Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   NLJ82_RS00530 Genome accession   NZ_CP101135
Coordinates   97575..98951 (+) Length   458 a.a.
NCBI ID   WP_001085212.1    Uniprot ID   A0A2B3Y2M8
Organism   Bacillus paranthracis strain Bt C4     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 92575..103951
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NLJ82_RS00510 (NLJ82_00510) ctsR 92768..93229 (+) 462 WP_001244563.1 transcriptional regulator CtsR -
  NLJ82_RS00515 (NLJ82_00515) - 93403..93951 (+) 549 WP_000128382.1 UvrB/UvrC motif-containing protein -
  NLJ82_RS00520 (NLJ82_00520) - 93956..95020 (+) 1065 WP_000050825.1 protein arginine kinase -
  NLJ82_RS00525 (NLJ82_00525) clpC 95043..97478 (+) 2436 WP_000971179.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  NLJ82_RS00530 (NLJ82_00530) radA 97575..98951 (+) 1377 WP_001085212.1 DNA repair protein RadA Machinery gene
  NLJ82_RS00535 (NLJ82_00535) disA 98955..100028 (+) 1074 WP_000392171.1 DNA integrity scanning diadenylate cyclase DisA -
  NLJ82_RS00540 (NLJ82_00540) - 100189..101298 (+) 1110 WP_000919684.1 PIN/TRAM domain-containing protein -
  NLJ82_RS00545 (NLJ82_00545) ispD 101315..101995 (+) 681 WP_000288304.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  NLJ82_RS00550 (NLJ82_00550) ispF 102111..102587 (+) 477 WP_000488386.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49858.44 Da        Isoelectric Point: 7.1421

>NTDB_id=709011 NLJ82_RS00530 WP_001085212.1 97575..98951(+) (radA) [Bacillus paranthracis strain Bt C4]
MAKKKTKFTCQECGYQSPKYMGKCPGCGQWNTLVEEMEPVVSSRRLNYANAIQTEVTKPRRLTEVETKSEARIETKFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADSSYDVLYISGEESAKQIKLRADRLHVNGSNLFVVSETDLQRIA
THIEEVNPAFVVIDSIQTIHLPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKSTAPTDAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRAIIPRKNLGGWTIPDGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=709011 NLJ82_RS00530 WP_001085212.1 97575..98951(+) (radA) [Bacillus paranthracis strain Bt C4]
ATGGCTAAAAAGAAAACAAAATTCACATGTCAAGAGTGTGGGTATCAGTCACCAAAATATATGGGGAAATGCCCTGGATG
TGGTCAATGGAATACGCTTGTTGAAGAGATGGAACCGGTTGTATCATCAAGGCGCCTTAATTATGCGAATGCAATTCAAA
CAGAAGTAACAAAACCAAGACGTCTAACAGAAGTGGAAACAAAGTCTGAGGCGCGTATTGAAACAAAATTTCAAGAATTT
AACCGTGTACTTGGTGGCGGGATTGTAGATGGATCCTTAGTACTGATTGGTGGAGACCCCGGGATTGGAAAATCAACGTT
ACTATTACAAATATCATCACAATTAGCGGATTCTTCATATGATGTCCTATATATATCGGGTGAAGAATCAGCAAAACAGA
TTAAACTGCGTGCGGATCGTTTACATGTAAATGGTAGTAATCTATTTGTTGTATCAGAGACGGACTTACAGCGAATTGCA
ACACATATTGAAGAGGTGAACCCAGCCTTTGTTGTTATTGATTCTATTCAAACAATACATCTACCTGAAGTAACATCAGC
ACCGGGAAGTGTGGCACAAGTTCGTGAATGTACAGCGGAATTAATGAAACTTGCAAAAACGAAAGGAATCCCTATTTTTA
TCGTTGGACATGTGACAAAAGAGGGAGCAATTGCAGGACCTCGTATGTTAGAACATATGGTCGATGCAGTTCTTTACTTT
GAAGGAGACCGTCATCATACATACCGCATTTTGCGAGCAGTAAAGAATCGTTTTGGTTCTACGAATGAAATGGGTATTTT
TGAAATGAAAGAGCTTGGCCTTGCAGAAGTATTAAACCCTTCTGAAATTTTTCTTGAGGAAAGACCAGTTGGGGTTGCAG
GATCAACAGTGGTTGCCTCAATGGAAGGAACAAGACCAGTTTTAGTAGAAATACAAGCATTAATTTCCCCTACTAGTTTT
GGAAACCCTCGAAGAATGGCAACGGGAATTGATCATAACCGTGTATCGCTTATTATGGCAGTGCTAGAAAAAAGAACAGG
CTTATTATTGCAAAATCAGGATGCATATTTAAAAGTAGCAGGTGGTTTGAAGTTAGATGAACCAGCAATTGATTTAGCTG
TCGCTTTAAGTATAGCTTCAAGTTTTAGAGATAAATCTACGGCACCAACCGATGCAGTAATAGGAGAAGTAGGATTAACT
GGAGAAATAAGAAGAGTATCAAGAATTGAACAACGTGTACAAGAAGCAGCTAAATTAGGATTTCAACGCGCTATTATTCC
TAGAAAAAATTTGGGAGGATGGACAATTCCAGATGGGATTGAGGTAGTTGGTGTATCTAATTTAGGAGAAGCACTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(60-222)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2B3Y2M8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.946

100

0.751
  radA Streptococcus mitis NCTC 12261

63.797

98.908

0.631
  radA Streptococcus pneumoniae Rx1

63.576

98.908

0.629
  radA Streptococcus pneumoniae D39

63.576

98.908

0.629
  radA Streptococcus pneumoniae R6

63.576

98.908

0.629
  radA Streptococcus pneumoniae TIGR4

63.576

98.908

0.629
  radA Streptococcus mitis SK321

63.576

98.908

0.629