Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   NLW79_RS14050 Genome accession   NZ_CP100651
Coordinates   2707921..2708346 (-) Length   141 a.a.
NCBI ID   WP_095713328.1    Uniprot ID   -
Organism   Bacillus halotolerans strain MEC_B334     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2702921..2713346
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NLW79_RS22120 - 2703160..2703294 (-) 135 WP_010335019.1 hypothetical protein -
  NLW79_RS14025 (NLW79_14025) - 2703372..2704103 (-) 732 WP_101864558.1 SGNH/GDSL hydrolase family protein -
  NLW79_RS14030 (NLW79_14030) - 2704350..2705102 (-) 753 WP_127696751.1 N-acetylmuramoyl-L-alanine amidase -
  NLW79_RS14035 (NLW79_14035) - 2705297..2705923 (+) 627 WP_024122122.1 TVP38/TMEM64 family protein -
  NLW79_RS14040 (NLW79_14040) gnd 2705945..2706838 (-) 894 WP_127696750.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  NLW79_RS14045 (NLW79_14045) - 2707159..2707887 (+) 729 WP_127696749.1 DUF308 domain-containing protein -
  NLW79_RS14050 (NLW79_14050) nucA/comI 2707921..2708346 (-) 426 WP_095713328.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  NLW79_RS14055 (NLW79_14055) sigK 2708523..2709251 (+) 729 WP_174754429.1 RNA polymerase sporulation sigma factor SigK -
  NLW79_RS14060 (NLW79_14060) - 2709450..2709653 (-) 204 Protein_2733 recombinase family protein -
  NLW79_RS14065 (NLW79_14065) - 2709640..2709747 (-) 108 Protein_2734 hypothetical protein -
  NLW79_RS14070 (NLW79_14070) - 2710278..2710835 (-) 558 WP_101861070.1 XRE family transcriptional regulator -
  NLW79_RS14075 (NLW79_14075) - 2710979..2712196 (+) 1218 WP_106020130.1 CynX/NimT family MFS transporter -
  NLW79_RS14080 (NLW79_14080) - 2712339..2712471 (-) 133 Protein_2737 recombinase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15496.62 Da        Isoelectric Point: 5.2217

>NTDB_id=706022 NLW79_RS14050 WP_095713328.1 2707921..2708346(-) (nucA/comI) [Bacillus halotolerans strain MEC_B334]
MKRWMAMLLLAAAVFLGLLGKDMTAPQQVKGAALYDKVLYFPLSRYPETGDHIRDAIAEGHSDICTIDRDGADKRREESL
KGIPTKPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTKVLFIVQ

Nucleotide


Download         Length: 426 bp        

>NTDB_id=706022 NLW79_RS14050 WP_095713328.1 2707921..2708346(-) (nucA/comI) [Bacillus halotolerans strain MEC_B334]
ATGAAAAGATGGATGGCAATGCTTCTTCTGGCGGCAGCAGTTTTTCTTGGTTTATTGGGAAAGGATATGACGGCTCCGCA
ACAGGTCAAGGGGGCTGCTTTATATGACAAAGTTTTATATTTTCCGTTGTCTCGTTATCCGGAAACCGGGGATCATATAA
GAGATGCGATTGCCGAAGGACATTCTGATATATGTACAATTGATCGTGATGGAGCGGACAAAAGGCGGGAGGAATCATTA
AAAGGAATTCCGACGAAGCCTGGCTACGATCGGGATGAATGGCCGATGGCAGTTTGTGAAGAAGGAGGTGCCGGAGCCGA
TGTCCGATATGTAACGCCTTCTGATAATCGCGGCGCCGGTTCATGGGTAGGAAATCAAATGAGCAGTTATCCAGATGGCA
CCAAAGTGTTGTTTATTGTTCAGTGA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(45-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

61.905

89.362

0.553