Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   PGH49_RS02665 Genome accession   NZ_CP116035
Coordinates   558388..559821 (-) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli strain FAH     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 553388..564821
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PGH49_RS02630 (PGH49_02630) elbB 554079..554732 (+) 654 WP_000719791.1 isoprenoid biosynthesis glyoxalase ElbB -
  PGH49_RS02635 (PGH49_02635) mtgA 554729..555457 (+) 729 WP_000047069.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  PGH49_RS02640 (PGH49_02640) yrbL 555454..556086 (-) 633 WP_001539838.1 PhoP regulatory network protein YrbL -
  PGH49_RS02645 (PGH49_02645) npr 556300..556572 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  PGH49_RS02650 (PGH49_02650) rapZ 556569..557423 (-) 855 WP_000243741.1 RNase adapter RapZ -
  PGH49_RS02655 (PGH49_02655) ptsN 557469..557960 (-) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  PGH49_RS02660 (PGH49_02660) hpf 558078..558365 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  PGH49_RS02665 (PGH49_02665) rpoN/rpoN1 558388..559821 (-) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  PGH49_RS02670 (PGH49_02670) lptB 559869..560594 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  PGH49_RS02675 (PGH49_02675) lptA 560601..561158 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  PGH49_RS02680 (PGH49_02680) lptC 561127..561702 (-) 576 WP_001539833.1 LPS export ABC transporter periplasmic protein LptC -
  PGH49_RS02685 (PGH49_02685) kdsC 561699..562265 (-) 567 WP_000030015.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  PGH49_RS02690 (PGH49_02690) kdsD 562286..563272 (-) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  PGH49_RS02695 (PGH49_02695) yrbG 563286..564263 (-) 978 WP_000922880.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=706992 PGH49_RS02665 WP_000809051.1 558388..559821(-) (rpoN/rpoN1) [Escherichia coli strain FAH]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=706992 PGH49_RS02665 WP_000809051.1 558388..559821(-) (rpoN/rpoN1) [Escherichia coli strain FAH]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGATACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACACTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTATCAGGGCGAAACGACACAGACCTTACAGGATTACCTGATGTGGCAGGTCGAGTTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATTGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAAAGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465