Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   NMD76_RS14020 Genome accession   NZ_CP100831
Coordinates   3023678..3025147 (+) Length   489 a.a.
NCBI ID   WP_370549230.1    Uniprot ID   -
Organism   Edwardsiella tarda strain GT50     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3018678..3030147
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD76_RS13990 (NMD76_13920) - 3019107..3020096 (+) 990 WP_370549229.1 calcium/sodium antiporter -
  NMD76_RS13995 (NMD76_13925) kdsD 3020150..3021136 (+) 987 WP_205438784.1 arabinose-5-phosphate isomerase KdsD -
  NMD76_RS14000 (NMD76_13930) kdsC 3021152..3021718 (+) 567 WP_205438785.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  NMD76_RS14005 (NMD76_13935) lptC 3021715..3022287 (+) 573 WP_205438786.1 LPS export ABC transporter periplasmic protein LptC -
  NMD76_RS14010 (NMD76_13940) lptA 3022262..3022819 (+) 558 WP_205438787.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  NMD76_RS14015 (NMD76_13945) lptB 3022826..3023551 (+) 726 WP_005281703.1 LPS export ABC transporter ATP-binding protein -
  NMD76_RS14020 (NMD76_13950) rpoN/rpoN1 3023678..3025147 (+) 1470 WP_370549230.1 RNA polymerase factor sigma-54 Machinery gene
  NMD76_RS14025 (NMD76_13955) hpf 3025169..3025456 (+) 288 WP_024524583.1 ribosome hibernation promoting factor -
  NMD76_RS14030 (NMD76_13960) ptsN 3025591..3026037 (+) 447 WP_005297331.1 PTS IIA-like nitrogen regulatory protein PtsN -
  NMD76_RS14035 (NMD76_13965) rapZ 3026145..3026996 (+) 852 WP_005281696.1 RNase adapter RapZ -
  NMD76_RS14040 (NMD76_13970) npr 3027004..3027276 (+) 273 WP_205438789.1 PTS phosphocarrier protein NPr -
  NMD76_RS14045 (NMD76_13975) rnk 3027453..3027863 (+) 411 WP_370549231.1 nucleoside diphosphate kinase regulator -
  NMD76_RS14050 (NMD76_13980) - 3027895..3028410 (-) 516 WP_205438791.1 cytochrome b/b6 domain-containing protein -
  NMD76_RS14055 (NMD76_13985) cybC 3028416..3028802 (-) 387 WP_205438792.1 cytochrome b562 -
  NMD76_RS14060 (NMD76_13990) deoD 3029175..3029900 (-) 726 WP_005281692.1 purine-nucleoside phosphorylase -

Sequence

Protein


Download         Length: 489 a.a.        Molecular weight: 55004.83 Da        Isoelectric Point: 4.3808

>NTDB_id=707189 NMD76_RS14020 WP_370549230.1 3023678..3025147(+) (rpoN/rpoN1) [Edwardsiella tarda strain GT50]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQEIQQALESNPLLEQLDPNEEAGDDGVVLESTQEDAVTRDDADPLD
SREALEQREMPDELPLDASWDEIYTAGTPSGTGTDYHDDELPVFQGETTQTLQDYLMWQVELTPFSDTDLAIATAIVDAV
DDTGYLSASLEEILESLGDDRVLPEEIEAVLKRIQRFDPVGVAARDLRDCLLVQLSQFADDTPYLNEARSIVDRHLDLLG
NHDFRNLMRSTKLKEEVLKAAMQLIQNLDPRPGLSINTGESDYVIPDVLVHKHQERWVVELNQDSVPRLRINQHYAALGT
QARNDSDSQFIRSNLQEAKWLIKSLESRNDTLLKVSRCIVEQQQAFFEHGPEAMKPMVLADIAQQVEMHESTISRVTTQK
FLHSPRGIFELKYFFSSHVSTEDGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTLLADQGIMVARRTVAKYRESLSIP
PSNQRKQLV

Nucleotide


Download         Length: 1470 bp        

>NTDB_id=707189 NMD76_RS14020 WP_370549230.1 3023678..3025147(+) (rpoN/rpoN1) [Edwardsiella tarda strain GT50]
ATGAAGCAAGGCTTGCAACTCAGACTCAGCCAGCAGCTGGCCATGACGCCCCAACTGCAGCAGGCCATTCGCCTGTTGCA
GCTCTCGACGCTGGAGTTACAACAAGAGATCCAGCAGGCGTTGGAAAGCAATCCTCTGCTGGAACAACTGGATCCCAATG
AGGAAGCCGGCGACGACGGCGTCGTTCTCGAGAGTACACAGGAGGATGCCGTCACACGCGATGACGCCGATCCACTGGAT
AGCCGTGAGGCGTTGGAGCAACGAGAGATGCCGGACGAGCTGCCGCTAGATGCCAGCTGGGACGAGATCTATACCGCTGG
CACCCCCTCTGGCACGGGAACGGACTATCATGACGACGAGTTACCCGTATTTCAGGGAGAGACGACCCAAACGCTGCAGG
ACTATCTGATGTGGCAGGTCGAACTGACCCCCTTCAGTGATACCGATCTGGCCATCGCTACCGCCATCGTCGATGCCGTC
GACGATACCGGCTATCTCAGCGCCTCGTTGGAGGAGATCCTCGAGAGTCTGGGGGATGATCGCGTCCTACCGGAAGAGAT
CGAGGCCGTCCTCAAGCGCATCCAACGTTTTGACCCGGTCGGTGTCGCCGCGCGTGATCTGCGCGACTGCCTACTGGTCC
AGCTCTCGCAATTCGCCGACGACACCCCCTATCTCAACGAGGCTCGTAGCATCGTCGATCGCCACCTCGATCTGTTGGGT
AATCACGATTTCCGAAATCTGATGCGTTCGACTAAGCTAAAAGAAGAGGTGCTCAAGGCGGCGATGCAACTGATTCAGAA
CCTCGACCCGCGGCCGGGACTCTCCATCAATACCGGCGAGTCTGACTATGTCATCCCCGACGTGCTGGTGCATAAACATC
AGGAACGCTGGGTGGTTGAGCTCAATCAGGACAGCGTCCCGCGCCTGCGTATCAACCAACATTATGCCGCGCTGGGCACT
CAGGCGCGTAACGACAGCGACAGCCAGTTCATTCGCAGCAATCTGCAAGAGGCGAAGTGGCTGATCAAGAGTCTAGAGAG
CCGTAACGATACCCTGCTGAAGGTGTCGCGTTGCATCGTGGAGCAGCAGCAAGCGTTCTTCGAGCACGGGCCGGAGGCGA
TGAAGCCGATGGTGCTGGCAGATATCGCTCAGCAAGTCGAAATGCACGAGTCGACGATCTCACGCGTGACTACGCAGAAG
TTTCTGCACAGCCCGCGCGGAATCTTTGAACTGAAATATTTCTTCTCCAGCCATGTCAGCACCGAGGACGGCGGCGAGGC
CTCTTCCACGGCCATCCGAGCGCTGGTGAAGAAACTGATCGCGGCAGAAAATCCGGCGAAGCCGCTGAGTGATAGCAAAC
TTACCACTCTATTGGCGGATCAGGGTATCATGGTCGCCCGGCGTACTGTCGCAAAATACCGAGAGTCTTTGTCCATTCCA
CCGTCCAATCAGCGTAAACAGCTGGTGTGA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(329-487)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(128-315)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.367

100

0.481