Detailed information    

insolico Bioinformatically predicted

Overview

Name   helD   Type   Machinery gene
Locus tag   PF977_RS03930 Genome accession   NZ_CP116012
Coordinates   772549..774873 (+) Length   774 a.a.
NCBI ID   WP_015714702.1    Uniprot ID   -
Organism   Bacillus subtilis strain SRCM124333     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 767549..779873
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PF977_RS03910 (PF977_03910) cadA 768239..770347 (+) 2109 WP_133516494.1 heavy metal translocating P-type ATPase -
  PF977_RS03915 (PF977_03915) bdbD 770467..771135 (+) 669 WP_015714703.1 disulfide bond formation protein BdbD -
  PF977_RS03920 (PF977_03920) bdbC 771140..771556 (+) 417 WP_015384637.1 disulfide oxidoreductase -
  PF977_RS03925 (PF977_03925) yvgT 771743..772351 (+) 609 WP_003219999.1 trimeric intracellular cation channel family protein -
  PF977_RS03930 (PF977_03930) helD 772549..774873 (+) 2325 WP_015714702.1 DNA helicase IV Machinery gene
  PF977_RS03935 (PF977_03935) cysJ 775044..776861 (+) 1818 WP_015714701.1 assimilatory sulfite reductase (NADPH) flavoprotein subunit -
  PF977_RS03940 (PF977_03940) cysI 776887..778602 (+) 1716 WP_015714700.1 assimilatory sulfite reductase (NADPH) hemoprotein subunit -

Sequence

Protein


Download         Length: 774 a.a.        Molecular weight: 89894.58 Da        Isoelectric Point: 6.5798

>NTDB_id=706852 PF977_RS03930 WP_015714702.1 772549..774873(+) (helD) [Bacillus subtilis strain SRCM124333]
MNQQDKEWKEEQSRIDEVLKELEKKERFLETSAGGLKHDIIGLRKSFWEDVKVNFDDAHEAIETMASIKQQAELLSDREQ
NHRRMDQQLKRIHRLKKSPYFGRIDFIENGEAEAERIYIGLASCLDEKEEHFLIYDWRAPISSLYYNYSPGKAEYEVPGE
TIEGEMVLKRQFMIKNGDLKAMFNTDMTIGDEMLQEVLSHHSDTQMKNIVSTIQKEQNQIIRNEKSKVLIVQGAAGSGKT
SAALQRVAYLLYRHRGVIDAGQIVLFSPNFLFNSYVSSVLPELGEENMEQATFQEYIEHRLGRKFKCESPFDQLEYCLTE
TKGGDFPTRLAGITWKAGLSFQQFINEYVTRLSSEGMIFKNIIFRGQKLITKEQIQSYFYSLDQNHSIPNRMEQTAKWLL
SELNKLEKKERRKDWVVHEAELLDKEDYLDVYKKLQERKRFSESTFNDYQREQQLLAAIIVKKAFKPLKQAVRLLAFLDV
SQLYLQLFSGWGGKFQHEKMAVIGELTRSAFTDNKLLYEDAAPFLYMQDLIEGRKKNTKIKHLFIDEAQDYSPFQMAYMR
SIFPAASMTVLGDINQSIYAHTIHGDQRMDACFEDDPAEYVRLKRTYRSTRQIVEFTKAMLQDGADIEPFNRSGEMPLVV
KTEGHESLCQKLAQEIGRLKKKGRETIAVICKTAQQCIQAHAHMSEYTDVRLIHKENQPFQKGVCVIPVYLAKGIEFDAV
LVYDASEEHYHTEHDRRLLYTACTRAMHMLAVFYTGEASPFVTAVPPHLYQMAE

Nucleotide


Download         Length: 2325 bp        

>NTDB_id=706852 PF977_RS03930 WP_015714702.1 772549..774873(+) (helD) [Bacillus subtilis strain SRCM124333]
ATGAATCAGCAGGATAAGGAATGGAAGGAAGAGCAGTCGAGAATAGATGAGGTGCTGAAGGAGCTCGAAAAAAAAGAGCG
GTTTCTGGAAACGTCTGCGGGCGGGTTGAAGCATGATATCATCGGATTAAGAAAAAGCTTTTGGGAGGATGTTAAGGTTA
ATTTTGATGATGCCCATGAAGCGATTGAAACGATGGCAAGCATTAAGCAGCAGGCAGAGCTGCTGTCTGATCGGGAACAG
AATCATCGGCGCATGGATCAGCAGCTCAAACGAATTCATCGATTGAAAAAATCGCCTTATTTCGGCCGGATTGATTTTAT
TGAAAACGGCGAGGCGGAGGCAGAGCGAATTTATATCGGTTTAGCTTCCTGTTTGGATGAGAAGGAGGAACACTTTCTAA
TCTATGACTGGAGGGCGCCGATATCAAGTTTGTACTACAATTATTCCCCAGGAAAAGCGGAGTACGAGGTGCCCGGTGAA
ACGATAGAAGGGGAAATGGTGCTGAAACGCCAGTTTATGATCAAAAACGGCGATCTCAAGGCGATGTTTAATACCGATAT
GACGATTGGTGATGAAATGCTGCAAGAGGTATTAAGCCATCACTCCGATACCCAAATGAAAAACATCGTGTCTACGATTC
AGAAAGAACAAAATCAGATTATCCGTAATGAAAAAAGCAAGGTCCTGATCGTGCAGGGAGCGGCTGGCAGCGGGAAAACA
TCGGCTGCGCTTCAGCGTGTCGCTTATTTGTTATACAGGCACCGCGGTGTCATTGATGCCGGACAAATCGTTTTGTTTTC
TCCTAACTTCTTATTTAACAGCTATGTCTCCTCTGTTTTGCCGGAGTTAGGAGAAGAGAATATGGAGCAGGCGACGTTTC
AGGAATATATCGAGCATCGTCTTGGCCGCAAGTTCAAATGTGAAAGCCCCTTTGATCAGCTCGAATATTGCCTGACCGAA
ACAAAGGGAGGCGATTTTCCAACAAGGCTTGCGGGAATCACATGGAAGGCGGGTTTATCCTTTCAGCAATTCATTAATGA
ATACGTCACACGGCTTTCCTCTGAAGGGATGATTTTTAAAAATATTATATTCCGCGGACAAAAGCTGATCACAAAGGAGC
AAATTCAATCCTATTTTTACTCATTGGATCAAAATCACTCCATTCCGAATCGGATGGAGCAAACCGCGAAGTGGCTGCTG
AGTGAGCTTAACAAGCTTGAGAAAAAGGAACGGCGAAAGGATTGGGTTGTTCATGAAGCTGAGCTGCTTGACAAAGAGGA
CTACCTGGACGTGTACAAAAAACTGCAGGAAAGGAAACGGTTCAGCGAGAGTACGTTTAACGATTATCAAAGAGAGCAGC
AGCTGCTTGCCGCCATCATTGTGAAAAAAGCTTTCAAACCGCTCAAGCAGGCAGTCAGGCTCCTTGCATTTCTTGATGTT
TCACAGCTGTATTTGCAATTGTTTTCTGGCTGGGGAGGGAAATTTCAGCATGAGAAGATGGCCGTGATAGGTGAGCTGAC
CCGTTCGGCATTTACGGATAATAAATTGCTCTATGAGGACGCGGCACCGTTTCTTTATATGCAGGATCTGATTGAGGGCA
GAAAGAAAAATACGAAGATCAAACATCTATTTATTGACGAAGCCCAGGACTATTCACCGTTTCAGATGGCTTACATGAGA
AGTATTTTTCCGGCTGCCAGCATGACAGTGCTCGGTGATATCAATCAGTCCATCTACGCGCATACCATACACGGGGATCA
GCGTATGGATGCGTGCTTTGAGGATGATCCTGCTGAGTATGTTCGTTTAAAGAGAACATACAGATCAACAAGGCAGATTG
TCGAGTTTACCAAAGCGATGCTTCAAGATGGAGCGGATATTGAGCCGTTTAATAGAAGCGGGGAAATGCCGCTCGTTGTC
AAAACTGAAGGACATGAGAGCCTTTGCCAAAAGCTCGCGCAAGAAATTGGCCGGCTCAAGAAAAAAGGGCGCGAAACAAT
AGCCGTCATCTGCAAGACTGCGCAGCAATGCATACAGGCGCACGCCCACATGAGTGAATATACAGATGTTCGCCTGATTC
ATAAAGAAAATCAACCATTTCAAAAGGGTGTTTGCGTGATTCCGGTGTATTTAGCGAAGGGAATTGAATTTGACGCAGTG
CTTGTCTATGATGCATCTGAAGAACACTATCATACAGAGCACGACCGCAGGCTTTTATATACTGCGTGCACCCGTGCGAT
GCACATGCTGGCAGTATTTTATACAGGTGAAGCCAGTCCGTTTGTAACCGCTGTGCCGCCTCACTTATATCAGATGGCTG
AATGA

Domains

Predicted by InterProScan.

UvrD-like helicase C-terminal domain (UvrD_C_2)

(704-751)

AAA domain (AAA_19)

(217-580)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  helD Bacillus subtilis subsp. subtilis str. 168

98.191

100

0.982