Detailed information    

insolico Bioinformatically predicted

Overview

Name   recF   Type   Machinery gene
Locus tag   NLW79_RS00020 Genome accession   NZ_CP100651
Coordinates   3032..4144 (+) Length   370 a.a.
NCBI ID   WP_024123582.1    Uniprot ID   -
Organism   Bacillus halotolerans strain MEC_B334     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1533..28050 3032..4144 within 0


Gene organization within MGE regions


Location: 1533..28050
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NLW79_RS00010 (NLW79_00010) dnaN 1533..2669 (+) 1137 WP_024123581.1 DNA polymerase III subunit beta -
  NLW79_RS00015 (NLW79_00015) rlbA 2801..3016 (+) 216 WP_003219264.1 ribosome maturation protein RlbA -
  NLW79_RS00020 (NLW79_00020) recF 3032..4144 (+) 1113 WP_024123582.1 DNA replication/repair protein RecF Machinery gene
  NLW79_RS00025 (NLW79_00025) remB 4162..4407 (+) 246 WP_024123583.1 extracellular matrix regulator RemB -
  NLW79_RS00030 (NLW79_00030) gyrB 4463..6379 (+) 1917 WP_254518329.1 DNA topoisomerase (ATP-hydrolyzing) subunit B -
  NLW79_RS00035 (NLW79_00035) gyrA 6591..9062 (+) 2472 WP_024123585.1 DNA topoisomerase (ATP-hydrolyzing) subunit A -
  NLW79_RS00065 (NLW79_00065) - 14379..15329 (-) 951 WP_151175186.1 YaaC family protein -
  NLW79_RS00070 (NLW79_00070) guaB 15450..16916 (+) 1467 WP_024123587.1 IMP dehydrogenase -
  NLW79_RS00075 (NLW79_00075) dacA 17071..18402 (+) 1332 WP_024123588.1 D-alanyl-D-alanine carboxypeptidase -
  NLW79_RS00080 (NLW79_00080) pdxS 18599..19483 (+) 885 WP_024123589.1 pyridoxal 5'-phosphate synthase lyase subunit PdxS -
  NLW79_RS00085 (NLW79_00085) pdxT 19504..20094 (+) 591 WP_106021713.1 pyridoxal 5'-phosphate synthase glutaminase subunit PdxT -
  NLW79_RS00090 (NLW79_00090) serS 20426..21703 (+) 1278 WP_254517981.1 serine--tRNA ligase -
  NLW79_RS00100 (NLW79_00100) dck 22042..22695 (-) 654 WP_024123592.1 deoxyadenosine/deoxycytidine kinase -
  NLW79_RS00105 (NLW79_00105) dgk 22692..23315 (-) 624 WP_254517982.1 deoxyguanosine kinase -
  NLW79_RS00110 (NLW79_00110) - 23414..24697 (-) 1284 WP_038954334.1 LysM peptidoglycan-binding domain-containing protein -
  NLW79_RS00115 (NLW79_00115) - 24760..25311 (-) 552 WP_010332741.1 isochorismatase family cysteine hydrolase -
  NLW79_RS00120 (NLW79_00120) tadA 25395..25880 (+) 486 WP_024123596.1 tRNA adenosine(34) deaminase TadA -
  NLW79_RS00130 (NLW79_00130) dnaX 26356..28050 (+) 1695 WP_254517983.1 DNA polymerase III subunit gamma/tau -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 42434.44 Da        Isoelectric Point: 7.0882

>NTDB_id=705968 NLW79_RS00020 WP_024123582.1 3032..4144(+) (recF) [Bacillus halotolerans strain MEC_B334]
MYIQNLELTSYRNYEHAELQFENKVNVIIGENAQGKTNLMEAIYVLSMAKSHRTSNDKELIRWDKDYAKIEGRVMKQNGA
IPMQLVISKKGKKGKVNHIEQQKLSQYVGALNTIMFAPEDLNLVKGSPQVRRRFLDMEIGQVSPVYLYDLSLYQKILSQR
NHFLKQLQTRKQTDRTMLDVLTDQLIEVAAKVVVKRLQFTAQLEKWAQPIHSGISRGLEELTLKYHTALEVSDPEDLSKI
GDSYQEAFSKLREKEIERGVTLSGPHRDDVLFYVNGRDVQTYGSQGQQRTTALSLKLAEIDLIHEEIGEYPILLLDDVLS
ELDDYRQSHLLHTIQGRVQTFVTTTSVDGIDHETLRQAGMFRVQNGTLVK

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=705968 NLW79_RS00020 WP_024123582.1 3032..4144(+) (recF) [Bacillus halotolerans strain MEC_B334]
TTGTATATCCAGAACTTAGAACTGACATCTTACCGCAACTATGAACATGCTGAACTTCAATTTGAAAATAAAGTAAATGT
GATCATTGGAGAAAATGCTCAGGGGAAAACAAACCTCATGGAAGCGATCTATGTCTTGTCCATGGCAAAATCGCATCGGA
CTTCAAATGACAAAGAACTTATCCGGTGGGACAAAGACTATGCTAAAATAGAGGGAAGAGTGATGAAGCAAAACGGGGCG
ATCCCGATGCAGCTCGTCATCTCCAAAAAGGGTAAAAAGGGCAAGGTCAATCATATCGAACAGCAAAAGCTCAGCCAGTA
TGTCGGCGCCCTCAACACCATCATGTTTGCACCGGAAGATTTAAACCTTGTAAAGGGAAGCCCTCAAGTGAGACGGAGAT
TTCTTGACATGGAAATCGGCCAAGTTTCTCCCGTCTACCTGTATGACCTTTCTCTTTACCAGAAGATTCTTTCCCAGCGA
AATCATTTTTTGAAACAGCTGCAAACAAGAAAACAAACTGACAGAACAATGCTTGATGTTCTGACTGATCAGCTTATTGA
AGTTGCCGCAAAAGTCGTCGTAAAACGTCTGCAGTTCACGGCACAGCTCGAAAAATGGGCGCAGCCTATCCATTCGGGCA
TCTCAAGAGGGCTTGAGGAATTGACGCTGAAGTACCATACGGCGCTTGAGGTATCAGATCCTGAAGACTTGTCGAAAATA
GGAGATAGCTATCAAGAAGCGTTTTCTAAATTAAGAGAAAAAGAAATCGAGCGCGGCGTCACGCTGTCTGGACCTCATCG
GGATGATGTTCTTTTCTATGTGAACGGACGCGATGTGCAGACGTATGGGTCTCAAGGACAGCAGCGGACGACGGCATTAT
CCCTTAAGCTGGCTGAGATTGACCTGATCCATGAAGAAATCGGAGAATATCCCATTTTACTATTGGATGATGTGCTGAGT
GAACTGGATGATTATCGCCAATCGCACTTGCTTCATACCATCCAGGGCCGTGTACAAACGTTTGTCACAACGACAAGCGT
TGATGGCATTGATCACGAAACCTTACGGCAAGCAGGAATGTTCCGTGTGCAAAACGGTACGTTAGTGAAGTGA

Domains

Predicted by InterproScan.

RecF/RecN/SMC N terminal domain (SMC_N)

(2-365)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recF Bacillus subtilis subsp. subtilis str. 168

98.108

100

0.981