Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilH   Type   Machinery gene
Locus tag   PF976_RS08215 Genome accession   NZ_CP116011
Coordinates   1555465..1555827 (+) Length   120 a.a.
NCBI ID   WP_003154238.1    Uniprot ID   A0A150FA43
Organism   Bacillus amyloliquefaciens strain SRCM124317     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1550465..1560827
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PF976_RS08185 (PF976_08185) flgD 1551380..1551811 (+) 432 WP_013352262.1 flagellar hook assembly protein FlgD -
  PF976_RS08190 (PF976_08190) flgG 1551836..1552612 (+) 777 WP_013352263.1 flagellar basal body rod protein FlgG -
  PF976_RS08195 (PF976_08195) - 1552652..1552867 (+) 216 WP_013352264.1 flagellar FlbD family protein -
  PF976_RS08200 (PF976_08200) fliL 1552864..1553283 (+) 420 WP_013352265.1 flagellar basal body-associated protein FliL -
  PF976_RS08205 (PF976_08205) fliM 1553317..1554315 (+) 999 WP_013352266.1 flagellar motor switch protein FliM -
  PF976_RS08210 (PF976_08210) fliY 1554305..1555438 (+) 1134 WP_013352267.1 flagellar motor switch phosphatase FliY -
  PF976_RS08215 (PF976_08215) pilH 1555465..1555827 (+) 363 WP_003154238.1 chemotaxis protein CheY Machinery gene
  PF976_RS08220 (PF976_08220) fliZ 1555842..1556495 (+) 654 WP_013352268.1 flagella biosynthesis regulatory protein FliZ -
  PF976_RS08225 (PF976_08225) fliP 1556488..1557153 (+) 666 WP_013352269.1 flagellar type III secretion system pore protein FliP -
  PF976_RS08230 (PF976_08230) fliQ 1557168..1557437 (+) 270 WP_013352270.1 flagellar biosynthesis protein FliQ -
  PF976_RS08235 (PF976_08235) fliR 1557444..1558223 (+) 780 WP_013352271.1 flagellar biosynthetic protein FliR -
  PF976_RS08240 (PF976_08240) flhB 1558223..1559302 (+) 1080 WP_013352272.1 flagellar biosynthesis protein FlhB -

Sequence

Protein


Download         Length: 120 a.a.        Molecular weight: 13209.53 Da        Isoelectric Point: 4.6805

>NTDB_id=706744 PF976_RS08215 WP_003154238.1 1555465..1555827(+) (pilH) [Bacillus amyloliquefaciens strain SRCM124317]
MAHRILIVDDAAFMRMMIKDILVKNGFDVVAEASDGAQAVEKFKEHSPDLVTMDITMPEMDGITALKEIKQIDPQAKIIM
CSAMGQQSMVIDAIQAGAKDFIVKPFQADRVLEAINKTLS

Nucleotide


Download         Length: 363 bp        

>NTDB_id=706744 PF976_RS08215 WP_003154238.1 1555465..1555827(+) (pilH) [Bacillus amyloliquefaciens strain SRCM124317]
ATGGCACACAGAATTTTAATTGTAGACGATGCAGCATTTATGAGAATGATGATCAAGGATATTTTAGTGAAGAACGGTTT
TGACGTTGTAGCTGAAGCTTCGGACGGAGCACAGGCAGTCGAAAAATTCAAAGAGCATTCACCCGACCTTGTGACAATGG
ATATTACAATGCCTGAAATGGACGGGATTACCGCATTAAAGGAAATTAAACAAATCGATCCGCAGGCGAAAATCATCATG
TGTTCGGCTATGGGGCAGCAGTCAATGGTTATTGACGCGATTCAAGCCGGCGCAAAAGACTTTATCGTGAAGCCATTCCA
AGCAGACCGCGTGCTTGAAGCCATTAATAAAACATTAAGCTAA

Domains

Predicted by InterProScan.

Response regulator receiver domain (Response_reg)

(5-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A150FA43

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilH Synechocystis sp. PCC 6803

38.983

98.333

0.383