Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   PIB63_RS20270 Genome accession   NZ_CP116194
Coordinates   4149343..4150776 (+) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli strain DETEC-E1005     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4144343..4155776
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PIB63_RS20240 (PIB63_20240) yrbG 4144901..4145878 (+) 978 WP_000922880.1 calcium/sodium antiporter -
  PIB63_RS20245 (PIB63_20245) kdsD 4145892..4146878 (+) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  PIB63_RS20250 (PIB63_20250) kdsC 4146899..4147465 (+) 567 WP_000030015.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  PIB63_RS20255 (PIB63_20255) lptC 4147462..4148037 (+) 576 WP_001539833.1 LPS export ABC transporter periplasmic protein LptC -
  PIB63_RS20260 (PIB63_20260) lptA 4148006..4148563 (+) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  PIB63_RS20265 (PIB63_20265) lptB 4148570..4149295 (+) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  PIB63_RS20270 (PIB63_20270) rpoN/rpoN1 4149343..4150776 (+) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  PIB63_RS20275 (PIB63_20275) hpf 4150799..4151086 (+) 288 WP_001176599.1 ribosome hibernation promoting factor -
  PIB63_RS20280 (PIB63_20280) ptsN 4151204..4151695 (+) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  PIB63_RS20285 (PIB63_20285) rapZ 4151741..4152595 (+) 855 WP_000243741.1 RNase adapter RapZ -
  PIB63_RS20290 (PIB63_20290) npr 4152592..4152864 (+) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  PIB63_RS20295 (PIB63_20295) yrbL 4153078..4153710 (+) 633 WP_001539838.1 PhoP regulatory network protein YrbL -
  PIB63_RS20300 (PIB63_20300) mtgA 4153707..4154435 (-) 729 WP_000047069.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  PIB63_RS20305 (PIB63_20305) elbB 4154432..4155085 (-) 654 WP_000719791.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=709097 PIB63_RS20270 WP_000809051.1 4149343..4150776(+) (rpoN/rpoN1) [Escherichia coli strain DETEC-E1005]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=709097 PIB63_RS20270 WP_000809051.1 4149343..4150776(+) (rpoN/rpoN1) [Escherichia coli strain DETEC-E1005]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGATACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACACTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTATCAGGGCGAAACGACACAGACCTTACAGGATTACCTGATGTGGCAGGTCGAGTTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATTGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAAAGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465