Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   NMD93_RS14130 Genome accession   NZ_CP100775
Coordinates   3030208..3031677 (+) Length   489 a.a.
NCBI ID   WP_005281701.1    Uniprot ID   D4F169
Organism   Edwardsiella tarda strain GT151     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3025208..3036677
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD93_RS14100 (NMD93_14075) - 3025638..3026627 (+) 990 WP_005281708.1 calcium/sodium antiporter -
  NMD93_RS14105 (NMD93_14080) kdsD 3026680..3027666 (+) 987 WP_047060479.1 arabinose-5-phosphate isomerase KdsD -
  NMD93_RS14110 (NMD93_14085) kdsC 3027682..3028248 (+) 567 WP_005297337.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  NMD93_RS14115 (NMD93_14090) lptC 3028245..3028817 (+) 573 WP_005281705.1 LPS export ABC transporter periplasmic protein LptC -
  NMD93_RS14120 (NMD93_14095) lptA 3028792..3029349 (+) 558 WP_005281704.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  NMD93_RS14125 (NMD93_14100) lptB 3029356..3030081 (+) 726 WP_005281703.1 LPS export ABC transporter ATP-binding protein -
  NMD93_RS14130 (NMD93_14105) rpoN/rpoN1 3030208..3031677 (+) 1470 WP_005281701.1 RNA polymerase factor sigma-54 Machinery gene
  NMD93_RS14135 (NMD93_14110) hpf 3031699..3031986 (+) 288 WP_024524583.1 ribosome hibernation promoting factor -
  NMD93_RS14140 (NMD93_14115) ptsN 3032122..3032568 (+) 447 WP_005297331.1 PTS IIA-like nitrogen regulatory protein PtsN -
  NMD93_RS14145 (NMD93_14120) rapZ 3032676..3033527 (+) 852 WP_005281696.1 RNase adapter RapZ -
  NMD93_RS14150 (NMD93_14125) npr 3033535..3033807 (+) 273 WP_370554618.1 PTS phosphocarrier protein NPr -
  NMD93_RS14155 (NMD93_14130) rnk 3033984..3034394 (+) 411 WP_068871249.1 nucleoside diphosphate kinase regulator -
  NMD93_RS14160 (NMD93_14135) - 3034425..3034940 (-) 516 WP_370554619.1 cytochrome b/b6 domain-containing protein -
  NMD93_RS14165 (NMD93_14140) cybC 3034946..3035332 (-) 387 WP_005291336.1 cytochrome b562 -
  NMD93_RS14170 (NMD93_14145) deoD 3035546..3036271 (-) 726 WP_005281692.1 purine-nucleoside phosphorylase -

Sequence

Protein


Download         Length: 489 a.a.        Molecular weight: 55075.91 Da        Isoelectric Point: 4.4068

>NTDB_id=706862 NMD93_RS14130 WP_005281701.1 3030208..3031677(+) (rpoN/rpoN1) [Edwardsiella tarda strain GT151]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQEIQQALESNPLLEQLDPNEEAGDDGVVRENTQEDVVTRDDADPLD
SREALEQREMPDELPLDASWDEIYTAGTPSGTGTDYHDDELPVFQGETTQTLQDYLMWQVELTPFSDTDLAIATAIVDAV
DDTGYLSASLEEILESLGDDRVLPEEIEAVLKRIQRFDPVGVAARDLRDCLLVQLSQFADDTPYLNEARSIVDRHLDLLG
NHDFRNLMRSTKLKEEVLKAAMQLIQSLDPRPGLSINTGESDYVIPDVLVHKHQERWVVELNQDSVPRLRINQHYAALGT
QARNDSDSQFIRSNLQEAKWLIKSLESRNDTLLKVSRCIVEQQQAFFEHGPEAMKPMVLADIAQQVEMHESTISRVTTQK
FLHSPRGIFELKYFFSSHVSTEDGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTLLADQGIMVARRTVAKYRESLSIP
PSNQRKQLV

Nucleotide


Download         Length: 1470 bp        

>NTDB_id=706862 NMD93_RS14130 WP_005281701.1 3030208..3031677(+) (rpoN/rpoN1) [Edwardsiella tarda strain GT151]
ATGAAGCAAGGCTTGCAACTCAGACTCAGCCAGCAGCTGGCCATGACGCCCCAACTGCAACAGGCCATTCGCCTGTTGCA
GCTCTCGACGCTGGAGTTACAGCAAGAGATCCAGCAGGCGTTGGAAAGCAATCCTCTGCTGGAACAACTGGATCCCAATG
AGGAAGCCGGCGACGACGGCGTCGTTCGCGAGAATACACAGGAGGATGTCGTCACACGCGATGATGCCGATCCACTGGAT
AGCCGCGAGGCGTTGGAGCAACGTGAGATGCCGGACGAGCTGCCGCTGGATGCCAGCTGGGACGAGATCTATACCGCTGG
CACCCCCTCAGGCACGGGAACGGATTATCATGACGACGAGTTACCCGTGTTTCAGGGAGAGACGACCCAAACGCTGCAGG
ACTATCTGATGTGGCAGGTCGAACTGACCCCCTTCAGTGATACCGATCTGGCCATCGCGACCGCCATCGTCGATGCCGTC
GACGATACCGGCTATCTCAGCGCTTCGTTGGAGGAGATCCTCGAGAGTCTAGGGGACGATCGCGTCCTACCGGAAGAGAT
CGAGGCCGTCCTCAAGCGCATCCAACGTTTTGATCCGGTCGGTGTCGCCGCGCGTGATCTGCGCGACTGCCTACTGGTCC
AGCTCTCGCAATTCGCCGACGACACGCCCTATCTCAACGAGGCTCGTAGCATCGTCGATCGCCACCTCGATCTGTTGGGT
AATCACGATTTCCGAAATCTGATGCGTTCGACTAAGCTAAAAGAAGAGGTGCTCAAGGCGGCGATGCAACTGATCCAGAG
CCTCGACCCGCGGCCAGGACTCTCCATCAACACCGGCGAGTCTGACTACGTCATCCCCGACGTGCTGGTGCATAAACATC
AGGAGCGCTGGGTGGTTGAGCTCAATCAGGACAGCGTCCCGCGCCTGCGTATCAACCAACATTACGCCGCGCTGGGCACT
CAGGCGCGTAACGACAGCGACAGCCAGTTCATTCGCAGCAATCTGCAAGAAGCGAAGTGGCTGATCAAGAGCCTAGAGAG
CCGTAACGATACCCTGCTGAAGGTATCGCGTTGCATCGTAGAGCAGCAGCAAGCGTTCTTCGAGCACGGGCCGGAGGCGA
TGAAGCCGATGGTACTGGCTGATATCGCTCAGCAAGTCGAAATGCACGAATCGACGATCTCACGTGTGACTACGCAGAAG
TTTCTGCACAGCCCGCGTGGAATCTTTGAACTGAAATATTTCTTCTCCAGCCATGTCAGCACCGAGGACGGCGGCGAGGC
CTCCTCCACGGCCATCCGGGCGCTGGTGAAGAAACTGATCGCGGCAGAAAATCCGGCGAAACCGCTGAGTGATAGCAAAC
TTACCACTCTATTGGCGGATCAGGGTATCATGGTCGCCCGGCGTACTGTCGCAAAATACCGAGAGTCTTTGTCCATTCCA
CCGTCCAATCAGCGTAAACAGCTGGTGTGA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(329-487)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(128-315)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D4F169

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.367

100

0.481