Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   NMD80_RS14960 Genome accession   NZ_CP100817
Coordinates   3145075..3146544 (+) Length   489 a.a.
NCBI ID   WP_370553739.1    Uniprot ID   -
Organism   Edwardsiella tarda strain GT63     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3140075..3151544
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD80_RS14930 (NMD80_14900) - 3140505..3141494 (+) 990 WP_078000403.1 calcium/sodium antiporter -
  NMD80_RS14935 (NMD80_14905) kdsD 3141547..3142533 (+) 987 WP_370558877.1 arabinose-5-phosphate isomerase KdsD -
  NMD80_RS14940 (NMD80_14910) kdsC 3142549..3143115 (+) 567 WP_035604658.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  NMD80_RS14945 (NMD80_14915) lptC 3143112..3143684 (+) 573 WP_035604656.1 LPS export ABC transporter periplasmic protein LptC -
  NMD80_RS14950 (NMD80_14920) lptA 3143659..3144216 (+) 558 WP_005281704.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  NMD80_RS14955 (NMD80_14925) lptB 3144223..3144948 (+) 726 WP_005281703.1 LPS export ABC transporter ATP-binding protein -
  NMD80_RS14960 (NMD80_14930) rpoN/rpoN1 3145075..3146544 (+) 1470 WP_370553739.1 RNA polymerase factor sigma-54 Machinery gene
  NMD80_RS14965 (NMD80_14935) hpf 3146566..3146853 (+) 288 WP_024524583.1 ribosome hibernation promoting factor -
  NMD80_RS14970 (NMD80_14940) ptsN 3146988..3147434 (+) 447 WP_005297331.1 PTS IIA-like nitrogen regulatory protein PtsN -
  NMD80_RS14975 (NMD80_14945) rapZ 3147542..3148393 (+) 852 WP_005281696.1 RNase adapter RapZ -
  NMD80_RS14980 (NMD80_14950) npr 3148401..3148673 (+) 273 WP_005281695.1 PTS phosphocarrier protein NPr -
  NMD80_RS14985 (NMD80_14955) rnk 3148850..3149260 (+) 411 WP_370558878.1 nucleoside diphosphate kinase regulator -
  NMD80_RS14990 (NMD80_14960) - 3149296..3149799 (-) 504 WP_035604652.1 cytochrome b/b6 domain-containing protein -
  NMD80_RS14995 (NMD80_14965) cybC 3149805..3150191 (-) 387 WP_035604649.1 cytochrome b562 -
  NMD80_RS15000 (NMD80_14970) deoD 3150406..3151131 (-) 726 WP_005281692.1 purine-nucleoside phosphorylase -

Sequence

Protein


Download         Length: 489 a.a.        Molecular weight: 55017.87 Da        Isoelectric Point: 4.4249

>NTDB_id=707109 NMD80_RS14960 WP_370553739.1 3145075..3146544(+) (rpoN/rpoN1) [Edwardsiella tarda strain GT63]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQEIQQALESNPLLEQLDPNEEAGDDGVVRENTQEDVVTRDDADPLD
SREALEQREMPDELPLDASWDEIYTAGTPSGTGTDYHDDELPVFQGETTQTLQDYLMWQVELTPFSDTDLAIATAIVDAV
DDTGYLSASLAEILESLGDDRVLPEEIEAVLKRIQRFDPVGVAARDLRDCLLVQLSQFADDTPYLNEARSIVDRHLDLLG
NHDFRNLMRSTKLKEEVLKAAMQLIQSLDPRPGLSINTGESDYVIPDVLVHKHQERWVVELNQDSVPRLRINQHYAALGT
QARNDSDSQFIRSNLQEAKWLIKSLESRNDTLLKVSRCIVEQQQAFFEHGPEAMKPMVLADIAQQVEMHESTISRVTTQK
FLHSPRGIFELKYFFSSHVSTEDGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTLLADQGIMVARRTVAKYRESLSIP
PSNQRKQLV

Nucleotide


Download         Length: 1470 bp        

>NTDB_id=707109 NMD80_RS14960 WP_370553739.1 3145075..3146544(+) (rpoN/rpoN1) [Edwardsiella tarda strain GT63]
ATGAAGCAAGGCTTGCAACTCAGACTCAGCCAGCAGCTGGCCATGACGCCCCAACTGCAACAGGCCATTCGCCTGTTGCA
GCTCTCGACGCTAGAGTTACAGCAAGAGATCCAGCAGGCGTTGGAAAGCAATCCTCTGCTGGAACAACTGGATCCCAATG
AGGAAGCCGGCGACGACGGCGTCGTTCGCGAGAATACACAGGAGGATGTCGTCACACGCGATGATGCCGATCCACTGGAT
AGCCGCGAGGCGTTGGAGCAACGTGAGATGCCGGACGAGCTGCCGCTGGATGCCAGCTGGGACGAGATCTATACCGCTGG
CACCCCCTCAGGCACGGGAACGGATTATCATGACGACGAGTTACCCGTGTTTCAGGGAGAGACGACCCAAACGCTGCAGG
ACTATCTGATGTGGCAGGTCGAACTGACCCCCTTCAGTGATACCGATCTGGCCATCGCTACCGCCATCGTCGATGCCGTC
GACGATACCGGCTATCTCAGCGCCTCGTTGGCGGAGATCCTCGAGAGTCTAGGGGACGATCGCGTCCTACCGGAAGAGAT
CGAGGCCGTCCTCAAGCGCATCCAACGTTTTGATCCGGTCGGTGTCGCCGCGCGTGATCTGCGCGACTGCCTACTGGTCC
AGCTCTCGCAATTCGCCGACGACACGCCCTATCTCAACGAGGCTCGTAGCATCGTCGATCGCCACCTCGATCTGTTGGGT
AATCACGATTTCCGAAATCTGATGCGTTCGACTAAGCTAAAAGAAGAGGTGCTCAAGGCGGCGATGCAACTGATCCAGAG
CCTCGACCCGCGGCCAGGACTCTCCATCAATACCGGCGAGTCTGACTACGTCATCCCCGACGTGCTGGTGCATAAACATC
AGGAGCGCTGGGTGGTTGAGCTCAATCAGGACAGCGTCCCGCGCCTGCGTATCAACCAACATTACGCCGCGCTGGGCACT
CAGGCGCGTAACGACAGCGACAGCCAGTTCATTCGCAGCAATCTGCAAGAAGCGAAGTGGCTGATCAAGAGCCTAGAGAG
CCGTAACGATACCCTGCTGAAGGTATCGCGTTGCATCGTAGAGCAGCAGCAAGCGTTCTTCGAGCACGGGCCGGAGGCGA
TGAAGCCGATGGTGCTGGCAGATATCGCTCAGCAAGTCGAAATGCACGAATCGACGATCTCACGTGTGACTACGCAGAAG
TTTCTGCACAGCCCGCGCGGAATCTTTGAACTGAAATATTTCTTCTCCAGCCATGTCAGCACCGAGGACGGCGGCGAGGC
CTCCTCCACGGCCATCCGGGCGCTGGTGAAGAAACTGATCGCGGCAGAAAATCCGGCGAAACCGCTGAGTGATAGCAAAC
TTACCACTCTATTGGCGGATCAGGGTATCATGGTCGCCCGGCGTACTGTCGCAAAATACCGAGAGTCTTTGTCCATTCCA
CCGTCCAATCAGCGTAAACAGCTGGTGTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(128-315)

Sigma-54, DNA binding domain (Sigma54_DBD)

(329-487)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.367

100

0.481