Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   NMT99_RS12445 Genome accession   NZ_CP101290
Coordinates   2419879..2420949 (-) Length   356 a.a.
NCBI ID   WP_004399124.1    Uniprot ID   A0A809FNW6
Organism   Bacillus subtilis strain LjM2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2414879..2425949
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMT99_RS12400 tapA 2415358..2416119 (-) 762 WP_029726724.1 amyloid fiber anchoring/assembly protein TapA -
  NMT99_RS12405 yqzG 2416390..2416716 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  NMT99_RS12410 spoIITA 2416758..2416937 (-) 180 WP_029726723.1 YqzE family protein -
  NMT99_RS12415 comGG 2417009..2417383 (-) 375 WP_029726722.1 ComG operon protein ComGG Machinery gene
  NMT99_RS12420 comGF 2417384..2417767 (-) 384 WP_029726721.1 ComG operon protein ComGF Machinery gene
  NMT99_RS12425 comGE 2417793..2418140 (-) 348 WP_014480255.1 ComG operon protein 5 Machinery gene
  NMT99_RS12430 comGD 2418124..2418555 (-) 432 WP_029726720.1 comG operon protein ComGD Machinery gene
  NMT99_RS12435 comGC 2418545..2418841 (-) 297 WP_029726719.1 comG operon protein ComGC Machinery gene
  NMT99_RS12440 comGB 2418855..2419892 (-) 1038 WP_029726718.1 comG operon protein ComGB Machinery gene
  NMT99_RS12445 comGA 2419879..2420949 (-) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  NMT99_RS12450 - 2421162..2421359 (-) 198 WP_029726717.1 hypothetical protein -
  NMT99_RS12455 corA 2421361..2422314 (-) 954 WP_004399136.1 magnesium transporter CorA -
  NMT99_RS12460 yqhB 2422457..2423785 (+) 1329 WP_029726716.1 hemolysin family protein -
  NMT99_RS12465 rsbRD 2423838..2424674 (-) 837 WP_003230149.1 RsbT co-antagonist protein RsbRD -
  NMT99_RS12475 mgsR 2424898..2425278 (-) 381 WP_003230147.1 transcriptional regulator MgsR -
  NMT99_RS12480 yqgY 2425510..2425755 (+) 246 WP_003226300.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40458.64 Da        Isoelectric Point: 9.0175

>NTDB_id=709484 NMT99_RS12445 WP_004399124.1 2419879..2420949(-) (comGA) [Bacillus subtilis strain LjM2]
MDSIEKVSKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRDMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=709484 NMT99_RS12445 WP_004399124.1 2419879..2420949(-) (comGA) [Bacillus subtilis strain LjM2]
TTGGATTCAATAGAAAAGGTAAGCAAAAACTTGATTGAAGAGGCATATCTAACAAAGGCTTCTGATATTCACATTGTGCC
GAGGGAGCGGGACGCTATCATTCATTTTCGGGTCGATCATGCCTTGCTGAAAAAAAGGGACATGAAAAAAGAAGAGTGCG
TAAGGCTGATTTCACATTTTAAATTTCTTTCAGCAATGGATATAGGTGAAAGGCGAAAGCCGCAAAACGGTTCGCTTACG
TTAAAGCTGAAAGAGGGAAATGTTCATTTAAGAATGTCAACGCTGCCCACAATTAATGAAGAAAGCCTCGTGATCAGAGT
GATGCCCCAATACAATATCCCTTCGATTGATAAATTGTCGCTATTTCCGAAGACAGGAGCCACATTACTCTCGTTTTTAA
AACATTCCCATGGCATGCTCATTTTTACCGGGCCAACTGGTTCAGGGAAGACTACCACACTATACTCTCTCGTTCAATAT
GCAAAAAAACACTTTAATCGAAATATTGTCACATTAGAGGACCCTGTTGAAACAAGGGACGAAGATGTTCTTCAGGTTCA
GGTGAATGAAAAAGCCGGTGTAACTTATTCCGCAGGTCTGAAAGCAATTTTGCGCCATGACCCCGATATGATTATTTTAG
GTGAGATCAGAGACGCGGAAACAGCTGAAATTGCGGTGCGGGCAGCGATGACGGGACATCTGGTACTAACGAGCCTTCAT
ACGAGAGACGCAAAGGGCGCAATTTACAGACTGCTTGAATTCGGTATCAATATGAATGAAATCGAACAGACTGTCATTGC
AATAGCGGCTCAGCGCTTGGTTGATTTGGCTTGCCCGTTTTGTGAAAACGGATGTTCATCAGTGTATTGCCGACAGTCAC
GAAATACCAGGAGAGCTAGCGTTTATGAGCTTCTATACGGGAAAAATCTTCAGCAATGTATCCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACGCTTCGTCAAATTATCAGAAAAGGAATTGCGCTCGGCTATTTAACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A809FNW6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

100

100

1