Detailed information NTG0706690

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: PF976_RS01295
• Genome accession: NZ_CP116011
• Coordinates: 230604..231725 (+)
• Length: 373 a.a.
• NCBI ID: WP_013350919.1
• Uniprot ID: A0A9P1NGF1
• Organism: Bacillus amyloliquefaciens strain SRCM124317
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 225604..236725

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PF976_RS01275 (PF976_01275)	-	225924..226919 (-)	996	WP_013350915.1	DUF5105 domain-containing protein	-
PF976_RS01280 (PF976_01280)	-	227037..228434 (+)	1398	WP_014469698.1	YcdB/YcdC domain-containing protein	-
PF976_RS01285 (PF976_01285)	-	228636..229994 (+)	1359	WP_014469699.1	YcdB/YcdC domain-containing protein	-
PF976_RS01290 (PF976_01290)	-	229991..230494 (-)	504	WP_014469700.1	M15 family metallopeptidase	-
PF976_RS01295 (PF976_01295)	rapF	230604..231725 (+)	1122	WP_013350919.1	tetratricopeptide repeat protein	Regulator
PF976_RS01300 (PF976_01300)	-	231839..232618 (+)	780	WP_013350920.1	glucose 1-dehydrogenase	-
PF976_RS01305 (PF976_01305)	-	232702..234381 (+)	1680	WP_013350921.1	glycoside hydrolase family 13 protein	-
PF976_RS01310 (PF976_01310)	-	234366..234860 (-)	495	WP_013350922.1	DUF4188 domain-containing protein	-
PF976_RS01315 (PF976_01315)	-	234861..235406 (-)	546	WP_013350923.1	PadR family transcriptional regulator	-
PF976_RS01320 (PF976_01320)	-	235615..236574 (+)	960	WP_013350924.1	metal ABC transporter substrate-binding protein	-

Sequence

Protein

• Length: 373 a.a.
• Molecular weight: 44347.46 Da
• Isoelectric Point: 5.0035

>NTDB_id=706690 PF976_RS01295 WP_013350919.1 230604..231725(+) (rapF) [Bacillus amyloliquefaciens strain SRCM124317]
MKSKIPSEEVAVKMNEWYKLIRAFEADKAEALKREIQYELEDMEENQDLLLYFSLMEFRHRIMLDKLKPMKDGDCQPPFS
EMLTEIEMNQQKLTGLLEYYFYYFRGMYEFKQKNFISAISHYKRAEEKLEYVEDDIEKAEFLYKISEVYYHIKQTFFSMN
YAGQALDIYKKYELYGMRRVQCKFIIAGNLMDAQQHEKALEYLECALEHAERLDDHYMIAAAYYNIGHCMYSLGDFCKAA
EDFERAAAGFEAHSFQQVIQAVFSLTHIYFKTERYSDAAASYARGMKSAAEWEDEIYMTKFRLIKELYQGDGGQDSLKEY
FDILESKELLADTEELLQDAAHFFHKRENYKAASYFFSRLVGVQKRLQMNNYA

Nucleotide

• Length: 1122 bp

>NTDB_id=706690 PF976_RS01295 WP_013350919.1 230604..231725(+) (rapF) [Bacillus amyloliquefaciens strain SRCM124317]
ATGAAATCCAAGATTCCATCTGAAGAAGTAGCCGTAAAGATGAATGAATGGTATAAGCTGATCCGCGCGTTTGAAGCCGA
TAAAGCTGAAGCGCTGAAGCGGGAGATTCAATATGAACTCGAAGATATGGAAGAAAATCAGGATTTATTATTGTATTTTT
CATTGATGGAATTTAGGCATCGCATCATGCTGGATAAATTAAAACCCATGAAAGACGGCGACTGCCAGCCTCCGTTCTCT
GAAATGCTCACTGAGATTGAGATGAACCAGCAAAAGCTGACCGGCTTACTGGAATATTATTTTTACTATTTCAGGGGAAT
GTATGAATTTAAGCAGAAAAACTTCATCTCAGCGATTTCTCATTATAAACGGGCTGAGGAAAAACTAGAATATGTGGAAG
ATGACATAGAGAAAGCCGAGTTTCTGTATAAAATTTCTGAAGTGTACTATCATATCAAGCAGACTTTCTTTTCGATGAAT
TACGCGGGACAGGCGCTTGATATTTACAAGAAATATGAATTATACGGAATGAGAAGGGTGCAGTGCAAATTTATCATTGC
CGGTAACCTGATGGACGCCCAGCAGCATGAAAAAGCGCTGGAATACCTTGAATGCGCCTTGGAGCACGCGGAGCGGCTTG
ATGATCACTATATGATTGCTGCGGCCTATTATAATATCGGGCACTGTATGTACAGTTTGGGTGACTTTTGCAAGGCGGCG
GAAGACTTTGAACGAGCGGCTGCCGGTTTTGAAGCGCACAGCTTCCAGCAGGTCATCCAGGCTGTTTTTTCACTCACGCA
TATCTACTTTAAAACGGAAAGATACAGTGACGCCGCCGCCAGCTATGCGCGGGGCATGAAAAGCGCGGCGGAGTGGGAAG
ACGAGATCTATATGACAAAATTCCGTCTGATTAAGGAGCTTTATCAGGGTGACGGCGGACAGGATTCGCTCAAAGAGTAT
TTTGACATTCTGGAATCAAAGGAGCTGTTGGCCGACACTGAGGAACTGCTTCAGGACGCGGCTCATTTCTTTCATAAACG
GGAGAACTACAAAGCGGCATCTTATTTTTTCAGCAGGCTTGTCGGCGTGCAAAAAAGGCTGCAAATGAACAATTACGCAT
AA

Domains

Predicted by InterProScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

• Tetratricopeptide repeat (TPR_12) (187-251)

3D structure

• AlphaFold DB: A0A9P1NGF1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	42.588	99.464	0.424
rapC	Bacillus subtilis subsp. subtilis str. 168	40.323	99.732	0.402