Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   NGC85_RS02890 Genome accession   NZ_CP101115
Coordinates   600680..601453 (+) Length   257 a.a.
NCBI ID   WP_254805535.1    Uniprot ID   -
Organism   Acinetobacter sp. Z1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 595680..606453
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NGC85_RS02865 (NGC85_02865) - 596579..597865 (+) 1287 WP_254805534.1 glycosyltransferase family 1 protein -
  NGC85_RS02870 (NGC85_02870) - 597891..598442 (+) 552 WP_005056014.1 phosphatase PAP2 family protein -
  NGC85_RS02875 (NGC85_02875) iscX 598537..598737 (+) 201 WP_005056012.1 Fe-S cluster assembly protein IscX -
  NGC85_RS02880 (NGC85_02880) ndk 598846..599277 (+) 432 WP_005056009.1 nucleoside-diphosphate kinase -
  NGC85_RS02885 (NGC85_02885) rlmN 599400..600635 (+) 1236 WP_005061427.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  NGC85_RS02890 (NGC85_02890) pilF 600680..601453 (+) 774 WP_254805535.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  NGC85_RS02895 (NGC85_02895) - 601444..602235 (+) 792 WP_005061429.1 RodZ domain-containing protein -
  NGC85_RS02900 (NGC85_02900) ispG 602258..603373 (+) 1116 WP_004652525.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  NGC85_RS02905 (NGC85_02905) hisS 603370..604662 (+) 1293 WP_254804769.1 histidine--tRNA ligase -
  NGC85_RS02910 (NGC85_02910) - 604694..605392 (+) 699 WP_005061432.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 257 a.a.        Molecular weight: 29183.90 Da        Isoelectric Point: 9.2706

>NTDB_id=708734 NGC85_RS02890 WP_254805535.1 600680..601453(+) (pilF) [Acinetobacter sp. Z1]
MANAVLFTVFGLTACQSTALKKDPEKAVQVRTQLAAEYIRTRDLDSAKRSLDQALSIDSRDANANMMMGILLQQEGSKTN
MDKADAYFKRAISSEPDNAQARNNYGTYLYQLQRYNDAIEQFTRAGATLGYDQRFQSLENLGRIYLKLGDVANAEKSFKQ
ALQANRNATISMLELSEIFYLQQNNRDASQLYEQYVRSVGQKNQGARALWIGIRIARANADQLGMQVLVNQLRALFPDSP
EYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 774 bp        

>NTDB_id=708734 NGC85_RS02890 WP_254805535.1 600680..601453(+) (pilF) [Acinetobacter sp. Z1]
ATAGCAAATGCTGTTTTGTTTACCGTATTCGGCTTAACAGCTTGCCAATCTACTGCATTAAAAAAAGACCCAGAAAAGGC
AGTACAAGTTCGTACACAACTTGCAGCGGAATATATTCGTACACGTGATTTAGATTCGGCTAAACGTTCGCTTGATCAAG
CTTTAAGCATTGATTCGCGTGATGCAAACGCAAATATGATGATGGGAATCTTGCTGCAACAAGAAGGCAGTAAGACCAAT
ATGGACAAAGCGGATGCTTATTTTAAGCGCGCGATTTCCTCTGAACCTGATAATGCACAAGCTCGTAATAATTACGGTAC
ATATTTGTATCAATTGCAACGTTATAATGATGCGATTGAGCAATTTACCCGTGCAGGTGCAACCTTGGGTTATGACCAAC
GCTTTCAATCGTTGGAAAACTTAGGGCGAATCTATTTGAAATTAGGTGATGTTGCGAATGCTGAAAAATCATTCAAACAA
GCGCTACAAGCAAATCGAAATGCAACGATTTCAATGTTAGAGTTGTCTGAAATTTTCTATTTGCAACAAAATAATAGAGA
TGCATCACAACTCTATGAACAATATGTTCGTAGTGTGGGGCAGAAAAACCAAGGTGCACGTGCACTTTGGATTGGTATAC
GCATTGCTCGAGCAAATGCGGATCAATTAGGAATGCAAGTGTTAGTCAACCAGTTGCGTGCTTTATTTCCTGATAGTCCA
GAATATCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(78-98)

Tetratricopeptide repeat (TPR_8)

(30-59)

Tetratricopeptide repeat (TPR_12)

(99-166)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

82.52

95.72

0.79
  pilF Acinetobacter baylyi ADP1

68.872

100

0.689