Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   NMT99_RS01970 Genome accession   NZ_CP101290
Coordinates   370384..370833 (-) Length   149 a.a.
NCBI ID   WP_003234608.1    Uniprot ID   A0A8E0S750
Organism   Bacillus subtilis strain LjM2     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 365384..375833
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMT99_RS01935 yckA 365535..366215 (-) 681 WP_003234624.1 amino acid ABC transporter permease -
  NMT99_RS01940 yckB 366225..367088 (-) 864 WP_009966510.1 transporter substrate-binding domain-containing protein -
  NMT99_RS01945 - 367250..367447 (+) 198 WP_119122841.1 hypothetical protein -
  NMT99_RS01950 yckC 367466..367921 (+) 456 WP_003246356.1 RDD family protein -
  NMT99_RS01955 yckD 368003..368335 (+) 333 WP_003246247.1 YckD family protein -
  NMT99_RS01960 bglC 368489..369922 (+) 1434 WP_003246242.1 6-phospho-beta-glucosidase -
  NMT99_RS01965 nin/comJ 369959..370357 (-) 399 WP_015715226.1 DNA-entry nuclease inhibitor Regulator
  NMT99_RS01970 nucA/comI 370384..370833 (-) 450 WP_003234608.1 DNA-entry nuclease Machinery gene
  NMT99_RS01975 tlpC 371001..372722 (-) 1722 WP_029727077.1 methyl-accepting chemotaxis protein TlpC -
  NMT99_RS01980 hxlB 372833..373390 (-) 558 WP_003234605.1 6-phospho-3-hexuloisomerase -
  NMT99_RS01985 hxlA 373396..374028 (-) 633 WP_003234604.1 3-hexulose-6-phosphate synthase -
  NMT99_RS01990 hxlR 374261..374623 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16470.42 Da        Isoelectric Point: 4.5282

>NTDB_id=709443 NMT99_RS01970 WP_003234608.1 370384..370833(-) (nucA/comI) [Bacillus subtilis strain LjM2]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSTDQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=709443 NMT99_RS01970 WP_003234608.1 370384..370833(-) (nucA/comI) [Bacillus subtilis strain LjM2]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTTTCAACTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGACACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

97.987

100

0.98