Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   PIC86_RS02505 Genome accession   NZ_CP116169
Coordinates   521522..522955 (-) Length   477 a.a.
NCBI ID   WP_098737342.1    Uniprot ID   -
Organism   Escherichia coli strain DETEC-P1056     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 516522..527955
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PIC86_RS02470 (PIC86_02470) elbB 517214..517867 (+) 654 WP_001299134.1 isoprenoid biosynthesis glyoxalase ElbB -
  PIC86_RS02475 (PIC86_02475) mtgA 517864..518592 (+) 729 WP_000047091.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  PIC86_RS02480 (PIC86_02480) yrbL 518589..519221 (-) 633 WP_000620387.1 PhoP regulatory network protein YrbL -
  PIC86_RS02485 (PIC86_02485) npr 519434..519706 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  PIC86_RS02490 (PIC86_02490) rapZ 519703..520557 (-) 855 WP_000243741.1 RNase adapter RapZ -
  PIC86_RS02495 (PIC86_02495) ptsN 520603..521094 (-) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  PIC86_RS02500 (PIC86_02500) hpf 521212..521499 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  PIC86_RS02505 (PIC86_02505) rpoN/rpoN1 521522..522955 (-) 1434 WP_098737342.1 RNA polymerase factor sigma-54 Machinery gene
  PIC86_RS02510 (PIC86_02510) lptB 523003..523728 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  PIC86_RS02515 (PIC86_02515) lptA 523735..524292 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  PIC86_RS02520 (PIC86_02520) lptC 524261..524836 (-) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  PIC86_RS02525 (PIC86_02525) kdsC 524833..525399 (-) 567 WP_000030005.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  PIC86_RS02530 (PIC86_02530) kdsD 525420..526406 (-) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  PIC86_RS02535 (PIC86_02535) yrbG 526420..527397 (-) 978 WP_000922872.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53992.81 Da        Isoelectric Point: 4.3837

>NTDB_id=708637 PIC86_RS02505 WP_098737342.1 521522..522955(-) (rpoN/rpoN1) [Escherichia coli strain DETEC-P1056]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEINTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=708637 PIC86_RS02505 WP_098737342.1 521522..522955(-) (rpoN/rpoN1) [Escherichia coli strain DETEC-P1056]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCAACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
TGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATCGACATCGACGAGGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTAGCGGCAAAAGATCTGCGTGACTGCCTGCTAATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465