (NLX30_16155) rpoN/rpoN1 3353805..3355310 (+) 1506 WP_059233171.1 RNA polymerase factor sigma-54 Machinery gene
  NLX30_RS16185 (NLX30_16160) hpf 3355468..3355827 (+) 360 WP_006477786.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  NLX30_RS16190 (NLX30_16165) - 3356023..3356535 (+) 513 WP_034195643.1 PTS sugar transporter subunit IIA -
  NLX30_RS16195 (NLX30_16170) hprK 3356653..3357621 (+) 969 WP_011353227.1 HPr(Ser) kinase/phosphatase -
  NLX30_RS16200 (NLX30_16175) rapZ 3357697..3358605 (+) 909 WP_059233170.1 RNase adapter RapZ -
  NLX30_RS16205 (NLX30_16180) - 3358633..3359268 (+) 636 WP_059233169.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54849.99 Da        Isoelectric Point: 4.9339

>NTDB_id=710387 NLX30_RS16180 WP_059233171.1 3353805..3355310(+) (rpoN/rpoN1) [Burkholderia arboris strain MEC_B345]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPGQHNEPAS
TNGSSTSGDRAERDEPAGADEYDSYGSGDGNDGPQWNLDEFGRASGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQASP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEIDCDELNAALALLHSFDPPGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVMP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=710387 NLX30_RS16180 WP_059233171.1 3353805..3355310(+) (rpoN/rpoN1) [Burkholderia arboris strain MEC_B345]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATCTGGCGCTGACGCCGCAGCTGCAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAAAACCCGTTGCTCGAGAACGACGACGAATGGA
TCGCGAGCCCGCTGCGCGTCGCGGCGGACGGATCGTTGATCGCGCAGACGCCCCCCGGGCAGCATAACGAGCCGGCCTCG
ACGAACGGCAGCAGCACGTCCGGCGATCGCGCAGAGCGCGACGAGCCGGCTGGCGCCGACGAATACGACAGTTACGGGTC
CGGCGACGGCAACGACGGCCCGCAATGGAATCTCGATGAATTCGGCCGCGCGAGCGGCGCGTCCGACGACGACGACCTCC
CGCCGCTGCAGGTGCAGGAAGCCGCGACGTCGCTGCGCGACCACCTGTCCGCGCAATTGCGCGTCACGCAGGCCAGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAGTCGCTCGACGACGACGGCTACCTGACCGCGACGCTCGACGAAGTGCT
CGCCGACCTGCCGCCCGAGCTGGAGATCGACTGCGACGAGCTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGACCCGC
CCGGCGTCGGCGCACGCTCGGCGTCGGAATGCCTGAAGCTGCAGTTGCTGCGCCTCGATCCGTCCCCGACGCGCACGCTC
GCGCTCGAGATCGTGTCGCAGCACCTCGAACTGCTCGCGGCCCGCGATTTCACGCGGTTGCGCAAGCACCTGAAGGCAAG
CGACGACGCGCTGCGCGACGCGCATGCGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCGTACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGATGGCTCGCGGAGCTCAATCCGGAGGTCATGCCG
CGCCTGCGGATCAACAACCTGTACGCAAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGTTCGCTGAAGCAGCA
GCTCCAGGAAGCACGGTGGCTGATCAAGAATATCCAGCAGCGTTTCGACACGATCCTGCGTGTCGCGCAGGCTATTGTCG
AGCGTCAGAAGAATTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTTTCGACCGACACCGGTGGCGCCGCCTCGTCGACCGCCATCCGAGCCCTCATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGTTTCGTGGTCGCACGC
CGCACGGTTGCGAAATATCGCGAAGCCCTCAAAATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.379

100

0.691