Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   NMD96_RS14800 Genome accession   NZ_CP100768
Coordinates   3168588..3170057 (+) Length   489 a.a.
NCBI ID   WP_370555935.1    Uniprot ID   -
Organism   Edwardsiella tarda strain GT168     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3163588..3175057
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD96_RS14770 (NMD96_14730) - 3164018..3165007 (+) 990 WP_078000403.1 calcium/sodium antiporter -
  NMD96_RS14775 (NMD96_14735) kdsD 3165060..3166046 (+) 987 WP_078000404.1 arabinose-5-phosphate isomerase KdsD -
  NMD96_RS14780 (NMD96_14740) kdsC 3166062..3166628 (+) 567 WP_370555934.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  NMD96_RS14785 (NMD96_14745) lptC 3166625..3167197 (+) 573 WP_035604656.1 LPS export ABC transporter periplasmic protein LptC -
  NMD96_RS14790 (NMD96_14750) lptA 3167172..3167729 (+) 558 WP_005281704.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  NMD96_RS14795 (NMD96_14755) lptB 3167736..3168461 (+) 726 WP_005281703.1 LPS export ABC transporter ATP-binding protein -
  NMD96_RS14800 (NMD96_14760) rpoN/rpoN1 3168588..3170057 (+) 1470 WP_370555935.1 RNA polymerase factor sigma-54 Machinery gene
  NMD96_RS14805 (NMD96_14765) hpf 3170079..3170366 (+) 288 WP_024524583.1 ribosome hibernation promoting factor -
  NMD96_RS14810 (NMD96_14770) ptsN 3170501..3170947 (+) 447 WP_005297331.1 PTS IIA-like nitrogen regulatory protein PtsN -
  NMD96_RS14815 (NMD96_14775) rapZ 3171055..3171906 (+) 852 WP_005281696.1 RNase adapter RapZ -
  NMD96_RS14820 (NMD96_14780) npr 3171914..3172186 (+) 273 WP_005281695.1 PTS phosphocarrier protein NPr -
  NMD96_RS14825 (NMD96_14785) rnk 3172363..3172773 (+) 411 WP_370555936.1 nucleoside diphosphate kinase regulator -
  NMD96_RS14830 (NMD96_14790) - 3172809..3173312 (-) 504 WP_035604652.1 cytochrome b/b6 domain-containing protein -
  NMD96_RS14835 (NMD96_14795) cybC 3173318..3173704 (-) 387 WP_035604649.1 cytochrome b562 -
  NMD96_RS14840 (NMD96_14800) tnpA 3173930..3174367 (-) 438 WP_035598237.1 IS200/IS605 family transposase -

Sequence

Protein


Download         Length: 489 a.a.        Molecular weight: 54987.85 Da        Isoelectric Point: 4.4249

>NTDB_id=706821 NMD96_RS14800 WP_370555935.1 3168588..3170057(+) (rpoN/rpoN1) [Edwardsiella tarda strain GT168]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQEIQQALESNPLLEQLDPNEEAGDDGVVRENTQEDVVTRDDADPLD
SREALEQREMPDELPLDASWDEIYTAGTPSGTGTDYHDDELPVFQGETTQTLQDYLMWQVELTPFSDTDLAIATAIVDAV
DDTGYLSASLAEILESLGDDRVLPEEIEAVLKRIQRFDPVGVAARDLRDCLLVQLSQFADDTPYLNEARSIVDRHLDLLG
NHDFRNLMRSTKLKEEVLKAAMQLIQSLDPRPGLSINTGESDYVIPDVLVHKHQERWVVELNQDSVPRLRINQHYAALGA
QARNDSDSQFIRSNLQEAKWLIKSLESRNDTLLKVSRCIVEQQQAFFEHGPEAMKPMVLADIAQQVEMHESTISRVTTQK
FLHSPRGIFELKYFFSSHVSTEDGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTLLADQGIMVARRTVAKYRESLSIP
PSNQRKQLV

Nucleotide


Download         Length: 1470 bp        

>NTDB_id=706821 NMD96_RS14800 WP_370555935.1 3168588..3170057(+) (rpoN/rpoN1) [Edwardsiella tarda strain GT168]
ATGAAGCAAGGCTTGCAACTCAGACTCAGCCAGCAGTTGGCCATGACGCCCCAACTGCAACAGGCCATTCGCCTGTTGCA
GCTCTCGACGCTGGAGTTACAGCAAGAGATCCAGCAGGCGTTGGAAAGCAATCCTCTGCTGGAACAACTGGATCCCAATG
AGGAAGCCGGCGACGACGGCGTCGTTCGCGAGAATACACAGGAGGATGTCGTCACACGCGATGATGCCGATCCACTGGAT
AGCCGCGAGGCGTTGGAGCAACGTGAGATGCCGGACGAGCTGCCGCTGGATGCCAGCTGGGACGAGATCTATACCGCTGG
CACCCCCTCAGGCACGGGAACGGATTATCATGACGACGAGTTACCCGTGTTTCAGGGAGAGACGACCCAAACGCTGCAGG
ACTATCTGATGTGGCAGGTCGAACTGACCCCCTTCAGTGATACCGATCTGGCCATCGCTACCGCCATCGTCGATGCCGTC
GACGATACCGGCTATCTCAGCGCTTCGTTGGCGGAGATCCTCGAGAGTCTGGGGGACGATCGCGTCCTACCGGAAGAGAT
CGAGGCCGTCCTCAAGCGCATCCAACGTTTTGATCCGGTCGGTGTCGCCGCGCGTGATCTGCGCGACTGCCTACTGGTCC
AGCTCTCGCAATTCGCCGACGACACGCCCTATCTCAACGAGGCTCGTAGCATCGTCGATCGCCACCTCGATCTGTTGGGT
AATCACGATTTCCGAAATCTGATGCGTTCGACTAAGCTAAAAGAAGAGGTGCTCAAGGCGGCGATGCAACTGATCCAGAG
CCTCGACCCGCGGCCAGGACTCTCCATCAATACCGGCGAGTCTGACTACGTCATCCCCGACGTGCTGGTGCATAAACATC
AGGAACGCTGGGTGGTTGAGCTCAATCAGGACAGCGTCCCGCGCCTGCGTATCAACCAACATTACGCCGCGCTGGGCGCT
CAGGCGCGTAACGACAGCGACAGCCAGTTCATTCGCAGCAATCTGCAAGAAGCGAAGTGGCTGATCAAGAGCCTAGAGAG
CCGTAACGATACCCTGCTGAAGGTATCGCGTTGCATCGTAGAGCAGCAGCAAGCGTTCTTCGAGCACGGGCCGGAGGCGA
TGAAGCCGATGGTACTGGCTGATATCGCTCAGCAAGTCGAAATGCACGAATCGACGATCTCACGTGTGACTACGCAGAAG
TTTCTGCACAGCCCGCGTGGAATCTTTGAACTGAAATATTTCTTCTCCAGCCATGTCAGCACCGAGGACGGCGGCGAGGC
CTCCTCCACGGCCATCCGGGCGCTGGTGAAGAAACTGATCGCGGCAGAAAATCCGGCGAAACCGCTGAGTGATAGCAAAC
TTACCACTCTATTGGCAGATCAGGGTATCATGGTCGCCCGGCGTACTGTCGCAAAATACCGAGAGTCTTTGTCCATTCCA
CCGTCCAATCAGCGTAAACAGCTGGTGTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(128-315)

Sigma-54, DNA binding domain (Sigma54_DBD)

(329-487)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.367

100

0.481