Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   PIC30_RS02625 Genome accession   NZ_CP116071
Coordinates   539398..540831 (-) Length   477 a.a.
NCBI ID   WP_097494566.1    Uniprot ID   -
Organism   Escherichia coli strain DETEC-S589     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 534398..545831
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PIC30_RS02590 (PIC30_02590) elbB 535089..535742 (+) 654 WP_000719794.1 isoprenoid biosynthesis glyoxalase ElbB -
  PIC30_RS02595 (PIC30_02595) mtgA 535739..536467 (+) 729 WP_000047069.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  PIC30_RS02600 (PIC30_02600) yrbL 536464..537096 (-) 633 WP_000620407.1 PhoP regulatory network protein YrbL -
  PIC30_RS02605 (PIC30_02605) npr 537310..537582 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  PIC30_RS02610 (PIC30_02610) rapZ 537579..538433 (-) 855 WP_000243741.1 RNase adapter RapZ -
  PIC30_RS02615 (PIC30_02615) ptsN 538479..538970 (-) 492 WP_000183674.1 PTS IIA-like nitrogen regulatory protein PtsN -
  PIC30_RS02620 (PIC30_02620) hpf 539088..539375 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  PIC30_RS02625 (PIC30_02625) rpoN/rpoN1 539398..540831 (-) 1434 WP_097494566.1 RNA polymerase factor sigma-54 Machinery gene
  PIC30_RS02630 (PIC30_02630) lptB 540879..541604 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  PIC30_RS02635 (PIC30_02635) lptA 541611..542168 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  PIC30_RS02640 (PIC30_02640) lptC 542137..542712 (-) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  PIC30_RS02645 (PIC30_02645) kdsC 542709..543275 (-) 567 WP_000030016.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  PIC30_RS02650 (PIC30_02650) kdsD 543296..544282 (-) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  PIC30_RS02655 (PIC30_02655) yrbG 544296..545273 (-) 978 WP_000922880.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53965.73 Da        Isoelectric Point: 4.3380

>NTDB_id=707323 PIC30_RS02625 WP_097494566.1 539398..540831(-) (rpoN/rpoN1) [Escherichia coli strain DETEC-S589]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQQFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=707323 PIC30_RS02625 WP_097494566.1 539398..540831(-) (rpoN/rpoN1) [Escherichia coli strain DETEC-S589]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGCAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACACTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTATCAGGGCGAAACGACACAGACCTTACAGGATTACCTGATGTGGCAGGTCGAGTTGACACCATTTT
CCGACACTGACCGCGCGATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATTGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACAGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGCCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCATGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTATTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTTTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGATAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGCGAAGAATATATGAAACCGATGGTACTGGCTGATATCGCCCAGGCCG
TCGAGATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACTGAAGGCGGGGGTGAAGCCTCTTCCACAGCGATTCGCGCACTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465