Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   PF976_RS16700 Genome accession   NZ_CP116011
Coordinates   3213183..3214493 (-) Length   436 a.a.
NCBI ID   WP_014471057.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain SRCM124317     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3208183..3219493
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PF976_RS16680 (PF976_16680) - 3208426..3209142 (-) 717 WP_013353675.1 (Fe-S)-binding protein -
  PF976_RS16685 (PF976_16685) - 3209324..3210514 (-) 1191 Protein_3252 PLP-dependent aminotransferase family protein -
  PF976_RS16690 (PF976_16690) - 3210545..3211264 (-) 720 WP_013353677.1 FadR/GntR family transcriptional regulator -
  PF976_RS16695 (PF976_16695) lutP 3211469..3213145 (+) 1677 WP_013353678.1 L-lactate permease LutP -
  PF976_RS16700 (PF976_16700) rpoN/rpoN1 3213183..3214493 (-) 1311 WP_014471057.1 RNA polymerase factor sigma-54 Machinery gene
  PF976_RS16705 (PF976_16705) yvfG 3214571..3214789 (+) 219 WP_003151570.1 protein YvfG -
  PF976_RS16710 (PF976_16710) - 3214803..3215765 (-) 963 WP_013353680.1 polysaccharide pyruvyl transferase family protein -
  PF976_RS16715 (PF976_16715) - 3215744..3216916 (-) 1173 WP_013353681.1 DegT/DnrJ/EryC1/StrS family aminotransferase -
  PF976_RS16720 (PF976_16720) - 3216921..3217568 (-) 648 WP_013353682.1 acetyltransferase -
  PF976_RS16725 (PF976_16725) - 3217565..3218173 (-) 609 WP_014471058.1 sugar transferase -

Sequence

Protein


Download         Length: 436 a.a.        Molecular weight: 49879.01 Da        Isoelectric Point: 9.3742

>NTDB_id=706805 PF976_RS16700 WP_014471057.1 3213183..3214493(-) (rpoN/rpoN1) [Bacillus amyloliquefaciens strain SRCM124317]
MDIKLQQTQILKPQLTQELKQAITLLGYNSAELAGYIDELSLENPLIERKETDTPPLSYHKTNKNRMKEQDVSQQIRLTR
KTLQDSLKQQAIDMKLSDAEKKIFNYLIYSLDSNGYLKEDIQAAADHVSASLEETEAVLAKLQSLEPAGIGARSLQECIL
LQLRRLPERNEQAEWIAGAYFELFARKNWKALTLQTGIPLKDIQDIFDMISALDPRPGLRYAHEEPGFYVEPDIFITVKN
GIIQARLNSRSFPDIQLHAHYRPMLSAAGCRDTKDYLSAKYQEWRWLNRALSQRKQTITRIIGVLTERQASFFLTGKSGM
QPLTLREVAETLELHESTISRAIKGKYVQTPYGLYDMKTFFSAKAESSGDGSASNYAVKAHLEALVRKEDKKKPFSDQKL
ADLLLEQYDIRISRRTVAKYRDQLNIPPSSARKRYS

Nucleotide


Download         Length: 1311 bp        

>NTDB_id=706805 PF976_RS16700 WP_014471057.1 3213183..3214493(-) (rpoN/rpoN1) [Bacillus amyloliquefaciens strain SRCM124317]
ATGGATATCAAACTTCAGCAAACACAAATTTTAAAGCCTCAATTGACTCAAGAGCTTAAACAGGCCATCACTTTACTCGG
ATACAATTCCGCGGAACTGGCCGGGTATATTGATGAGCTCTCTCTGGAAAACCCTCTGATTGAACGAAAGGAAACAGACA
CGCCGCCACTATCTTACCATAAAACAAATAAAAACAGGATGAAAGAACAGGACGTAAGCCAGCAGATCAGGCTTACGCGC
AAAACGCTGCAGGATTCGCTCAAACAGCAGGCCATAGACATGAAGCTGTCCGATGCTGAAAAGAAAATTTTTAATTATCT
GATATATTCCTTGGATTCTAACGGATATTTAAAAGAGGACATACAAGCTGCCGCCGACCATGTATCCGCTTCACTAGAGG
AGACGGAAGCCGTTCTGGCAAAGCTTCAATCGCTTGAGCCCGCGGGAATCGGCGCCAGGTCTTTGCAGGAATGCATCCTT
CTTCAGCTCCGCCGTCTGCCCGAGCGGAACGAACAGGCGGAATGGATTGCCGGCGCCTATTTTGAGCTTTTTGCAAGAAA
GAACTGGAAAGCGCTTACATTGCAAACGGGCATTCCGCTCAAAGACATTCAGGACATCTTTGACATGATCAGCGCGCTTG
ACCCGAGGCCGGGGCTCCGTTATGCGCATGAGGAACCGGGTTTTTACGTTGAACCGGATATTTTCATCACGGTGAAAAAC
GGCATCATTCAAGCGCGGCTCAACAGCCGTTCGTTCCCGGATATCCAGCTTCATGCCCATTATCGGCCGATGCTGTCAGC
CGCCGGATGCCGTGACACGAAAGATTATCTGTCGGCAAAATATCAGGAATGGCGCTGGCTGAACCGGGCTTTATCGCAAA
GAAAACAAACGATCACGAGAATTATCGGCGTCCTGACTGAACGGCAGGCATCCTTTTTTCTCACAGGAAAAAGCGGCATG
CAGCCGCTGACCTTAAGAGAGGTGGCGGAAACGCTGGAGCTCCATGAATCAACGATCAGCCGGGCCATCAAAGGAAAGTA
CGTGCAGACGCCGTACGGACTGTACGATATGAAAACATTTTTTTCGGCGAAAGCTGAATCATCGGGAGACGGCAGCGCCT
CAAACTATGCCGTAAAAGCACACCTGGAAGCATTGGTCCGTAAAGAAGACAAAAAGAAACCTTTTTCAGACCAAAAGCTT
GCCGACCTGCTGCTTGAACAATATGACATCCGAATTTCAAGACGCACCGTCGCCAAATACCGTGACCAATTAAATATCCC
GCCGTCATCGGCGAGAAAACGATACAGCTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(275-434)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(77-261)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

32.803

100

0.378