Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   NMT99_RS12440 Genome accession   NZ_CP101290
Coordinates   2418855..2419892 (-) Length   345 a.a.
NCBI ID   WP_029726718.1    Uniprot ID   -
Organism   Bacillus subtilis strain LjM2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2413855..2424892
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMT99_RS12390 tasA 2413953..2414738 (-) 786 WP_014664586.1 biofilm matrix protein TasA -
  NMT99_RS12395 sipW 2414802..2415374 (-) 573 WP_072692741.1 signal peptidase I SipW -
  NMT99_RS12400 tapA 2415358..2416119 (-) 762 WP_029726724.1 amyloid fiber anchoring/assembly protein TapA -
  NMT99_RS12405 yqzG 2416390..2416716 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  NMT99_RS12410 spoIITA 2416758..2416937 (-) 180 WP_029726723.1 YqzE family protein -
  NMT99_RS12415 comGG 2417009..2417383 (-) 375 WP_029726722.1 ComG operon protein ComGG Machinery gene
  NMT99_RS12420 comGF 2417384..2417767 (-) 384 WP_029726721.1 ComG operon protein ComGF Machinery gene
  NMT99_RS12425 comGE 2417793..2418140 (-) 348 WP_014480255.1 ComG operon protein 5 Machinery gene
  NMT99_RS12430 comGD 2418124..2418555 (-) 432 WP_029726720.1 comG operon protein ComGD Machinery gene
  NMT99_RS12435 comGC 2418545..2418841 (-) 297 WP_029726719.1 comG operon protein ComGC Machinery gene
  NMT99_RS12440 comGB 2418855..2419892 (-) 1038 WP_029726718.1 comG operon protein ComGB Machinery gene
  NMT99_RS12445 comGA 2419879..2420949 (-) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  NMT99_RS12450 - 2421162..2421359 (-) 198 WP_029726717.1 hypothetical protein -
  NMT99_RS12455 corA 2421361..2422314 (-) 954 WP_004399136.1 magnesium transporter CorA -
  NMT99_RS12460 yqhB 2422457..2423785 (+) 1329 WP_029726716.1 hemolysin family protein -
  NMT99_RS12465 rsbRD 2423838..2424674 (-) 837 WP_003230149.1 RsbT co-antagonist protein RsbRD -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 39702.10 Da        Isoelectric Point: 9.6257

>NTDB_id=709483 NMT99_RS12440 WP_029726718.1 2418855..2419892(-) (comGB) [Bacillus subtilis strain LjM2]
MKKIRKVWSLKDQARLLKRLGEMTAGGYTLLDGLRLMELQMNKRQAADLTDSVTCLREGAPFYQVLKSLSFHKEAVGICY
FAETHGELPASMIQSGELLERKIAQADQLKRVLRYPLFLIFTVAVMFYMLQSIIIPQFSGIYQSMNMETSRSTDILFAFF
QHIDLVIILLVLFTAGIGIYYWLVFKKKSPARQMLICIRIPLVGKLVKLFNSYFFSLQLSSLLKSGLSIYDSLNAFKHQT
FLPFYRCEAEQLIERLKAGESIESAICGSPFYETDLSKVISHGQLSGRLDRELFTYSQFILQRLEHKAQKWTGILQPMIY
GFVAAMILLVYLSMLVPMYQMMNQM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=709483 NMT99_RS12440 WP_029726718.1 2418855..2419892(-) (comGB) [Bacillus subtilis strain LjM2]
ATGAAAAAGATTAGAAAAGTCTGGTCGTTAAAGGATCAAGCGAGGTTATTAAAGAGGCTCGGTGAAATGACTGCGGGCGG
ATATACGCTTCTGGATGGATTACGCCTGATGGAACTTCAGATGAATAAGAGGCAGGCGGCTGACTTGACTGATTCGGTCA
CTTGTTTGAGGGAAGGGGCTCCGTTTTATCAAGTACTAAAGAGTTTGTCATTTCATAAGGAAGCCGTAGGTATTTGTTAT
TTTGCTGAAACACATGGTGAACTGCCTGCTTCCATGATCCAGAGCGGAGAGCTGCTGGAACGAAAAATTGCACAGGCAGA
CCAGCTGAAAAGAGTGCTGCGCTATCCGCTTTTCCTCATCTTTACGGTCGCTGTCATGTTTTATATGTTACAGTCCATCA
TCATTCCTCAGTTTTCCGGTATCTATCAATCGATGAATATGGAAACCTCACGTTCAACCGATATACTTTTTGCTTTTTTT
CAGCATATTGATCTTGTGATCATTTTGCTTGTTCTTTTTACAGCAGGTATCGGGATTTATTATTGGCTTGTGTTTAAGAA
AAAATCACCTGCCCGGCAAATGCTGATTTGTATCAGGATTCCTTTGGTTGGAAAGCTTGTCAAGCTGTTTAACAGCTACT
TTTTTTCTTTGCAGCTAAGCAGCCTTTTAAAATCAGGCCTCTCAATTTATGACAGCCTTAATGCATTTAAACATCAAACG
TTTCTCCCTTTCTACCGCTGCGAGGCTGAACAATTGATTGAACGGCTAAAAGCCGGTGAGTCAATTGAATCCGCTATTTG
TGGAAGCCCTTTTTATGAAACTGATTTATCAAAAGTCATATCTCACGGCCAGCTGAGCGGCCGATTGGATCGGGAGCTTT
TCACATACAGCCAATTCATATTACAGCGGCTGGAACACAAAGCGCAAAAATGGACAGGCATCCTTCAGCCAATGATTTAT
GGATTTGTTGCAGCGATGATCTTACTTGTGTATTTATCTATGCTTGTGCCTATGTATCAGATGATGAATCAAATGTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-337)

Type II secretion system (T2SS), protein F (T2SSF)

(17-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

99.381

93.623

0.93