Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   NL778_RS03825 Genome accession   NZ_CP100652
Coordinates   844640..846007 (+) Length   455 a.a.
NCBI ID   WP_042111159.1    Uniprot ID   A0A379KHQ5
Organism   Pseudomonas putida strain HSM-C2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 839640..851007
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NL778_RS03795 (NL778_03795) cydB 839847..840854 (+) 1008 WP_012312717.1 cytochrome d ubiquinol oxidase subunit II -
  NL778_RS03800 (NL778_03800) - 840863..841018 (+) 156 WP_080516458.1 DUF2474 domain-containing protein -
  NL778_RS03805 (NL778_03805) - 841051..842175 (-) 1125 WP_254467143.1 methyltransferase -
  NL778_RS03810 (NL778_03810) - 842268..842978 (-) 711 Protein_752 autoinducer binding domain-containing protein -
  NL778_RS03815 (NL778_03815) - 843190..843966 (+) 777 WP_012312721.1 ferredoxin--NADP reductase -
  NL778_RS03820 (NL778_03820) mscL 844043..844462 (-) 420 WP_012312722.1 large-conductance mechanosensitive channel protein MscL -
  NL778_RS03825 (NL778_03825) radA 844640..846007 (+) 1368 WP_042111159.1 DNA repair protein RadA Machinery gene
  NL778_RS03830 (NL778_03830) - 846104..847621 (-) 1518 WP_012312724.1 nucleobase:cation symporter-2 family protein -
  NL778_RS03835 (NL778_03835) - 847885..848250 (-) 366 WP_012312725.1 PilZ domain-containing protein -
  NL778_RS03840 (NL778_03840) - 848363..848998 (-) 636 WP_012312726.1 lytic polysaccharide monooxygenase auxiliary activity family 9 protein -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 48639.04 Da        Isoelectric Point: 6.8985

>NTDB_id=706053 NL778_RS03825 WP_042111159.1 844640..846007(+) (radA) [Pseudomonas putida strain HSM-C2]
MAKAKRLYGCTECGATFPKWAGQCGECGAWNTLVETMIESGGAAAPSGRAGWTGQQAQIKTLAEVSVEEIPRFTTSSTEL
DRVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCNIAVGMPALYVTGEESQQQVAMRSRRLGLPQDQLKVMTETCIETIIA
TARQEKPRVMVIDSIQTIFTEQLQSAPGGVAQVRESAALLVRYAKQSGTAIFLVGHVTKEGSLAGPRVLEHMVDTVLYFE
GESDGRLRLLRAVKNRFGAVNELGVFGMTDRGLKEVSNPSAIFLNRAQEEVPGSVVMATWEGTRPMLVEVQALVDDSHLA
NPRRVTLGLDQNRLAMLLAVLHRHGGIPTHDQDVFLNVVGGVKVLETASDLALLAAVMSSLRNRPLAHGLLVFGEIGLSG
EVRPVPSGQERLKEAAKHGFKRAIVPKGNAPKESPAGLQVIAVTRLEQALDALFE

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=706053 NL778_RS03825 WP_042111159.1 844640..846007(+) (radA) [Pseudomonas putida strain HSM-C2]
ATGGCCAAGGCCAAGCGCTTGTATGGCTGCACTGAGTGTGGCGCGACCTTTCCCAAATGGGCCGGCCAGTGTGGCGAATG
CGGTGCCTGGAACACCCTGGTCGAAACCATGATCGAGAGCGGCGGCGCAGCGGCACCCAGCGGTCGCGCCGGCTGGACCG
GGCAACAGGCGCAGATCAAGACCCTGGCCGAAGTCAGCGTCGAGGAAATTCCGCGGTTCACCACCAGCAGCACCGAACTC
GATCGCGTGCTGGGCGGCGGCCTGGTCGACGGCTCGGTGGTGTTGATCGGTGGTGATCCGGGCATCGGCAAATCGACCAT
TCTGCTGCAGACCTTGTGCAACATTGCTGTGGGCATGCCGGCGCTGTATGTCACCGGCGAGGAGTCGCAGCAGCAGGTGG
CCATGCGTTCACGCCGCCTGGGGCTGCCCCAGGACCAGCTCAAAGTGATGACCGAGACCTGTATTGAAACGATCATCGCT
ACGGCGCGTCAGGAAAAGCCCCGGGTCATGGTCATCGATTCGATCCAGACGATCTTCACCGAGCAATTGCAATCAGCGCC
TGGCGGTGTTGCTCAGGTGCGCGAAAGTGCGGCATTGCTGGTGCGCTACGCCAAGCAGAGCGGCACGGCGATCTTCCTTG
TCGGCCATGTCACCAAGGAAGGCTCGCTGGCGGGCCCGCGGGTGCTGGAACACATGGTCGACACCGTGTTGTATTTCGAG
GGTGAGTCCGATGGCCGCTTGCGCTTGTTGCGCGCGGTGAAGAACCGCTTTGGTGCGGTCAATGAATTGGGTGTCTTCGG
TATGACCGACCGTGGGCTCAAAGAGGTTTCCAACCCTTCGGCGATCTTCCTCAACCGGGCTCAGGAAGAAGTACCGGGCA
GCGTGGTCATGGCCACGTGGGAAGGTACCCGGCCGATGCTGGTAGAGGTACAAGCGCTGGTCGATGACAGCCACCTGGCC
AACCCGCGACGTGTCACCCTGGGCCTGGACCAGAACCGTTTGGCCATGTTGCTGGCGGTGCTGCACCGCCATGGTGGTAT
TCCCACCCATGACCAGGACGTGTTTCTCAATGTCGTCGGTGGGGTGAAGGTCCTTGAAACCGCGTCGGATCTGGCCTTGT
TGGCTGCGGTGATGTCCAGCCTGCGCAACCGGCCGTTGGCCCATGGGCTGCTGGTATTTGGCGAGATTGGTTTGTCGGGC
GAGGTACGGCCGGTTCCCAGTGGGCAGGAGCGCCTGAAAGAAGCGGCGAAACATGGCTTCAAGCGGGCCATCGTGCCCAA
GGGCAACGCACCGAAGGAATCACCGGCGGGGCTGCAGGTGATCGCAGTAACCCGGCTGGAGCAAGCTCTGGACGCCTTGT
TCGAATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(72-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(354-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A379KHQ5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.465

100

0.486
  radA Streptococcus pneumoniae Rx1

45.934

100

0.459
  radA Streptococcus pneumoniae D39

45.934

100

0.459
  radA Streptococcus pneumoniae R6

45.934

100

0.459
  radA Streptococcus pneumoniae TIGR4

45.934

100

0.459
  radA Streptococcus mitis SK321

45.934

100

0.459
  radA Streptococcus mitis NCTC 12261

45.714

100

0.457