Detailed information    

insolico Bioinformatically predicted

Overview

Name   recF   Type   Machinery gene
Locus tag   NLV76_RS00020 Genome accession   NZ_CP100752
Coordinates   3032..4144 (+) Length   370 a.a.
NCBI ID   WP_254502032.1    Uniprot ID   -
Organism   Bacillus halotolerans strain MEC_B301     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1533..28125 3032..4144 within 0


Gene organization within MGE regions


Location: 1533..28125
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NLV76_RS00010 (NLV76_00010) dnaN 1533..2669 (+) 1137 WP_254502030.1 DNA polymerase III subunit beta -
  NLV76_RS00015 (NLV76_00015) rlbA 2801..3016 (+) 216 WP_003219264.1 ribosome maturation protein RlbA -
  NLV76_RS00020 (NLV76_00020) recF 3032..4144 (+) 1113 WP_254502032.1 DNA replication/repair protein RecF Machinery gene
  NLV76_RS00025 (NLV76_00025) remB 4162..4407 (+) 246 WP_024123583.1 extracellular matrix regulator RemB -
  NLV76_RS00030 (NLV76_00030) gyrB 4463..6379 (+) 1917 WP_254503675.1 DNA topoisomerase (ATP-hydrolyzing) subunit B -
  NLV76_RS00035 (NLV76_00035) gyrA 6591..9062 (+) 2472 WP_254502034.1 DNA topoisomerase (ATP-hydrolyzing) subunit A -
  NLV76_RS00065 (NLV76_00065) - 14455..15405 (-) 951 WP_254503576.1 YaaC family protein -
  NLV76_RS00070 (NLV76_00070) guaB 15526..16992 (+) 1467 WP_254502035.1 IMP dehydrogenase -
  NLV76_RS00075 (NLV76_00075) dacA 17147..18478 (+) 1332 WP_024123588.1 D-alanyl-D-alanine carboxypeptidase -
  NLV76_RS00080 (NLV76_00080) pdxS 18674..19558 (+) 885 WP_254502037.1 pyridoxal 5'-phosphate synthase lyase subunit PdxS -
  NLV76_RS00085 (NLV76_00085) pdxT 19579..20169 (+) 591 WP_254502039.1 pyridoxal 5'-phosphate synthase glutaminase subunit PdxT -
  NLV76_RS00090 (NLV76_00090) serS 20500..21777 (+) 1278 WP_254502041.1 serine--tRNA ligase -
  NLV76_RS00100 (NLV76_00100) dck 22117..22770 (-) 654 WP_254502043.1 deoxyadenosine/deoxycytidine kinase -
  NLV76_RS00105 (NLV76_00105) dgk 22767..23390 (-) 624 WP_254502045.1 deoxyguanosine kinase -
  NLV76_RS00110 (NLV76_00110) - 23489..24772 (-) 1284 WP_254502047.1 glycoside hydrolase family 18 protein -
  NLV76_RS00115 (NLV76_00115) - 24835..25386 (-) 552 WP_254502049.1 isochorismatase family cysteine hydrolase -
  NLV76_RS00120 (NLV76_00120) tadA 25470..25955 (+) 486 WP_024123596.1 tRNA adenosine(34) deaminase TadA -
  NLV76_RS00130 (NLV76_00130) dnaX 26431..28125 (+) 1695 WP_254502051.1 DNA polymerase III subunit gamma/tau -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 42448.47 Da        Isoelectric Point: 7.3310

>NTDB_id=706644 NLV76_RS00020 WP_254502032.1 3032..4144(+) (recF) [Bacillus halotolerans strain MEC_B301]
MYIQNLELTSYRNYEHAELQFENKVNVIIGENAQGKTNLMEAIYVLSMAKSHRTSNDKELIRWDKDYAKIEGRVMKQNGA
IPMQLVISKKGKKGKVNHIEQQKLSQYVGALNTIMFAPEDLNLVKGSPQVRRRFLDMEIGQVSPVYLYDLSLYQKILSQR
NHFLKQLQTRKQTDRTMLDVLTDQLIEVAAKVVVKRLQFTARLEKWAQPIHSGISRGLEELTLKYHTALEVSDPEDLSKI
GDSYQEAFSKLREKEIERGVTLSGPHRDDVLFYVNGRDVQTYGSQGQQRTTALSLKLAEIDLIHEEIGEYPILLLDDVLS
DLDDYRQSHLLHTIQGRVQTFVTTTSVDGIDHETLRQAGMFRVQNGTLVK

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=706644 NLV76_RS00020 WP_254502032.1 3032..4144(+) (recF) [Bacillus halotolerans strain MEC_B301]
TTGTATATCCAGAACTTAGAACTGACATCTTACCGCAACTATGAACATGCTGAGCTTCAATTTGAAAATAAAGTAAATGT
GATCATTGGAGAAAATGCTCAGGGGAAAACAAACCTCATGGAAGCGATCTATGTCTTGTCCATGGCAAAATCGCATCGGA
CTTCAAATGACAAAGAACTTATCCGGTGGGACAAAGACTATGCTAAAATAGAGGGAAGAGTGATGAAGCAAAACGGAGCG
ATCCCGATGCAGCTCGTCATCTCCAAAAAGGGTAAAAAGGGCAAGGTCAATCATATCGAACAGCAAAAGCTCAGCCAATA
TGTCGGCGCCCTCAACACCATCATGTTTGCGCCGGAAGATTTAAACCTTGTAAAGGGAAGCCCTCAAGTGAGACGGAGAT
TTCTTGACATGGAAATCGGCCAAGTTTCTCCTGTCTACCTGTATGACCTTTCTCTTTACCAGAAGATTCTTTCCCAGCGA
AATCATTTTTTGAAACAGCTGCAAACAAGAAAACAAACTGACAGAACAATGCTTGATGTTCTGACTGATCAGCTTATTGA
AGTTGCCGCAAAAGTCGTCGTAAAACGTCTGCAGTTCACGGCACGGCTCGAAAAATGGGCGCAGCCTATCCATTCGGGCA
TCTCAAGAGGGCTTGAGGAATTGACGCTGAAGTACCATACGGCGCTTGAGGTATCAGATCCTGAAGACTTGTCGAAAATA
GGAGATAGCTATCAAGAAGCGTTTTCTAAATTAAGAGAAAAAGAAATCGAGCGCGGCGTCACGCTGTCTGGACCTCATCG
GGATGATGTTCTTTTCTATGTGAACGGACGCGATGTGCAGACGTATGGGTCTCAAGGACAGCAACGGACGACGGCATTAT
CCCTTAAGCTGGCTGAGATTGACCTGATCCATGAAGAAATCGGAGAATATCCCATTTTACTATTGGATGATGTGCTGAGT
GACCTGGATGATTATCGCCAATCGCACTTGCTCCATACCATCCAGGGCCGTGTACAAACGTTTGTCACAACGACAAGCGT
TGATGGCATTGATCACGAAACCTTACGGCAAGCAGGAATGTTCCGTGTGCAAAACGGTACGTTAGTGAAGTGA

Domains

Predicted by InterproScan.

RecF/RecN/SMC N terminal domain (SMC_N)

(2-365)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recF Bacillus subtilis subsp. subtilis str. 168

97.568

100

0.976