Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   OYT1_RS02785 Genome accession   NZ_AP018738
Coordinates   584762..586168 (-) Length   468 a.a.
NCBI ID   WP_062625443.1    Uniprot ID   A0A2Z6G9M5
Organism   Ferriphaselus amnicola strain OYT1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 579762..591168
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OYT1_RS02750 (OYT1_ch0569) - 580528..581127 (-) 600 WP_062625449.1 flavin prenyltransferase UbiX -
  OYT1_RS02755 (OYT1_ch0570) - 581124..581654 (-) 531 WP_062625448.1 Gx transporter family protein -
  OYT1_RS02760 (OYT1_ch0571) - 581748..582116 (-) 369 WP_197714104.1 NusG domain II-containing protein -
  OYT1_RS02765 (OYT1_ch0572) rapZ 582121..582960 (-) 840 WP_062625447.1 RNase adapter RapZ -
  OYT1_RS02770 (OYT1_ch0573) hprK 582957..583907 (-) 951 WP_062625446.1 HPr(Ser) kinase/phosphatase -
  OYT1_RS02775 (OYT1_ch0574) - 583922..584377 (-) 456 WP_062625445.1 PTS sugar transporter subunit IIA -
  OYT1_RS02780 (OYT1_ch0575) hpf 584425..584748 (-) 324 WP_062625444.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  OYT1_RS02785 (OYT1_ch0576) rpoN/rpoN1 584762..586168 (-) 1407 WP_062625443.1 RNA polymerase factor sigma-54 Machinery gene
  OYT1_RS02790 (OYT1_ch0577) lptB 586197..586919 (-) 723 WP_062625442.1 LPS export ABC transporter ATP-binding protein -
  OYT1_RS02795 (OYT1_ch0578) lptA 586916..587485 (-) 570 WP_084611879.1 lipopolysaccharide transport periplasmic protein LptA -
  OYT1_RS02800 (OYT1_ch0579) lptC 587469..588092 (-) 624 WP_084611878.1 LPS export ABC transporter periplasmic protein LptC -
  OYT1_RS02805 (OYT1_ch0580) - 588035..588553 (-) 519 WP_062625440.1 HAD family hydrolase -
  OYT1_RS02810 (OYT1_ch0581) - 588553..589539 (-) 987 WP_062625439.1 SIS domain-containing protein -

Sequence

Protein


Download         Length: 468 a.a.        Molecular weight: 53088.85 Da        Isoelectric Point: 4.8422

>NTDB_id=70620 OYT1_RS02785 WP_062625443.1 584762..586168(-) (rpoN/rpoN1) [Ferriphaselus amnicola strain OYT1]
MKASLQLRQSQQLTLTPQLQQSIRLLQLSTLEINQETARLLDENPFLEREEDEITSFATSTASTTSTSPEQPERESSKAE
EDWSELSYSPSSASSDDEDDHYPETAASSPSLREHLLTQLSLSQLDHHDRQLIALLIDALDDGGFLAQSLEDIFEMLPEE
LDVTLDDLETALVQLQHLDEPGIAARDLSECLSLQLRALPATTPSRQLAIQLTDKHLDLLAAHDYTRLKRLLHCDEDGLR
AAQALILQLNPRPAARYEQRVSDYVVPDVIVERQRGRWAARLNPEAMPRLRVNQLYANIMQRRDEQSSQQMTGQLQEARW
YIKNLQQRFDTILRVSQAIVERQRMFLEHGEVAMRPLVLREIAEQLELHESTISRVTTQKFMLTPRGIFELKYFFGSGVS
TDSGGACSSTAIRALIKQLVSQEDAHHPLSDSRLAEILVSQGIQVARRTVAKYRESLNILPVNLRKSL

Nucleotide


Download         Length: 1407 bp        

>NTDB_id=70620 OYT1_RS02785 WP_062625443.1 584762..586168(-) (rpoN/rpoN1) [Ferriphaselus amnicola strain OYT1]
ATGAAAGCATCACTACAACTCAGGCAATCCCAGCAACTGACGCTCACACCGCAGTTGCAGCAATCTATCCGCTTACTGCA
ACTCTCTACCTTGGAGATCAATCAGGAAACTGCGCGACTGCTGGATGAGAATCCGTTCTTGGAGCGCGAGGAAGACGAGA
TCACCTCGTTTGCCACGAGTACGGCAAGCACCACCTCCACCAGCCCCGAGCAGCCTGAAAGAGAATCATCCAAGGCCGAG
GAAGACTGGAGCGAGCTGTCTTATAGCCCTAGCTCAGCCAGCTCTGATGACGAAGATGACCACTATCCCGAGACAGCCGC
CAGCTCCCCAAGTCTGCGCGAGCATCTCCTGACCCAACTCAGCCTCAGCCAACTCGACCACCATGACCGCCAACTGATTG
CACTGCTAATTGACGCGCTAGATGATGGCGGCTTTCTGGCACAGTCATTGGAAGACATCTTCGAGATGCTACCGGAAGAG
TTAGATGTCACGCTAGATGATCTAGAAACTGCACTCGTCCAGTTGCAACATCTAGATGAGCCTGGCATCGCGGCACGCGA
TTTATCTGAGTGTTTGTCCTTGCAGCTGCGCGCCCTGCCAGCCACCACCCCGAGTCGGCAACTAGCGATACAACTCACGG
ACAAGCATCTCGATCTCCTGGCTGCTCACGACTACACCCGCCTCAAACGGCTGCTACACTGTGACGAAGATGGTCTGCGT
GCGGCTCAAGCCCTCATTCTGCAACTCAATCCGCGCCCAGCCGCGCGTTACGAGCAACGGGTCAGCGATTACGTCGTGCC
CGACGTCATCGTCGAGCGCCAGCGTGGTCGCTGGGCGGCCCGCCTCAACCCAGAAGCTATGCCACGTTTACGCGTGAACC
AACTTTACGCCAACATCATGCAGCGCCGTGATGAGCAAAGTTCGCAACAGATGACTGGGCAGTTACAGGAAGCACGTTGG
TACATCAAGAATCTGCAACAGCGCTTCGACACCATCTTGCGCGTATCGCAAGCCATCGTGGAGCGGCAGCGTATGTTCCT
TGAGCATGGCGAAGTCGCCATGCGCCCGCTCGTGCTGCGCGAGATCGCTGAACAGCTTGAATTACACGAATCGACCATTT
CACGAGTCACCACACAGAAGTTCATGCTAACTCCACGTGGTATCTTTGAACTCAAATATTTTTTCGGCAGCGGCGTCTCC
ACCGACAGCGGCGGGGCATGTTCCTCCACCGCCATCCGCGCCTTAATCAAGCAACTGGTCTCTCAGGAAGATGCTCATCA
TCCGCTGAGCGACAGCCGTTTGGCAGAAATCCTCGTGTCGCAGGGCATACAAGTCGCTCGGCGCACTGTGGCAAAATACC
GGGAATCGCTAAATATCCTCCCGGTCAATCTTCGTAAATCACTCTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(311-466)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(104-296)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2Z6G9M5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

53.293

100

0.571

Multiple sequence alignment