Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   PIH14_RS11510 Genome accession   NZ_CP116046
Coordinates   2355374..2356807 (+) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli strain EC812A1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2350374..2361807
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PIH14_RS11480 (PIH14_11480) yrbG 2350932..2351909 (+) 978 WP_000922901.1 calcium/sodium antiporter -
  PIH14_RS11485 (PIH14_11485) kdsD 2351923..2352909 (+) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  PIH14_RS11490 (PIH14_11490) kdsC 2352930..2353496 (+) 567 WP_000030005.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  PIH14_RS11495 (PIH14_11495) lptC 2353493..2354068 (+) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  PIH14_RS11500 (PIH14_11500) lptA 2354037..2354594 (+) 558 WP_112958405.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  PIH14_RS11505 (PIH14_11505) lptB 2354601..2355326 (+) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  PIH14_RS11510 (PIH14_11510) rpoN/rpoN1 2355374..2356807 (+) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  PIH14_RS11515 (PIH14_11515) hpf 2356830..2357117 (+) 288 WP_001176599.1 ribosome hibernation promoting factor -
  PIH14_RS11520 (PIH14_11520) ptsN 2357235..2357726 (+) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  PIH14_RS11525 (PIH14_11525) rapZ 2357772..2358626 (+) 855 WP_000243741.1 RNase adapter RapZ -
  PIH14_RS11530 (PIH14_11530) npr 2358623..2358895 (+) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  PIH14_RS11535 (PIH14_11535) yrbL 2359108..2359740 (+) 633 WP_000620410.1 PhoP regulatory network protein YrbL -
  PIH14_RS11540 (PIH14_11540) mtgA 2359737..2360465 (-) 729 WP_000047091.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  PIH14_RS11545 (PIH14_11545) elbB 2360462..2361115 (-) 654 WP_001300411.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=707182 PIH14_RS11510 WP_000809051.1 2355374..2356807(+) (rpoN/rpoN1) [Escherichia coli strain EC812A1]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=707182 PIH14_RS11510 WP_000809051.1 2355374..2356807(+) (rpoN/rpoN1) [Escherichia coli strain EC812A1]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
TGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATCGACATCGACGAGGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTAGCGGCAAAAGATCTGCGTGACTGCCTGCTAATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGATTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACAGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCTCGCAATGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGCGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAACTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465