Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PIH14_RS12805 Genome accession   NZ_CP116046
Coordinates   2614365..2615861 (-) Length   498 a.a.
NCBI ID   WP_001040683.1    Uniprot ID   -
Organism   Escherichia coli strain EC812A1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2609365..2620861
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PIH14_RS12780 (PIH14_12785) panM 2609587..2609970 (+) 384 WP_000778792.1 aspartate 1-decarboxylase autocleavage activator PanM -
  PIH14_RS12785 (PIH14_12790) livJ 2610170..2611273 (-) 1104 WP_001022007.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  PIH14_RS12790 (PIH14_12795) rpoH 2611544..2612398 (-) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  PIH14_RS12795 (PIH14_12800) ftsX 2612643..2613701 (-) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  PIH14_RS12800 (PIH14_12805) ftsE 2613694..2614362 (-) 669 WP_000617726.1 cell division ATP-binding protein FtsE -
  PIH14_RS12805 (PIH14_12810) pilA 2614365..2615861 (-) 1497 WP_001040683.1 signal recognition particle-docking protein FtsY Machinery gene
  PIH14_RS12810 (PIH14_12815) rsmD 2616011..2616607 (+) 597 WP_000743181.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  PIH14_RS12815 (PIH14_12820) yhhL 2616597..2616866 (+) 270 WP_001295207.1 DUF1145 family protein -
  PIH14_RS12820 (PIH14_12825) yhhM 2616869..2617228 (-) 360 WP_000042900.1 DUF2500 domain-containing protein -
  PIH14_RS12825 (PIH14_12830) yhhN 2617369..2617995 (+) 627 WP_000964724.1 lysoplasmalogenase -
  PIH14_RS12830 (PIH14_12835) zntA 2618069..2620267 (+) 2199 WP_000106560.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  PIH14_RS12835 (PIH14_12840) tusA 2620369..2620614 (-) 246 WP_000130621.1 sulfurtransferase TusA -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54617.41 Da        Isoelectric Point: 4.1560

>NTDB_id=707189 PIH14_RS12805 WP_001040683.1 2614365..2615861(-) (pilA) [Escherichia coli strain EC812A1]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQSVVEEIVQVQEPAKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAGVVAQPEPVVEETPEPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=707189 PIH14_RS12805 WP_001040683.1 2614365..2615861(-) (pilA) [Escherichia coli strain EC812A1]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAATCGGTTGTAGAAGAAATCGTTCAGGTGCAAGAGCCTGCGAAGGCTTCTGAACAAGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGGAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCGGTGATGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTTTTGATCGCCGAT
GTGGGCGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCACGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCGGGCGATACTTTCCGTGCAGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCAGCTAAAG
CGCGTAATATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATCGATGCCAGCACCGGGCAGAA
CGCGGTAAGCCAGGCCAAACTGTTCCATGAAGCCGTTGGTTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)

SRP54-type protein, GTPase domain (SRP54)

(294-494)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.831

77.309

0.378