Detailed information    

insolico Bioinformatically predicted

Overview

Name   recQ   Type   Machinery gene
Locus tag   PHA55_RS00640 Genome accession   NZ_CP116044
Coordinates   127400..129229 (+) Length   609 a.a.
NCBI ID   WP_000035581.1    Uniprot ID   Q3YVE4
Organism   Escherichia coli strain STEC1575     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 122400..134229
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PHA55_RS00610 (PHA55_00610) - 122677..123525 (-) 849 WP_001059703.1 YPO3983 family protein -
  PHA55_RS00615 (PHA55_00615) - 123711..123857 (+) 147 Protein_112 CorA family divalent cation transporter -
  PHA55_RS00620 (PHA55_00620) - 124481..124780 (+) 300 WP_000526434.1 hypothetical protein -
  PHA55_RS00625 (PHA55_00625) rarD 124827..125714 (-) 888 WP_260589864.1 EamA family transporter RarD -
  PHA55_RS00630 (PHA55_00630) yigI 125766..126233 (-) 468 WP_001277142.1 acyl-CoA thioesterase YigI -
  PHA55_RS00635 (PHA55_00635) pldA 126398..127267 (+) 870 WP_001259700.1 phospholipase A -
  PHA55_RS00640 (PHA55_00640) recQ 127400..129229 (+) 1830 WP_000035581.1 ATP-dependent DNA helicase RecQ Machinery gene
  PHA55_RS00645 (PHA55_00645) rhtC 129293..129913 (+) 621 WP_000928824.1 threonine export protein RhtC -
  PHA55_RS00650 (PHA55_00650) rhtB 129975..130595 (-) 621 WP_000171710.1 homoserine/homoserine lactone efflux protein -
  PHA55_RS00655 (PHA55_00655) pldB 130706..131728 (+) 1023 WP_000487654.1 lysophospholipase L2 -
  PHA55_RS00660 (PHA55_00660) yigL 131736..132536 (+) 801 WP_000285352.1 sugar/pyridoxal phosphate phosphatase YigL -
  PHA55_RS00665 (PHA55_00665) bioP 132612..133511 (+) 900 WP_001196238.1 biotin transporter -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 68363.51 Da        Isoelectric Point: 7.2544

>NTDB_id=707074 PHA55_RS00640 WP_000035581.1 127400..129229(+) (recQ) [Escherichia coli strain STEC1575]
MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCLVVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKD
QVDQLQANGVAAACLNSTQTREQQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLMEKFKPLDQLMRYVQEQRGKSGI
IYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRADVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESY
YQETGRAGRDGLPAEAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEGRQEPCGNC
DICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDYGHDKLKVYGMGRDKSHEHWVSVIRQLIHLG
LVTQNIAQHSALQLTEAARPVLRGESSLQLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFND
ATLIEMAEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=707074 PHA55_RS00640 WP_000035581.1 127400..129229(+) (recQ) [Escherichia coli strain STEC1575]
GTGGCGCAGGCGGAAGTGTTGAATCTGGAGTCCGGAGCTAAACAGGTTTTACAAGAAACCTTTGGCTACCAACAGTTTCG
CCCCGGCCAGGAAGAAATTATCGACACTGTGCTTTCCGGTCGCGATTGCCTGGTCGTCATGCCCACCGGTGGCGGAAAAT
CCCTTTGCTATCAAATTCCTGCCTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGAT
CAGGTGGATCAACTGCAAGCCAACGGCGTGGCGGCGGCGTGCCTTAACTCGACGCAAACCCGCGAACAGCAACTTGAAGT
GATGACAGGCTGCCGCACCGGGCAAATTCGTCTGCTTTATATCGCCCCGGAACGCCTGATGCTGGATAACTTTCTTGAGC
ATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCG
GAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACAC
CACGCGCCAGGATATCGTGCGCCTGCTGGGGCTGAACGATCCGCTGATTCAAATCAGCAGTTTTGACCGTCCGAATATTC
GCTACATGCTGATGGAGAAGTTCAAACCGCTCGATCAGTTGATGCGCTACGTGCAGGAACAGCGCGGTAAGTCCGGCATT
ATCTACTGCAACAGCCGGGCGAAAGTAGAAGACACCGCTGCGCGCCTGCAAAGCAAGGGTATTAGCGCGGCGGCCTATCA
TGCCGGGCTGGAAAATAATGTCCGCGCCGACGTGCAGGAGAAATTCCAGCGCGATGACCTGCAAATTGTGGTGGCGACGG
TGGCGTTCGGCATGGGCATCAATAAACCAAACGTTCGCTTCGTGGTCCACTTTGATATTCCGCGCAATATCGAATCCTAT
TATCAGGAAACCGGTCGCGCCGGGCGTGATGGCCTGCCTGCGGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTG
GCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGT
TTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGC
GATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAA
TCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACA
AACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGC
CTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTC
TTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCA
AACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATTGCCGATGAAAGCAATGTCCCGCCGTACGTGGTGTTTAACGAC
GCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAA
GCTGGAACGCTTTGGTAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAG

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(28-187)

RecQ zinc-binding (RecQ_Zn_bind)

(343-404)

Helicase conserved C-terminal domain (Helicase_C)

(223-330)

HRDC domain (HRDC)

(533-599)

RQC domain (RQC)

(406-514)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YVE4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recQ Bacillus subtilis subsp. subtilis str. 168

40.203

97.209

0.391