Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   NMD73_RS13790 Genome accession   NZ_CP100846
Coordinates   2987404..2988873 (+) Length   489 a.a.
NCBI ID   WP_370549230.1    Uniprot ID   -
Organism   Edwardsiella tarda strain GT30     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2982404..2993873
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD73_RS13760 (NMD73_13720) - 2982833..2983822 (+) 990 WP_370549229.1 calcium/sodium antiporter -
  NMD73_RS13765 (NMD73_13725) kdsD 2983876..2984862 (+) 987 WP_205438784.1 arabinose-5-phosphate isomerase KdsD -
  NMD73_RS13770 (NMD73_13730) kdsC 2984878..2985444 (+) 567 WP_205438785.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  NMD73_RS13775 (NMD73_13735) lptC 2985441..2986013 (+) 573 WP_205438786.1 LPS export ABC transporter periplasmic protein LptC -
  NMD73_RS13780 (NMD73_13740) lptA 2985988..2986545 (+) 558 WP_205438787.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  NMD73_RS13785 (NMD73_13745) lptB 2986552..2987277 (+) 726 WP_005281703.1 LPS export ABC transporter ATP-binding protein -
  NMD73_RS13790 (NMD73_13750) rpoN/rpoN1 2987404..2988873 (+) 1470 WP_370549230.1 RNA polymerase factor sigma-54 Machinery gene
  NMD73_RS13795 (NMD73_13755) hpf 2988895..2989182 (+) 288 WP_024524583.1 ribosome hibernation promoting factor -
  NMD73_RS13800 (NMD73_13760) ptsN 2989317..2989763 (+) 447 WP_005297331.1 PTS IIA-like nitrogen regulatory protein PtsN -
  NMD73_RS13805 (NMD73_13765) rapZ 2989871..2990722 (+) 852 WP_005281696.1 RNase adapter RapZ -
  NMD73_RS13810 (NMD73_13770) npr 2990730..2991002 (+) 273 WP_205438789.1 PTS phosphocarrier protein NPr -
  NMD73_RS13815 (NMD73_13775) rnk 2991179..2991589 (+) 411 WP_370549231.1 nucleoside diphosphate kinase regulator -
  NMD73_RS13820 (NMD73_13780) - 2991621..2992136 (-) 516 WP_205438791.1 cytochrome b/b6 domain-containing protein -
  NMD73_RS13825 (NMD73_13785) cybC 2992142..2992528 (-) 387 WP_205438792.1 cytochrome b562 -
  NMD73_RS13830 (NMD73_13790) deoD 2992901..2993626 (-) 726 WP_005281692.1 purine-nucleoside phosphorylase -

Sequence

Protein


Download         Length: 489 a.a.        Molecular weight: 55004.83 Da        Isoelectric Point: 4.3808

>NTDB_id=707229 NMD73_RS13790 WP_370549230.1 2987404..2988873(+) (rpoN/rpoN1) [Edwardsiella tarda strain GT30]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQEIQQALESNPLLEQLDPNEEAGDDGVVLESTQEDAVTRDDADPLD
SREALEQREMPDELPLDASWDEIYTAGTPSGTGTDYHDDELPVFQGETTQTLQDYLMWQVELTPFSDTDLAIATAIVDAV
DDTGYLSASLEEILESLGDDRVLPEEIEAVLKRIQRFDPVGVAARDLRDCLLVQLSQFADDTPYLNEARSIVDRHLDLLG
NHDFRNLMRSTKLKEEVLKAAMQLIQNLDPRPGLSINTGESDYVIPDVLVHKHQERWVVELNQDSVPRLRINQHYAALGT
QARNDSDSQFIRSNLQEAKWLIKSLESRNDTLLKVSRCIVEQQQAFFEHGPEAMKPMVLADIAQQVEMHESTISRVTTQK
FLHSPRGIFELKYFFSSHVSTEDGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTLLADQGIMVARRTVAKYRESLSIP
PSNQRKQLV

Nucleotide


Download         Length: 1470 bp        

>NTDB_id=707229 NMD73_RS13790 WP_370549230.1 2987404..2988873(+) (rpoN/rpoN1) [Edwardsiella tarda strain GT30]
ATGAAGCAAGGCTTGCAACTCAGACTCAGCCAGCAGCTGGCCATGACGCCCCAACTGCAGCAGGCCATTCGCCTGTTGCA
GCTCTCGACGCTGGAGTTACAACAAGAGATCCAGCAGGCGTTGGAAAGCAATCCTCTGCTGGAACAACTGGATCCCAATG
AGGAAGCCGGCGACGACGGCGTCGTTCTCGAGAGTACACAGGAGGATGCCGTCACACGCGATGACGCCGATCCACTGGAT
AGCCGTGAGGCGTTGGAGCAACGAGAGATGCCGGACGAGCTGCCGCTAGATGCCAGCTGGGACGAGATCTATACCGCTGG
CACCCCCTCTGGCACGGGAACGGACTATCATGACGACGAGTTACCCGTATTTCAGGGAGAGACGACCCAAACGCTGCAGG
ACTATCTGATGTGGCAGGTCGAACTGACCCCCTTCAGTGATACCGATCTGGCCATCGCTACCGCCATCGTCGATGCCGTC
GACGATACCGGCTATCTCAGCGCCTCGTTGGAGGAGATCCTCGAGAGTCTGGGGGATGATCGCGTCCTACCGGAAGAGAT
CGAGGCCGTCCTCAAGCGCATCCAACGTTTTGACCCGGTCGGTGTCGCCGCGCGTGATCTGCGCGACTGCCTACTGGTCC
AGCTCTCGCAATTCGCCGACGACACCCCCTATCTCAACGAGGCTCGTAGCATCGTCGATCGCCACCTCGATCTGTTGGGT
AATCACGATTTCCGAAATCTGATGCGTTCGACTAAGCTAAAAGAAGAGGTGCTCAAGGCGGCGATGCAACTGATTCAGAA
CCTCGACCCGCGGCCGGGACTCTCCATCAATACCGGCGAGTCTGACTATGTCATCCCCGACGTGCTGGTGCATAAACATC
AGGAACGCTGGGTGGTTGAGCTCAATCAGGACAGCGTCCCGCGCCTGCGTATCAACCAACATTATGCCGCGCTGGGCACT
CAGGCGCGTAACGACAGCGACAGCCAGTTCATTCGCAGCAATCTGCAAGAGGCGAAGTGGCTGATCAAGAGTCTAGAGAG
CCGTAACGATACCCTGCTGAAGGTGTCGCGTTGCATCGTGGAGCAGCAGCAAGCGTTCTTCGAGCACGGGCCGGAGGCGA
TGAAGCCGATGGTGCTGGCAGATATCGCTCAGCAAGTCGAAATGCACGAGTCGACGATCTCACGCGTGACTACGCAGAAG
TTTCTGCACAGCCCGCGCGGAATCTTTGAACTGAAATATTTCTTCTCCAGCCATGTCAGCACCGAGGACGGCGGCGAGGC
CTCTTCCACGGCCATCCGAGCGCTGGTGAAGAAACTGATCGCGGCAGAAAATCCGGCGAAGCCGCTGAGTGATAGCAAAC
TTACCACTCTATTGGCGGATCAGGGTATCATGGTCGCCCGGCGTACTGTCGCAAAATACCGAGAGTCTTTGTCCATTCCA
CCGTCCAATCAGCGTAAACAGCTGGTGTGA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(329-487)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(128-315)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.367

100

0.481