Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   NMG48_RS09935 Genome accession   NZ_CP101416
Coordinates   2160515..2162527 (+) Length   670 a.a.
NCBI ID   WP_271255066.1    Uniprot ID   -
Organism   Pseudanabaena sp. Chao 1811     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2155515..2167527
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMG48_RS09920 - 2156115..2156951 (+) 837 WP_271255063.1 TPM domain-containing protein -
  NMG48_RS09925 - 2156980..2157972 (-) 993 WP_271255064.1 sensor domain-containing diguanylate cyclase -
  NMG48_RS09930 - 2158061..2160121 (+) 2061 WP_271255065.1 D-alanyl-lipoteichoic acid biosynthesis protein DltD -
  NMG48_RS09935 pilB/pilB1 2160515..2162527 (+) 2013 WP_271255066.1 GspE/PulE family protein Machinery gene
  NMG48_RS09940 - 2162734..2163888 (+) 1155 WP_271255264.1 type IV pilus twitching motility protein PilT -
  NMG48_RS09945 - 2163954..2165162 (+) 1209 WP_271255067.1 type II secretion system F family protein -
  NMG48_RS09950 miaA 2165326..2166225 (-) 900 WP_271255068.1 tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA -
  NMG48_RS09955 - 2166845..2167309 (+) 465 WP_271255069.1 pentapeptide repeat-containing protein -

Sequence

Protein


Download         Length: 670 a.a.        Molecular weight: 74752.42 Da        Isoelectric Point: 5.3391

>NTDB_id=710080 NMG48_RS09935 WP_271255066.1 2160515..2162527(+) (pilB/pilB1) [Pseudanabaena sp. Chao 1811]
MNTSDQAPNTAASRNRTKALAKRGGRTPFESKLIQEGHATSDQFDRAVKDSQEQNVPFLMALEQILGQQLAPDITRYYKR
QQLFELRVLYGIEPIDPDVEVDKFDIPTLSALLDSNIVPVASCRDCEILPLIKTENSITIAVVSPDSYKVQNEIARLLKN
ITVIKRVIAREDFHRTFDNIVQFQINKSSKAEGAISDEDLQVKDDVFGEDIADTDQGDELMVGGENSAPIISLVDKILVR
ALKEGCSDIHIEPQEKDLCIRLRKDGVLREYFFLSENKRLPKNIINAVISRFKIISNLNIAEKRVPQDGKLKRNFQGRRV
DFRVNTCPTQNGEKVCLRILDNSNTQLGLNKLISDPESLELMQSFAKRPYGLILVTGPTGSGKTTTLYSTLAECNDPGIN
ISTAEDPVEYNLPGLTQCQVLREKGMTFASILRAFLRQDPDVILVGETRDAETAKTAIEAALTGHLVLTTLHTNDAPSCI
ARLEEMGVEPFMASTAIIGVLAQRLLRRVCDNCRIPYNPSQEELDRFGLPSSDLEKTFYRANIVNREAMLPGQRVCPKCN
GSGYKGRAGVYEIMKMTERLQSAINKGATTEVIKEIAVQEGMKTLMAYAFDLVRQGATTLDEVLRVVYTDKGREAEERAR
RGKGLECDNCDAMLTPETVECPYCTTPRTF

Nucleotide


Download         Length: 2013 bp        

>NTDB_id=710080 NMG48_RS09935 WP_271255066.1 2160515..2162527(+) (pilB/pilB1) [Pseudanabaena sp. Chao 1811]
ATGAATACATCAGATCAAGCGCCAAATACTGCCGCAAGCAGAAACAGAACCAAAGCTCTTGCCAAACGAGGAGGTCGTAC
TCCCTTTGAGAGCAAACTAATTCAAGAAGGACATGCTACCTCCGACCAGTTCGATCGCGCTGTCAAGGATAGTCAAGAAC
AGAATGTTCCATTTCTCATGGCCCTAGAGCAAATTTTGGGTCAACAACTTGCCCCAGACATTACCCGCTATTACAAGCGC
CAACAGCTTTTTGAACTGCGTGTCCTTTACGGCATTGAACCGATCGACCCCGATGTCGAAGTTGACAAATTTGACATCCC
TACCCTCAGTGCCTTACTTGACTCCAATATTGTCCCCGTTGCTAGTTGCCGCGACTGTGAGATCCTCCCTCTCATCAAAA
CCGAAAACAGTATTACCATTGCCGTAGTTTCACCCGACAGTTATAAAGTCCAAAACGAGATTGCGCGACTGCTCAAAAAT
ATAACGGTGATTAAGCGAGTGATTGCTCGTGAGGATTTTCATCGCACATTTGATAATATTGTGCAGTTTCAAATCAACAA
GAGTTCTAAAGCTGAAGGTGCAATCAGTGACGAAGATCTTCAAGTTAAAGATGATGTCTTTGGTGAAGATATAGCTGATA
CGGATCAAGGTGATGAACTTATGGTTGGTGGCGAAAACTCCGCCCCAATCATTAGCCTTGTAGATAAAATCTTAGTCAGA
GCGCTCAAAGAAGGTTGCTCAGATATTCACATCGAGCCACAGGAAAAAGATCTTTGCATTCGTCTGCGTAAGGATGGTGT
ATTGCGCGAGTACTTCTTCCTCAGCGAAAACAAGCGTTTACCCAAGAACATTATCAACGCTGTCATCTCTCGCTTCAAGA
TTATTTCTAACCTCAATATCGCTGAGAAAAGGGTTCCCCAAGATGGCAAGCTGAAGCGTAACTTCCAAGGTCGTCGCGTA
GATTTCCGCGTAAATACCTGCCCCACTCAAAATGGTGAAAAAGTATGTCTACGGATTCTCGATAACTCCAATACCCAACT
CGGACTGAATAAACTGATCAGTGATCCCGAAAGCTTGGAACTGATGCAAAGTTTTGCCAAACGTCCCTATGGACTGATTC
TAGTTACAGGTCCTACGGGGTCAGGTAAAACCACCACTCTATACTCAACCCTCGCTGAATGTAACGATCCCGGTATTAAC
ATTAGTACCGCCGAAGATCCTGTCGAATATAATCTCCCTGGTCTGACCCAATGCCAAGTCCTACGGGAAAAAGGCATGAC
TTTCGCCAGTATTCTCCGCGCCTTCCTCCGTCAAGATCCCGACGTAATCCTTGTGGGTGAGACCCGTGATGCAGAAACCG
CTAAAACTGCGATCGAAGCGGCGCTGACAGGTCACTTAGTCTTAACCACGCTACATACCAACGATGCTCCTAGTTGTATT
GCCCGTCTTGAAGAAATGGGTGTCGAACCATTCATGGCAAGTACAGCGATTATTGGTGTACTTGCTCAGCGTCTACTCCG
CCGAGTCTGTGACAACTGTCGCATCCCCTACAATCCATCGCAGGAAGAACTTGATCGCTTTGGCTTGCCCAGTAGCGATC
TTGAAAAGACCTTCTACCGAGCTAATATCGTTAACCGTGAAGCTATGCTCCCTGGGCAGCGCGTATGTCCAAAATGTAAC
GGCTCTGGATACAAGGGTCGGGCAGGGGTTTACGAAATCATGAAAATGACCGAGCGTCTGCAAAGTGCGATTAACAAGGG
GGCAACCACCGAAGTGATCAAAGAAATTGCCGTCCAAGAAGGGATGAAAACCTTAATGGCATATGCCTTTGACTTAGTCA
GGCAGGGTGCAACTACTCTTGATGAAGTGCTGCGTGTAGTTTACACAGACAAAGGAAGAGAAGCCGAAGAACGTGCGCGT
CGAGGCAAAGGACTTGAATGCGACAACTGTGATGCTATGCTCACACCTGAAACCGTCGAATGTCCTTACTGCACCACACC
GAGAACCTTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(71-180)

Type II/IV secretion system protein (T2SSE)

(236-508)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

51.642

100

0.516