Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   PID00_RS14285 Genome accession   NZ_CP116067
Coordinates   2826898..2828331 (+) Length   477 a.a.
NCBI ID   WP_096121882.1    Uniprot ID   -
Organism   Escherichia coli strain DETEC-S792     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2821898..2833331
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PID00_RS14255 (PID00_14255) yrbG 2822456..2823433 (+) 978 WP_096121883.1 calcium/sodium antiporter -
  PID00_RS14260 (PID00_14260) kdsD 2823447..2824433 (+) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  PID00_RS14265 (PID00_14265) kdsC 2824454..2825020 (+) 567 WP_053290523.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  PID00_RS14270 (PID00_14270) lptC 2825017..2825592 (+) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  PID00_RS14275 (PID00_14275) lptA 2825561..2826118 (+) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  PID00_RS14280 (PID00_14280) lptB 2826125..2826850 (+) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  PID00_RS14285 (PID00_14285) rpoN/rpoN1 2826898..2828331 (+) 1434 WP_096121882.1 RNA polymerase factor sigma-54 Machinery gene
  PID00_RS14290 (PID00_14290) hpf 2828354..2828641 (+) 288 WP_001176599.1 ribosome hibernation promoting factor -
  PID00_RS14295 (PID00_14295) ptsN 2828759..2829250 (+) 492 WP_000183674.1 PTS IIA-like nitrogen regulatory protein PtsN -
  PID00_RS14300 (PID00_14300) rapZ 2829296..2830150 (+) 855 WP_000243741.1 RNase adapter RapZ -
  PID00_RS14305 (PID00_14305) npr 2830147..2830419 (+) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  PID00_RS14310 (PID00_14310) yrbL 2830632..2831264 (+) 633 WP_000620405.1 PhoP regulatory network protein YrbL -
  PID00_RS14315 (PID00_14315) mtgA 2831261..2831989 (-) 729 WP_000047075.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  PID00_RS14320 (PID00_14320) elbB 2831986..2832639 (-) 654 WP_001300411.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53992.85 Da        Isoelectric Point: 4.4085

>NTDB_id=707263 PID00_RS14285 WP_096121882.1 2826898..2828331(+) (rpoN/rpoN1) [Escherichia coli strain DETEC-S792]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPK
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=707263 PID00_RS14285 WP_096121882.1 2826898..2828331(+) (rpoN/rpoN1) [Escherichia coli strain DETEC-S792]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACACTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGAAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTATCAGGGCGAAACGACACAGACCTTACAGGATTACCTGATGTGGCAGGTCGAGTTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATTGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGCCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCATGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTATTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGATAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGCGAAGAATATATGAAACCGATGGTACTGGCTGATATCGCCCAGGCCG
TCGAGATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAA
TATTTCTTTTCCAGTCACGTCAATACTGAAGGCGGGGGTGAAGCCTCTTCCACAGCGATTCGCGCACTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCATTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CTCGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465