Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   NMD81_RS13535 Genome accession   NZ_CP100814
Coordinates   2973025..2974494 (+) Length   489 a.a.
NCBI ID   WP_370553739.1    Uniprot ID   -
Organism   Edwardsiella tarda strain GT64     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2968025..2979494
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD81_RS13505 (NMD81_13495) - 2968455..2969444 (+) 990 WP_078000403.1 calcium/sodium antiporter -
  NMD81_RS13510 (NMD81_13500) kdsD 2969497..2970483 (+) 987 WP_078000404.1 arabinose-5-phosphate isomerase KdsD -
  NMD81_RS13515 (NMD81_13505) kdsC 2970499..2971065 (+) 567 WP_035604658.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  NMD81_RS13520 (NMD81_13510) lptC 2971062..2971634 (+) 573 WP_035604656.1 LPS export ABC transporter periplasmic protein LptC -
  NMD81_RS13525 (NMD81_13515) lptA 2971609..2972166 (+) 558 WP_005281704.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  NMD81_RS13530 (NMD81_13520) lptB 2972173..2972898 (+) 726 WP_005281703.1 LPS export ABC transporter ATP-binding protein -
  NMD81_RS13535 (NMD81_13525) rpoN/rpoN1 2973025..2974494 (+) 1470 WP_370553739.1 RNA polymerase factor sigma-54 Machinery gene
  NMD81_RS13540 (NMD81_13530) hpf 2974516..2974803 (+) 288 WP_024524583.1 ribosome hibernation promoting factor -
  NMD81_RS13545 (NMD81_13535) ptsN 2974938..2975384 (+) 447 WP_005297331.1 PTS IIA-like nitrogen regulatory protein PtsN -
  NMD81_RS13550 (NMD81_13540) rapZ 2975492..2976343 (+) 852 WP_005281696.1 RNase adapter RapZ -
  NMD81_RS13555 (NMD81_13545) npr 2976351..2976623 (+) 273 WP_005281695.1 PTS phosphocarrier protein NPr -
  NMD81_RS13560 (NMD81_13550) rnk 2976800..2977210 (+) 411 WP_370553740.1 nucleoside diphosphate kinase regulator -
  NMD81_RS13565 (NMD81_13555) - 2977246..2977749 (-) 504 WP_035604652.1 cytochrome b/b6 domain-containing protein -
  NMD81_RS13570 (NMD81_13560) cybC 2977755..2978141 (-) 387 WP_035604649.1 cytochrome b562 -
  NMD81_RS13575 (NMD81_13565) deoD 2978356..2979081 (-) 726 WP_005281692.1 purine-nucleoside phosphorylase -

Sequence

Protein


Download         Length: 489 a.a.        Molecular weight: 55017.87 Da        Isoelectric Point: 4.4249

>NTDB_id=707087 NMD81_RS13535 WP_370553739.1 2973025..2974494(+) (rpoN/rpoN1) [Edwardsiella tarda strain GT64]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQEIQQALESNPLLEQLDPNEEAGDDGVVRENTQEDVVTRDDADPLD
SREALEQREMPDELPLDASWDEIYTAGTPSGTGTDYHDDELPVFQGETTQTLQDYLMWQVELTPFSDTDLAIATAIVDAV
DDTGYLSASLAEILESLGDDRVLPEEIEAVLKRIQRFDPVGVAARDLRDCLLVQLSQFADDTPYLNEARSIVDRHLDLLG
NHDFRNLMRSTKLKEEVLKAAMQLIQSLDPRPGLSINTGESDYVIPDVLVHKHQERWVVELNQDSVPRLRINQHYAALGT
QARNDSDSQFIRSNLQEAKWLIKSLESRNDTLLKVSRCIVEQQQAFFEHGPEAMKPMVLADIAQQVEMHESTISRVTTQK
FLHSPRGIFELKYFFSSHVSTEDGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTLLADQGIMVARRTVAKYRESLSIP
PSNQRKQLV

Nucleotide


Download         Length: 1470 bp        

>NTDB_id=707087 NMD81_RS13535 WP_370553739.1 2973025..2974494(+) (rpoN/rpoN1) [Edwardsiella tarda strain GT64]
ATGAAGCAAGGCTTGCAACTCAGACTCAGCCAGCAGCTGGCCATGACGCCCCAACTGCAACAGGCCATTCGCCTGTTGCA
GCTCTCGACGCTAGAGTTACAGCAAGAGATCCAGCAGGCGTTGGAAAGCAATCCTCTGCTGGAACAACTGGATCCCAATG
AGGAAGCCGGCGACGACGGCGTCGTTCGCGAGAATACACAGGAGGATGTCGTCACACGCGATGATGCCGATCCACTGGAT
AGCCGCGAGGCGTTGGAGCAACGTGAGATGCCGGACGAGCTGCCGCTGGATGCCAGCTGGGACGAGATCTATACCGCTGG
CACCCCCTCAGGCACGGGAACGGATTATCATGACGACGAGTTACCCGTGTTTCAGGGAGAGACGACCCAAACGCTGCAGG
ACTATCTGATGTGGCAGGTCGAACTGACCCCCTTCAGTGATACCGATCTGGCCATCGCGACCGCCATCGTCGATGCCGTC
GACGATACCGGCTATCTCAGCGCCTCGTTGGCGGAGATCCTCGAGAGTCTAGGGGACGATCGCGTCCTACCGGAAGAGAT
CGAGGCCGTCCTCAAGCGCATCCAACGTTTTGATCCGGTCGGTGTCGCCGCGCGTGATCTGCGCGACTGCCTACTGGTCC
AGCTCTCGCAATTCGCCGACGACACGCCCTATCTCAACGAGGCTCGTAGCATCGTCGATCGCCACCTCGATCTGTTGGGT
AATCACGATTTCCGAAATCTGATGCGTTCGACTAAGCTAAAAGAAGAGGTGCTCAAGGCGGCGATGCAACTGATCCAGAG
CCTCGACCCGCGGCCAGGACTCTCCATCAATACCGGCGAGTCTGACTATGTCATCCCCGACGTGCTGGTGCATAAACATC
AGGAACGCTGGGTGGTTGAGCTCAATCAGGACAGCGTCCCGCGCCTGCGTATCAATCAACATTACGCCGCGCTGGGCACT
CAGGCGCGTAACGATAGCGACAGTCAGTTCATTCGCAGCAATCTGCAAGAAGCGAAGTGGCTGATCAAGAGCCTAGAGAG
CCGTAACGATACCCTGCTGAAGGTATCGCGTTGCATCGTAGAGCAGCAGCAAGCGTTCTTCGAGCACGGGCCGGAGGCGA
TGAAGCCGATGGTACTGGCTGATATCGCTCAGCAAGTCGAAATGCACGAATCGACGATCTCACGTGTGACTACGCAGAAG
TTTCTGCACAGCCCGCGTGGAATCTTTGAACTGAAATATTTCTTCTCCAGCCATGTCAGCACCGAGGACGGCGGCGAGGC
CTCCTCCACGGCCATCCGGGCGCTGGTGAAGAAACTGATCGCGGCAGAAAATCCGGCGAAACCGCTGAGTGATAGCAAAC
TTACCACTCTATTGGCGGATCAGGGTATCATGGTCGCCCGGCGTACTGTCGCAAAATACCGAGAGTCTTTGTCCATTCCA
CCGTCCAATCAGCGTAAACAGCTGGTGTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(128-315)

Sigma-54, DNA binding domain (Sigma54_DBD)

(329-487)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.367

100

0.481