Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   E5Q65_RS10950 Genome accession   NZ_CP116480
Coordinates   2263542..2264522 (-) Length   326 a.a.
NCBI ID   WP_001055627.1    Uniprot ID   A7ZR74
Organism   Escherichia coli strain GTEN_24     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2258542..2269522
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5Q65_RS10920 yggI 2259205..2259702 (+) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -
  E5Q65_RS10925 endA 2259797..2260504 (+) 708 WP_000286500.1 deoxyribonuclease I -
  E5Q65_RS10930 rsmE 2260584..2261315 (+) 732 WP_001222508.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  E5Q65_RS10935 gshB 2261328..2262278 (+) 951 WP_000593273.1 glutathione synthase -
  E5Q65_RS10940 yqgE 2262387..2262950 (+) 564 WP_001053178.1 YqgE/AlgH family protein -
  E5Q65_RS10945 ruvX 2262950..2263366 (+) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  E5Q65_RS10950 pilT 2263542..2264522 (-) 981 WP_001055627.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  E5Q65_RS10955 yggS 2264540..2265244 (+) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  E5Q65_RS10960 yggT 2265262..2265828 (+) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  E5Q65_RS10965 yggU 2265825..2266115 (+) 291 WP_001277222.1 DUF167 family protein YggU -
  E5Q65_RS10970 rdgB 2266123..2266716 (+) 594 WP_001174745.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  E5Q65_RS10975 hemW 2266709..2267845 (+) 1137 WP_001543141.1 radical SAM family heme chaperone HemW -
  E5Q65_RS10980 - 2268162..2269148 (+) 987 WP_000784004.1 TRAP transporter substrate-binding protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35942.12 Da        Isoelectric Point: 5.7980

>NTDB_id=710277 E5Q65_RS10950 WP_001055627.1 2263542..2264522(-) (pilT) [Escherichia coli strain GTEN_24]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=710277 E5Q65_RS10950 WP_001055627.1 2263542..2264522(-) (pilT) [Escherichia coli strain GTEN_24]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGCAGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTTAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGGTTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTACGTGACAGTGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCAACCTTACATACACGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCGGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTACTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZR74

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

39.514

100

0.399
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Acinetobacter baylyi ADP1

36.957

98.773

0.365
  pilB Legionella pneumophila strain ERS1305867

30.89

100

0.362