Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   NLV76_RS13310 Genome accession   NZ_CP100752
Coordinates   2582923..2583348 (-) Length   141 a.a.
NCBI ID   WP_044154477.1    Uniprot ID   -
Organism   Bacillus halotolerans strain MEC_B301     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2577923..2588348
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NLV76_RS21025 - 2578173..2578307 (-) 135 WP_010335019.1 hypothetical protein -
  NLV76_RS13285 (NLV76_13285) - 2578385..2579116 (-) 732 WP_044154481.1 SGNH/GDSL hydrolase family protein -
  NLV76_RS13290 (NLV76_13290) - 2579363..2580115 (-) 753 WP_059335450.1 N-acetylmuramoyl-L-alanine amidase -
  NLV76_RS13295 (NLV76_13295) - 2580310..2580936 (+) 627 WP_024122122.1 TVP38/TMEM64 family protein -
  NLV76_RS13300 (NLV76_13300) gnd 2580957..2581850 (-) 894 WP_254501000.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  NLV76_RS13305 (NLV76_13305) - 2582171..2582856 (+) 686 Protein_2582 DUF308 domain-containing protein -
  NLV76_RS13310 (NLV76_13310) nucA/comI 2582923..2583348 (-) 426 WP_044154477.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  NLV76_RS13315 (NLV76_13315) sigK 2583525..2584253 (+) 729 WP_254501001.1 RNA polymerase sporulation sigma factor SigK -
  NLV76_RS13320 (NLV76_13320) - 2584390..2584619 (-) 230 Protein_2585 recombinase family protein -
  NLV76_RS13325 (NLV76_13325) - 2584577..2584755 (-) 179 Protein_2586 hypothetical protein -
  NLV76_RS13330 (NLV76_13330) - 2584958..2586193 (-) 1236 WP_254501002.1 cytochrome P450 -
  NLV76_RS13335 (NLV76_13335) - 2586237..2586560 (-) 324 WP_254503641.1 GNAT family N-acetyltransferase -
  NLV76_RS13340 (NLV76_13340) - 2587018..2587578 (+) 561 WP_254501003.1 TetR/AcrR family transcriptional regulator -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15556.74 Da        Isoelectric Point: 5.2215

>NTDB_id=706697 NLV76_RS13310 WP_044154477.1 2582923..2583348(-) (nucA/comI) [Bacillus halotolerans strain MEC_B301]
MKRWMAMLLLAAAVFLCLLGKDMTAPQQVKGAALYDKILYFPLSRYPETGDHIRDAIAEGHSDICTIDRDGADKRREESL
KGIPTKPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTKVLFIVQ

Nucleotide


Download         Length: 426 bp        

>NTDB_id=706697 NLV76_RS13310 WP_044154477.1 2582923..2583348(-) (nucA/comI) [Bacillus halotolerans strain MEC_B301]
ATGAAAAGATGGATGGCAATGCTTCTTCTGGCGGCAGCGGTTTTTCTTTGTTTGTTGGGAAAGGATATGACGGCTCCGCA
ACAGGTCAAGGGGGCTGCTTTATATGACAAAATTTTATACTTTCCGTTGTCTCGTTATCCGGAAACCGGGGATCATATAA
GAGATGCGATTGCCGAAGGACATTCTGATATATGTACAATTGATCGTGATGGAGCGGACAAAAGGCGGGAGGAGTCATTA
AAAGGAATTCCGACGAAGCCTGGCTACGATCGGGATGAATGGCCGATGGCAGTTTGTGAAGAAGGAGGCGCCGGAGCCGA
TGTCCGATATGTAACGCCTTCTGATAATCGCGGCGCCGGTTCATGGGTAGGAAATCAAATGAGCAGTTATCCGGATGGCA
CCAAAGTGTTGTTTATTGTTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(45-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

61.29

87.943

0.539