Detailed information    

insolico Bioinformatically predicted

Overview

Name   helD   Type   Machinery gene
Locus tag   PIB33_RS18335 Genome accession   NZ_CP116391
Coordinates   3449398..3451722 (-) Length   774 a.a.
NCBI ID   WP_032677046.1    Uniprot ID   -
Organism   Bacillus subtilis strain GXD-20     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3444398..3456722
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PIB33_RS18325 (PIB33_18325) cysI 3445669..3447384 (-) 1716 WP_021480597.1 assimilatory sulfite reductase (NADPH) hemoprotein subunit -
  PIB33_RS18330 (PIB33_18330) cysJ 3447410..3449227 (-) 1818 WP_032722511.1 assimilatory sulfite reductase (NADPH) flavoprotein subunit -
  PIB33_RS18335 (PIB33_18335) helD 3449398..3451722 (-) 2325 WP_032677046.1 DNA helicase IV Machinery gene
  PIB33_RS18340 (PIB33_18340) - 3452300..3452734 (+) 435 WP_014477979.1 hypothetical protein -
  PIB33_RS18345 (PIB33_18345) yvgT 3452844..3453455 (-) 612 WP_032722513.1 trimeric intracellular cation channel family protein -
  PIB33_RS18350 (PIB33_18350) bdbC 3453641..3454057 (-) 417 WP_003228417.1 disulfide oxidoreductase -
  PIB33_RS18355 (PIB33_18355) bdbD 3454062..3454730 (-) 669 WP_003228414.1 disulfide bond formation protein BdbD -

Sequence

Protein


Download         Length: 774 a.a.        Molecular weight: 89894.54 Da        Isoelectric Point: 6.5133

>NTDB_id=709785 PIB33_RS18335 WP_032677046.1 3449398..3451722(-) (helD) [Bacillus subtilis strain GXD-20]
MNQQDKEWKEEQSRIDEVLQELEKKERFLETSAGGLKHDIIGLRKSFWEDVKVNFDDAHEAIETMASIKQQAELLSDREQ
NHRRMDQQLKRIHRLKKSPYFGRIDFIENGEAEAERIYIGLASCLDEKEEHFLIYDWRAPISSLYYNYSPGKAEYEVPGE
TIEGEMVLKRQFMIKNGDLKAMFNTDMTIGDEMLQEVLSHHSDTQMKNIVSTIQKEQNQIIRNEKSKVLIVQGAAGSGKT
SAALQRVAYLLYRHRGVIDAGQIVLFSPNFLFNSYVSSVLPELGEENMEQATFQEYIEHRLGRKFKCESPFDQLEYCLTE
TKGGDFPTRLAGITWKAGLSFQQFINEYVTRLSSEGMIFKNIIFRGQKLITKEQIQSYFYSLDQNHSIPNRMEQTAKWLL
SELNKLEKKERRKDWVVHEAELLDKEDYLDVYKKLQERKRFSESTFNDYQREQQLLAAIIVKKAFKPLKQAVRLLAFLDV
SQLYLQLFSGWGGKFQHEKMAVIGELTRSAFTDNKLLYEDAAPFLYMQDLIEGRKKNTKIKHLFIDEAQDYSPFQMAYMR
SIFPAASMTVLGDINQSIYAHTIHGDQRMDACFEDDPAEYVRLKRTYRSTRQIVEFTKAMLQDGADIEPFNRSGEMPLVV
KTEGHESLCQKLAQEIGRLKKKGRETIAVICKTAQQCIQAHAHMSEYTDVRLIHKENQPFQKGVCVIPVYLAKGIEFDAV
LVYDASEEHYHTEHDRRLLYTACTRAMHMLAVFYTGEASPFVTAVPPHLYQMAE

Nucleotide


Download         Length: 2325 bp        

>NTDB_id=709785 PIB33_RS18335 WP_032677046.1 3449398..3451722(-) (helD) [Bacillus subtilis strain GXD-20]
ATGAATCAGCAGGATAAGGAATGGAAGGAAGAGCAGTCGAGAATAGATGAGGTGCTGCAGGAGCTCGAAAAAAAAGAGCG
GTTTCTGGAAACGTCTGCGGGCGGGTTGAAGCATGATATCATCGGATTAAGAAAAAGCTTTTGGGAGGATGTTAAGGTTA
ATTTTGATGATGCCCATGAAGCGATTGAAACGATGGCAAGCATTAAGCAGCAGGCAGAGCTGCTGTCTGATCGGGAACAG
AATCATCGGCGCATGGATCAGCAGCTCAAACGAATTCATCGATTGAAAAAATCGCCTTATTTCGGCCGGATTGATTTTAT
TGAAAACGGCGAGGCGGAGGCAGAGCGAATTTATATCGGTTTAGCTTCCTGTTTGGATGAGAAGGAGGAACACTTTCTAA
TCTATGACTGGAGGGCGCCGATATCAAGTTTGTACTACAATTATTCCCCAGGAAAAGCGGAGTACGAGGTGCCCGGTGAA
ACGATAGAAGGGGAAATGGTGCTGAAACGCCAGTTTATGATCAAAAACGGCGATCTCAAGGCGATGTTTAATACCGATAT
GACGATTGGTGATGAAATGCTGCAAGAGGTATTAAGCCATCACTCCGATACCCAAATGAAAAACATCGTGTCTACGATTC
AGAAAGAACAAAATCAGATTATCCGTAATGAAAAAAGCAAGGTCCTGATCGTGCAGGGAGCGGCTGGCAGCGGGAAAACA
TCGGCTGCGCTTCAGCGTGTCGCTTATTTGTTATACAGGCACCGCGGTGTCATTGATGCCGGACAAATCGTTTTGTTTTC
TCCTAACTTCTTATTTAACAGCTATGTCTCCTCTGTTTTGCCGGAGTTAGGAGAAGAGAATATGGAGCAGGCGACGTTTC
AGGAATATATCGAGCATCGTCTTGGCCGCAAGTTCAAATGTGAAAGCCCCTTTGATCAGCTCGAATATTGCCTGACCGAA
ACAAAGGGAGGCGATTTTCCAACAAGGCTTGCGGGAATCACATGGAAGGCGGGTTTATCCTTTCAGCAATTCATTAATGA
ATACGTCACACGGCTTTCCTCTGAAGGGATGATTTTTAAAAATATTATATTCCGCGGACAAAAGCTGATCACAAAGGAGC
AAATTCAATCCTATTTTTACTCATTGGATCAAAATCACTCCATTCCGAATCGGATGGAGCAAACCGCGAAGTGGCTGCTG
AGTGAGCTTAACAAGCTTGAGAAAAAGGAACGGCGAAAGGATTGGGTTGTTCATGAAGCTGAGCTGCTTGACAAAGAGGA
CTACCTGGACGTGTACAAAAAACTGCAGGAAAGGAAACGGTTCAGCGAGAGTACGTTTAACGATTATCAAAGAGAGCAGC
AGCTGCTTGCCGCCATCATTGTGAAAAAAGCTTTCAAACCGCTCAAGCAGGCAGTCAGGCTCCTTGCATTTCTTGATGTT
TCACAGCTGTATTTGCAATTGTTTTCTGGCTGGGGAGGGAAATTTCAGCATGAGAAGATGGCCGTGATAGGTGAGCTGAC
CCGTTCGGCATTTACGGATAATAAATTGCTCTATGAGGACGCGGCACCGTTTCTTTATATGCAGGATCTGATTGAGGGCA
GAAAGAAAAATACGAAGATCAAACATCTATTTATTGACGAAGCCCAGGACTATTCACCGTTTCAGATGGCTTACATGAGA
AGTATTTTTCCGGCTGCCAGCATGACAGTGCTCGGTGATATCAATCAGTCCATCTACGCGCATACCATACACGGGGATCA
GCGTATGGATGCGTGCTTTGAGGATGATCCTGCTGAGTATGTTCGTTTAAAGAGAACATACAGATCAACAAGGCAGATTG
TCGAGTTTACCAAAGCGATGCTTCAAGATGGAGCGGATATTGAGCCGTTTAATAGAAGCGGGGAAATGCCGCTCGTTGTC
AAAACTGAAGGACATGAGAGCCTTTGCCAAAAGCTCGCGCAAGAAATTGGCCGGCTCAAGAAAAAAGGGCGCGAAACAAT
AGCCGTCATCTGCAAGACTGCGCAGCAATGCATACAGGCGCACGCCCACATGAGTGAATATACAGATGTTCGCCTGATTC
ATAAAGAAAATCAACCATTTCAAAAGGGTGTTTGCGTGATTCCGGTGTATTTAGCGAAGGGAATTGAATTTGACGCAGTG
CTTGTCTATGATGCATCTGAAGAACACTATCATACAGAGCACGACCGCAGGCTTTTATATACTGCGTGCACCCGTGCGAT
GCACATGCTGGCAGTATTTTATACAGGTGAAGCCAGTCCGTTTGTAACCGCTGTGCCGCCTCACTTATATCAGATGGCTG
AATGA

Domains

Predicted by InterProScan.

UvrD-like helicase C-terminal domain (UvrD_C_2)

(704-751)

AAA domain (AAA_19)

(217-580)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  helD Bacillus subtilis subsp. subtilis str. 168

98.062

100

0.981