Detailed information    

experimental Experimentally validated

Overview

Name   pilC   Type   Machinery gene
Locus tag   - Genome accession   Z54202
Coordinates   1..3114 (+) Length   1037 a.a.
NCBI ID   CAA90909.1    Uniprot ID   -
Organism   Neisseria meningitidis A1493     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

PilC functions in meningococcal class II pilus assembly and adherence. Expression of the cloned N. meningitidis pilC gene in a gonococcal pilC1,2 mutant restores pilus assembly, adherence to ME-180 epithelial cells, and transformation competence to the wild-type level.


Genomic Context

Location: 1..8114
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Locus_0 pilC 1..3114 (+) 3114 CAA90909.1 PilC Machinery gene

Sequence

Protein


Download         Length: 1037 a.a.        Molecular weight: 113701.41 Da        Isoelectric Point: 9.4904

>NTDB_id=1090 CAA90909.1 1..3114(+) (pilC) [Neisseria meningitidis A1493]
MNKTLKRQVFRHTALYAAILMFSHTGGGGAMAQTHKYAIIMNERNQLEVKGNGQYSTIKDKDRERKFIYNKDRGGGGSVF
FDNTDTLVSQQRGTAVFGTATYLPPYGKVSGFDADGLQKRNNAVDWIHTTQAGLAGYAYTDVICRSNQCPQLVYETKFSF
DGIGLAKNAGSLDRHPDPSRENSPIYKLKDHPWLGVSFNLGSENTVKDGKSFNKLISSFSEGNNNQTIVSTTRGHSISLS
DWKREHTAMAYYLNAKLHLLDKKGIEDIAQGKTVDLGTLRPRVEATVRRGELLNFWATWKIEDKGNITVRLGLPEVKAGR
CVNKANPNPNAKAPSPALTAPALWFGPVKDGKAEMYSASVSTYPDSSSSRIYLQNLKRKTDPGKPGRHSLETLTENDIKS
REPNFTGRQTIIRLNGGVREIKLDRNNTEVVNFNGNDGNNDTFGIVKDLGVEPDTSEWKKVLLPWTVRGFADDNKFKAFN
KEENNDNKPKYSQKYRSRDNNKGERNLGDIVNSPIVAVGEYLATSANDGMVHIFKQSGGDKRSYNLKLSYIPGTMPRKDI
QNTESTLAKDVRTFAEKGYVGDRYGVDGGFVLRKVDNLNGQNRVFMFGAMGFGGRGAYALDLTKADGSDPTAVSLFDVKD
NGNNGNNRVELGYTVGTPQIGKTHDGKYAAFLASGYATKEIITSGDNKTALYVYDLEGNGTNNLIKKIEVPGGKGGLSSP
TLVDKDLDGTVDIAYAGDRGGNMYRFDLSSQDPQQWSVRTIFEGTKPITSAPAISQLKDKRVVIFGTGSDLSEEDVDNME
EQYIYGIFDDDTATTGTVNFSDSGGGLLEQVLRRDNDNKTLFLTDYKRSDGSGNKGWVVKLKDGQRVTVKPTVVLRTAFV
TIHKYTGTDKCGAETAILGINTADGGKLTKKSARPIVPEANTAVAQYSGHKKGTNGKSIPIGCMQKSNEIVCPNGYVYDK
PVNVRYLDEKKTDGFSTTADGDAGGSGIDPAGKRSGKNNRCFSQKGVRTLLMNDLDSLDITGPTCGMKRISWREVFY*

Nucleotide


Download         Length: 3114 bp        

>NTDB_id=1090 CAA90909.1 1..3114(+) (pilC) [Neisseria meningitidis A1493]
ATGAATAAAACTTTAAAAAGGCAGGTTTTCCGCCATACCGCGCTTTATGCCGCCATCTTGATGTTTTCCCATACCGGCGG
GGGGGGGGCGATGGCGCAAACCCATAAATACGCTATTATCATGAACGAGCGAAACCAGCTCGAGGTAAAGGGGAATGGGC
AATATTCAACAATAAAGGACAAAGACAGGGAACGCAAATTTATCTATAATAAAGACAGAGGGGGTGGAGGCTCTGTCTTT
TTCGACAATACCGATACCCTTGTTTCCCAACAAAGAGGTACTGCCGTTTTTGGCACAGCCACCTACCTGCCGCCCTACGG
CAAGGTTTCCGGTTTTGATGCCGACGGGCTGCAAAAGCGCAACAATGCCGTCGATTGGATTCATACCACCCAGGCCGGGC
TGGCAGGCTACGCCTACACCGACGTCATATGCAGAAGCAACCAATGCCCCCAACTTGTCTATGAGACCAAATTTTCCTTC
GACGGCATCGGTTTGGCAAAAAATGCGGGCAGCCTGGATAGGCACCCGGACCCAAGCCGCGAAAATTCGCCCATTTACAA
ATTGAAAGATCATCCATGGTTGGGCGTATCTTTCAATTTGGGCAGCGAGAATACCGTCAAAGATGGCAAATCATTCAACA
AATTGATATCTTCTTTTAGTGAAGGCAATAATAATCAAACCATCGTCTCTACGACACGAGGCCACTCTATTTCCCTTAGC
GACTGGAAGCGCGAACATACCGCCATGGCCTATTATCTGAACGCCAAACTGCACCTGCTGGACAAAAAAGGGATTGAAGA
TATCGCCCAAGGCAAAACAGTGGATTTGGGCACCTTGAGACCGCGCGTCGAGGCAACGGTAAGGCGGGGGGAGCTGCTAA
ATTTTTGGGCTACGTGGAAGATTGAAGATAAAGGGAACATTACAGTCCGCCTCGGCCTGCCGGAAGTCAAAGCAGGCCGC
TGCGTCAACAAAGCGAACCCCAATCCCAACGCCAAAGCCCCCTCCCCCGCACTGACCGCCCCCGCGCTGTGGTTCGGACC
TGTGAAAGATGGTAAGGCGGAGATGTATTCCGCTTCGGTTTCTACCTACCCCGACAGTTCGAGCAGCCGCATCTACCTTC
AAAATCTGAAAAGAAAAACCGACCCCGGCAAACCCGGCCGCCATTCCCTCGAAACCTTGACTGAGAATGATATTAAAAGT
CGAGAGCCGAATTTCACAGGGCGGCAAACCATCATCCGATTGAATGGCGGCGTACGTGAGATCAAACTGGATAGAAACAA
TACTGAGGTCGTCAATTTTAATGGAAATGACGGCAACAACGACACTTTCGGCATTGTTAAGGACTTGGGCGTCGAACCTG
ATACCAGCGAGTGGAAAAAAGTATTGCTGCCTTGGACGGTTCGGGGTTTTGCTGATGACAATAAATTTAAAGCATTCAAC
AAAGAAGAAAACAACGACAACAAGCCAAAATACAGCCAAAAATACCGCAGCCGCGACAACAACAAGGGCGAACGCAATTT
GGGCGACATCGTCAACAGCCCCATCGTGGCGGTCGGCGAGTATTTGGCTACTTCCGCCAACGACGGGATGGTGCATATCT
TCAAACAAAGCGGCGGGGACAAGCGCAGCTACAATCTGAAGCTCAGCTACATCCCTGGAACGATGCCGCGCAAGGATATT
CAAAACACCGAATCCACCCTTGCCAAAGACGTGCGCACCTTTGCCGAAAAAGGCTATGTGGGCGACCGCTACGGCGTGGA
CGGCGGCTTTGTCTTGCGCAAAGTTGATAACTTAAACGGGCAAAACCGCGTGTTTATGTTCGGCGCGATGGGCTTTGGCG
GCAGAGGCGCGTATGCCTTGGATTTGACCAAAGCCGACGGCAGTGACCCGACCGCCGTTTCCCTGTTTGATGTAAAAGAT
AACGGCAATAATGGCAATAATCGCGTGGAATTAGGCTACACCGTCGGCACGCCGCAAATCGGCAAAACCCACGACGGCAA
ATACGCCGCCTTCCTCGCCTCCGGTTATGCGACTAAAGAAATTATTACCAGCGGCGACAATAAAACCGCGCTGTATGTGT
ATGATTTGGAAGGAAACGGTACGAATAATCTGATTAAAAAAATCGAAGTACCCGGCGGCAAGGGCGGGCTTTCGTCCCCC
ACGCTGGTGGATAAAGATTTGGACGGCACGGTCGATATCGCCTATGCCGGCGATCGCGGCGGGAATATGTACCGCTTTGA
TTTGAGCAGTCAAGATCCTCAACAATGGTCTGTACGCACTATTTTTGAAGGCACAAAACCGATTACTTCCGCGCCCGCTA
TTTCCCAACTGAAAGACAAACGCGTGGTCATCTTCGGCACGGGCAGCGATTTGAGTGAAGAGGATGTGGACAATATGGAA
GAACAATATATTTACGGTATCTTCGACGACGATACGGCGACGACGGGTACTGTAAACTTCAGCGATTCGGGAGGCGGGCT
GCTTGAGCAAGTGCTTCGTAGGGATAACGACAATAAAACCTTATTCCTGACCGATTACAAGCGATCCGACGGATCGGGCA
ATAAGGGCTGGGTAGTGAAATTGAAGGACGGACAGCGCGTTACCGTCAAACCGACCGTGGTATTGCGTACCGCCTTTGTA
ACCATCCATAAATATACGGGTACGGACAAATGCGGCGCGGAAACCGCCATTTTGGGCATCAATACCGCCGACGGCGGCAA
GCTGACCAAGAAAAGCGCGCGCCCGATTGTGCCGGAAGCCAATACGGCTGTCGCGCAATATTCCGGCCATAAGAAAGGCA
CCAACGGCAAATCCATCCCTATAGGTTGTATGCAAAAAAGCAATGAAATCGTCTGCCCGAACGGATATGTTTACGACAAA
CCGGTTAATGTGCGTTATCTGGATGAAAAGAAAACAGACGGATTTTCAACAACGGCAGACGGCGATGCGGGCGGCAGCGG
TATAGACCCCGCCGGCAAGCGTTCCGGCAAAAACAACCGCTGCTTCTCCCAAAAAGGGGTGCGCACCCTGCTGATGAACG
ATTTGGACAGCTTGGACATTACCGGCCCGACGTGCGGTATGAAACGAATCAGCTGGCGTGAAGTCTTCTACTGA

Domains

Predicted by InterproScan.

Neisseria PilC beta-propeller domain (T4P_PilY1)

(507-844)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] R R Ryll et al. (1997) PilC of Neisseria meningitidis is involved in class II pilus formation and restores pilus assembly, natural transformation competence and adherence to epithelial cells in PilC-deficient gonococci. Molecular Microbiology 23(5):879-92. [PMID: 9076726]