Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NL738_RS03850 Genome accession   NZ_CP100722
Coordinates   787284..788264 (+) Length   326 a.a.
NCBI ID   WP_001650641.1    Uniprot ID   A0A418ZC67
Organism   Salmonella enterica subsp. enterica serovar Mbandaka strain R17.4855     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 782284..793264
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NL738_RS03820 (NL738_03820) - 782862..783869 (+) 1008 WP_000252200.1 DUF1202 domain-containing protein -
  NL738_RS03825 (NL738_03825) hemW 783960..785096 (-) 1137 WP_017441078.1 radical SAM family heme chaperone HemW -
  NL738_RS03830 (NL738_03830) - 785089..785682 (-) 594 WP_017441079.1 XTP/dITP diphosphatase -
  NL738_RS03835 (NL738_03835) yggU 785690..785980 (-) 291 WP_001277203.1 DUF167 family protein YggU -
  NL738_RS03840 (NL738_03840) - 785977..786543 (-) 567 WP_001094848.1 YggT family protein -
  NL738_RS03845 (NL738_03845) - 786562..787266 (-) 705 WP_000997812.1 YggS family pyridoxal phosphate-dependent enzyme -
  NL738_RS03850 (NL738_03850) pilT 787284..788264 (+) 981 WP_001650641.1 type IV pilus twitching motility protein PilT Machinery gene
  NL738_RS03855 (NL738_03855) - 788396..789082 (+) 687 WP_017441080.1 IclR family transcriptional regulator domain-containing protein -
  NL738_RS03860 (NL738_03860) ruvX 789128..789544 (-) 417 WP_001285491.1 Holliday junction resolvase RuvX -
  NL738_RS03865 (NL738_03865) - 789544..790107 (-) 564 WP_001053167.1 YqgE/AlgH family protein -
  NL738_RS03870 (NL738_03870) gshB 790323..791270 (-) 948 WP_000593248.1 glutathione synthase -
  NL738_RS03875 (NL738_03875) rsmE 791290..792021 (-) 732 WP_001222488.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NL738_RS03880 (NL738_03880) endA 792098..792805 (-) 708 WP_000286124.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36138.37 Da        Isoelectric Point: 7.5356

>NTDB_id=706466 NL738_RS03850 WP_001650641.1 787284..788264(+) (pilT) [Salmonella enterica subsp. enterica serovar Mbandaka strain R17.4855]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMEIAPFTAPDVDRLLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLALRLLPERCPELAEIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGHLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=706466 NL738_RS03850 WP_001650641.1 787284..788264(+) (pilT) [Salmonella enterica subsp. enterica serovar Mbandaka strain R17.4855]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGATCGCGCCTTTTACCGCGCCAGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ACGCGCAACAATATCAGTGGCGAACGCACGGTCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGTTGTCCTGAACTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAAT
CCACCACGCTGGCGGCGATGGTGGGACATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTGCGTGAAGATCCCGATGTCATTTTGCTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGTCAACTGGCGGGTAGTCTGCGCGCTGTACTATCGCAAAAACTGGAGGT
CGACAGACAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGACTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A418ZC67

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.624

100

0.488
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.624

100

0.488
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter baumannii strain A118

44.954

100

0.451
  pilT Acinetobacter baumannii D1279779

44.954

100

0.451
  pilT Acinetobacter baylyi ADP1

44.954

100

0.451
  pilT Pseudomonas aeruginosa PAK

44.954

100

0.451
  pilT Pseudomonas stutzeri DSM 10701

44.648

100

0.448
  pilT Acinetobacter nosocomialis M2

44.648

100

0.448
  pilT Legionella pneumophila strain Lp02

44.654

97.546

0.436
  pilT Legionella pneumophila strain ERS1305867

44.654

97.546

0.436
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

38.558

97.853

0.377
  pilU Pseudomonas stutzeri DSM 10701

36.336

100

0.371