Detailed information    

insolico Bioinformatically predicted

Overview

Name   stkP   Type   Regulator
Locus tag   PF977_RS13995 Genome accession   NZ_CP116012
Coordinates   2658992..2660938 (-) Length   648 a.a.
NCBI ID   WP_003232062.1    Uniprot ID   O34507
Organism   Bacillus subtilis strain SRCM124333     
Function   require for competence development; phosphorylate ComE (predicted from homology)   
Competence regulation

Genomic Context

Location: 2653992..2665938
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PF977_RS13965 (PF977_13965) yloU 2655654..2656016 (-) 363 WP_003232054.1 Asp23/Gls24 family envelope stress response protein -
  PF977_RS13970 (PF977_13970) rpmB 2656293..2656481 (+) 189 WP_003221548.1 50S ribosomal protein L28 -
  PF977_RS13975 (PF977_13975) spoVM 2656554..2656634 (-) 81 WP_003221545.1 stage V sporulation protein SpoVM -
  PF977_RS13980 (PF977_13980) thiN 2656706..2657350 (-) 645 WP_014479765.1 thiamine diphosphokinase -
  PF977_RS13985 (PF977_13985) rpe 2657423..2658076 (-) 654 WP_003232058.1 ribulose-phosphate 3-epimerase -
  PF977_RS13990 (PF977_13990) rsgA 2658081..2658977 (-) 897 WP_015715871.1 ribosome small subunit-dependent GTPase A -
  PF977_RS13995 (PF977_13995) stkP 2658992..2660938 (-) 1947 WP_003232062.1 serine/threonine protein kinase PrkC Regulator
  PF977_RS14000 (PF977_14000) prpC 2660932..2661696 (-) 765 WP_014479762.1 protein-serine/threonine phosphatase PrpC -
  PF977_RS14005 (PF977_14005) rlmN 2661703..2662794 (-) 1092 WP_015252110.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  PF977_RS14010 (PF977_14010) rsmB 2662798..2664141 (-) 1344 WP_015715870.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  PF977_RS14015 (PF977_14015) fmt 2664128..2665081 (-) 954 WP_015715869.1 methionyl-tRNA formyltransferase -
  PF977_RS14020 (PF977_14020) def 2665086..2665568 (-) 483 WP_015383630.1 peptide deformylase -

Sequence

Protein


Download         Length: 648 a.a.        Molecular weight: 71866.49 Da        Isoelectric Point: 4.7568

>NTDB_id=706914 PF977_RS13995 WP_003232062.1 2658992..2660938(-) (stkP) [Bacillus subtilis strain SRCM124333]
MLIGKRISGRYQILRVIGGGGMANVYLAEDIILDREVAIKILRFDYANDNEFIRRFRREAQSASSLDHPNIVSIYDLGEE
DDIYYIVMEYVEGMTLKEYITANGPLHPKEALNIMEQIVSAIAHAHQNQIVHRDIKPHNILIDHMGNIKVTDFGIATALS
STTITHTNSVLGSVHYLSPEQARGGLATKKSDIYALGIVLFELLTGRIPFDGESAVSIALKHLQAETPSAKRWNPSVPQS
VENIILKATAKDPFHRYETAEDMEADIKTAFDADRLNEKRFTIQEDEEMTKAIPIIKDEELAKAAGEKEAEVTTAQENKT
KKNGKRKKWPWVLLTICLVFITAGILAVTVFPSLFMPKDVKIPDVSGMEYEKAAGLLEKEGLQVDSEVLEISDEKIEEGL
MVKTDPKADTTVKEGATVTLYKSTGKAKTEIGDVTGQTVDQAKKALKDQGFNHVTVNEVNDEKNAGTVIDQNPSAGTELV
PSEDQVKLTVSIGPEDITLRDLKTYSKEAASGYLEDNGLKLVEKEAYSDDVPEGQVVKQKPAAGTAVKPGNEVEVTFSLG
PEKKPAKTVKEKVKIPYEPENEGDELQVQIAVDDADHSISDTYEEFKIKEPTERTIELKIEPGQKGYYQVMVNNKVVSYK
TIEYPKDE

Nucleotide


Download         Length: 1947 bp        

>NTDB_id=706914 PF977_RS13995 WP_003232062.1 2658992..2660938(-) (stkP) [Bacillus subtilis strain SRCM124333]
GTGCTAATCGGCAAGCGGATCAGCGGGCGTTACCAAATTCTCCGCGTTATAGGCGGCGGGGGAATGGCCAACGTGTATTT
AGCTGAGGATATCATTCTAGACCGTGAAGTCGCAATTAAAATCCTGCGGTTTGACTATGCAAATGACAATGAATTTATCA
GGCGTTTCCGCAGAGAAGCCCAATCCGCATCAAGCCTCGATCACCCGAATATTGTCAGCATTTACGATTTGGGCGAGGAA
GATGATATTTATTATATTGTCATGGAATACGTTGAAGGCATGACGCTTAAAGAATACATAACAGCAAATGGGCCGCTTCA
CCCTAAAGAAGCGCTGAACATCATGGAGCAAATTGTCTCAGCCATCGCTCATGCCCATCAAAACCAGATTGTTCACAGAG
ACATCAAGCCGCATAACATTTTGATTGATCATATGGGAAATATCAAAGTAACGGATTTTGGAATTGCGACGGCACTAAGT
TCGACCACAATCACCCATACCAATTCAGTTCTGGGCTCGGTCCATTACTTATCACCTGAACAGGCCCGGGGCGGCTTAGC
CACAAAAAAATCGGATATTTATGCGCTTGGAATCGTTCTATTTGAGCTTTTAACCGGCCGTATTCCGTTTGATGGAGAGT
CAGCAGTCAGCATCGCCTTGAAGCATCTTCAAGCGGAAACTCCTTCGGCAAAAAGGTGGAATCCATCGGTCCCCCAAAGC
GTTGAAAACATCATACTCAAGGCAACTGCCAAAGATCCTTTTCATCGCTACGAAACGGCTGAAGACATGGAAGCAGACAT
AAAAACAGCTTTTGATGCCGACAGACTCAATGAAAAGAGATTTACGATTCAAGAAGATGAAGAAATGACAAAAGCGATAC
CTATCATTAAAGATGAAGAACTCGCTAAAGCTGCTGGCGAAAAAGAAGCTGAAGTGACAACCGCACAAGAAAACAAAACA
AAGAAGAACGGCAAAAGAAAAAAGTGGCCGTGGGTTTTGCTCACGATATGCCTCGTTTTTATCACAGCTGGAATTCTTGC
TGTCACTGTTTTTCCGTCGCTTTTCATGCCTAAGGATGTCAAAATACCTGATGTCTCCGGAATGGAATACGAAAAAGCCG
CAGGGCTCTTGGAAAAAGAAGGTTTACAGGTTGATTCCGAGGTGTTGGAAATCTCAGATGAAAAAATTGAAGAGGGCCTG
ATGGTAAAAACGGATCCTAAAGCGGATACCACAGTCAAAGAAGGCGCCACGGTCACCCTTTATAAGAGCACCGGAAAAGC
AAAAACGGAGATCGGTGATGTGACAGGCCAAACGGTCGACCAAGCAAAAAAAGCGTTGAAGGACCAAGGGTTTAATCATG
TAACAGTAAATGAAGTGAATGACGAGAAAAATGCGGGCACTGTCATTGACCAAAATCCTTCAGCAGGGACTGAGCTGGTC
CCGAGTGAAGATCAAGTCAAACTTACAGTCAGTATCGGACCCGAAGACATTACGCTTAGAGACTTGAAAACCTACAGTAA
AGAAGCAGCGTCTGGATATCTGGAAGACAACGGATTGAAGCTTGTAGAAAAAGAAGCATACTCAGATGATGTTCCAGAAG
GACAGGTTGTCAAACAAAAACCAGCAGCAGGTACGGCAGTAAAGCCGGGAAACGAAGTTGAAGTGACATTCTCTCTCGGA
CCAGAGAAAAAACCTGCGAAAACAGTGAAAGAAAAGGTCAAGATCCCCTACGAACCAGAAAATGAAGGGGACGAGCTTCA
AGTGCAAATCGCGGTTGACGATGCGGATCACAGCATCTCTGACACTTACGAAGAATTTAAGATAAAAGAGCCGACTGAAC
GAACGATCGAACTAAAGATTGAACCCGGCCAAAAAGGGTACTATCAAGTAATGGTAAACAATAAAGTTGTCAGCTACAAA
ACCATTGAGTATCCAAAAGATGAATAA

Domains

Predicted by InterProScan.

Protein kinase domain (Pkinase)

(11-261)

PASTA domain (PASTA)

(360-423)

PASTA domain (PASTA)

(430-491)

PASTA domain (PASTA)

(498-558)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB O34507

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  stkP Streptococcus pneumoniae TIGR4

40.555

89.043

0.361