Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoS   Type   Regulator
Locus tag   E5Q65_RS09645 Genome accession   NZ_CP116480
Coordinates   1980780..1981772 (-) Length   330 a.a.
NCBI ID   WP_000081550.1    Uniprot ID   A0A370V765
Organism   Escherichia coli strain GTEN_24     
Function   regulation of chitinases (predicted from homology)   
Competence regulation

Genomic Context

Location: 1975780..1986772
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5Q65_RS09625 otnK 1976555..1977817 (+) 1263 WP_000590392.1 3-oxo-tetronate kinase -
  E5Q65_RS09630 ygbL 1977814..1978452 (+) 639 WP_001278994.1 aldolase -
  E5Q65_RS09635 ygbM 1978457..1979233 (+) 777 WP_001136918.1 HPr family phosphocarrier protein -
  E5Q65_RS09640 ygbN 1979322..1980686 (+) 1365 WP_001402448.1 GntP family transporter -
  E5Q65_RS09645 rpoS 1980780..1981772 (-) 993 WP_000081550.1 RNA polymerase sigma factor RpoS Regulator
  E5Q65_RS09650 nlpD 1981835..1982929 (-) 1095 WP_306174120.1 murein hydrolase activator NlpD -
  E5Q65_RS09655 pcm 1983114..1983740 (-) 627 WP_000254708.1 protein-L-isoaspartate O-methyltransferase -
  E5Q65_RS09660 surE 1983734..1984495 (-) 762 WP_001295182.1 5'/3'-nucleotidase SurE -
  E5Q65_RS09665 truD 1984476..1985525 (-) 1050 WP_000568926.1 tRNA pseudouridine(13) synthase TruD -
  E5Q65_RS09670 ispF 1985522..1986001 (-) 480 WP_001219242.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  E5Q65_RS09675 ispD 1986001..1986711 (-) 711 WP_000246138.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -

Sequence

Protein


Download         Length: 330 a.a.        Molecular weight: 37972.84 Da        Isoelectric Point: 4.5597

>NTDB_id=710274 E5Q65_RS09645 WP_000081550.1 1980780..1981772(-) (rpoS) [Escherichia coli strain GTEN_24]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide


Download         Length: 993 bp        

>NTDB_id=710274 E5Q65_RS09645 WP_000081550.1 1980780..1981772(-) (rpoS) [Escherichia coli strain GTEN_24]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTTATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATTGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCATGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGTGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Domains

Predicted by InterProScan.

Sigma-70 region 3 (Sigma70_r3)

(174-248)

Sigma-70 region 2 (Sigma70_r2)

(94-163)

Sigma-70 factor, region 1.2 (Sigma70_r1_2)

(56-88)

Sigma-70, region 4 (Sigma70_r4)

(262-315)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A370V765

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoS Vibrio cholerae O1 biovar El Tor strain E7946

73.065

97.879

0.715